Fusarium sacchari CFEM Proteins Suppress Host Immunity and Differentially Contribute to Virulence DOI Open Access

Tianshu Hong,

Shichao Wang,

Zhiyuan Luo

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12805 - 12805

Опубликована: Ноя. 28, 2024

The pathogen Fusarium sacchari is responsible for the devastating pokkah boeng disease, which causes significant economic losses in sugarcane production. However, mechanisms by it affects plant immunity remain largely unknown. Common Fungal Extracellular Membrane (CFEM) domain proteins have been implicated fungal growth, infection processes, and pathogenicity. In this study, we identified three FsCFEM (Fs08184, Fs10706, Fs13617) that mediate broad-spectrum suppression of immune responses induced typical effectors. A further analysis demonstrated Fs08184, Fs13617 suppressed host through two potential iron-binding sites conserved CFEM family members, characterized Asp Phe residues Fs13617. Additionally, within iron-chelating site were necessary iron acquisition F. contributed to creating low-free-iron conditions at interface interactions. It appeared might employ Asp-Phe-type members influence homeostasis suppress facilitate its successful colonization.

Язык: Английский

Small secreted effector protein from Fusarium sacchari suppresses host immune response by inhibiting ScPi21‐induced cell death DOI Creative Commons
Zhen Huang, Caixia Wang, Huixue Li

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(1)

Опубликована: Янв. 1, 2024

Fusarium sacchari is one of the primary pathogens causing pokkah boeng disease, which impairs yield and quality sugarcane around world. Understanding molecular mechanisms F. effectors that regulate plant immunity great importance for development novel strategies persistent control disease. In a previous study, Fs00367 was identified to inhibit BAX-induced cell death. this Fs00367nsp (without signal peptide) found suppress death, reactive oxygen species bursts callose accumulation. The amino acid region 113-142 functional region. Gene mutagenesis indicated important full virulence sacchari. A yeast two-hybrid assay revealed an interaction between ScPi21 in further confirmed using bimolecular fluorescence complementation, pull-down co-immunoprecipitation. can induce immunity, but effect could be blunted by Fs00367nsp. These results suggest core pathogenicity factor suppresses through inhibiting ScPi21-induced findings study provide new insights into regulating immunity.

Язык: Английский

Процитировано

3

Fusarium sacchari Effector FsMEP1 Contributes to Virulence by Disturbing Localization of Thiamine Thiazole Synthase ScTHI2 from Sugarcane DOI Open Access
Lulu Wang, Deng Wu,

Tianshu Hong

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12075 - 12075

Опубликована: Ноя. 10, 2024

is a significant pathogenic fungus that causes sugarcane Pokkah Boeng. Proteins secreted by fungi can be delivered into hosts to suppress plant immunity and establish infection. However, there still much discovered regarding

Язык: Английский

Процитировано

0

Fusarium sacchari CFEM Proteins Suppress Host Immunity and Differentially Contribute to Virulence DOI Open Access

Tianshu Hong,

Shichao Wang,

Zhiyuan Luo

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12805 - 12805

Опубликована: Ноя. 28, 2024

The pathogen Fusarium sacchari is responsible for the devastating pokkah boeng disease, which causes significant economic losses in sugarcane production. However, mechanisms by it affects plant immunity remain largely unknown. Common Fungal Extracellular Membrane (CFEM) domain proteins have been implicated fungal growth, infection processes, and pathogenicity. In this study, we identified three FsCFEM (Fs08184, Fs10706, Fs13617) that mediate broad-spectrum suppression of immune responses induced typical effectors. A further analysis demonstrated Fs08184, Fs13617 suppressed host through two potential iron-binding sites conserved CFEM family members, characterized Asp Phe residues Fs13617. Additionally, within iron-chelating site were necessary iron acquisition F. contributed to creating low-free-iron conditions at interface interactions. It appeared might employ Asp-Phe-type members influence homeostasis suppress facilitate its successful colonization.

Язык: Английский

Процитировано

0