Small secreted effector protein from Fusarium sacchari suppresses host immune response by inhibiting ScPi21‐induced cell death
Molecular Plant Pathology,
Год журнала:
2024,
Номер
25(1)
Опубликована: Янв. 1, 2024
Fusarium
sacchari
is
one
of
the
primary
pathogens
causing
pokkah
boeng
disease,
which
impairs
yield
and
quality
sugarcane
around
world.
Understanding
molecular
mechanisms
F.
effectors
that
regulate
plant
immunity
great
importance
for
development
novel
strategies
persistent
control
disease.
In
a
previous
study,
Fs00367
was
identified
to
inhibit
BAX-induced
cell
death.
this
Fs00367nsp
(without
signal
peptide)
found
suppress
death,
reactive
oxygen
species
bursts
callose
accumulation.
The
amino
acid
region
113-142
functional
region.
Gene
mutagenesis
indicated
important
full
virulence
sacchari.
A
yeast
two-hybrid
assay
revealed
an
interaction
between
ScPi21
in
further
confirmed
using
bimolecular
fluorescence
complementation,
pull-down
co-immunoprecipitation.
can
induce
immunity,
but
effect
could
be
blunted
by
Fs00367nsp.
These
results
suggest
core
pathogenicity
factor
suppresses
through
inhibiting
ScPi21-induced
findings
study
provide
new
insights
into
regulating
immunity.
Язык: Английский
Fusarium sacchari Effector FsMEP1 Contributes to Virulence by Disturbing Localization of Thiamine Thiazole Synthase ScTHI2 from Sugarcane
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(22), С. 12075 - 12075
Опубликована: Ноя. 10, 2024
is
a
significant
pathogenic
fungus
that
causes
sugarcane
Pokkah
Boeng.
Proteins
secreted
by
fungi
can
be
delivered
into
hosts
to
suppress
plant
immunity
and
establish
infection.
However,
there
still
much
discovered
regarding
Язык: Английский
Fusarium sacchari CFEM Proteins Suppress Host Immunity and Differentially Contribute to Virulence
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12805 - 12805
Опубликована: Ноя. 28, 2024
The
pathogen
Fusarium
sacchari
is
responsible
for
the
devastating
pokkah
boeng
disease,
which
causes
significant
economic
losses
in
sugarcane
production.
However,
mechanisms
by
it
affects
plant
immunity
remain
largely
unknown.
Common
Fungal
Extracellular
Membrane
(CFEM)
domain
proteins
have
been
implicated
fungal
growth,
infection
processes,
and
pathogenicity.
In
this
study,
we
identified
three
FsCFEM
(Fs08184,
Fs10706,
Fs13617)
that
mediate
broad-spectrum
suppression
of
immune
responses
induced
typical
effectors.
A
further
analysis
demonstrated
Fs08184,
Fs13617
suppressed
host
through
two
potential
iron-binding
sites
conserved
CFEM
family
members,
characterized
Asp
Phe
residues
Fs13617.
Additionally,
within
iron-chelating
site
were
necessary
iron
acquisition
F.
contributed
to
creating
low-free-iron
conditions
at
interface
interactions.
It
appeared
might
employ
Asp-Phe-type
members
influence
homeostasis
suppress
facilitate
its
successful
colonization.
Язык: Английский