Plant
volatile
aldehydes
(PVAs)
such
as
cinnamaldehyde
(Cin),
citral
(Cit),
citronellal
(Citr),
and
perillaldehyde
(Per)
have
broad-spectrum
antimicrobial
activity
show
great
potential
in
agricultural
sustainable
production.
However,
most
PVAs
not
only
very
high
volatility
but
also
are
easily
degradable
environment,
which
seriously
restricts
their
wide
application.
To
address
the
inherent
problems
with
PVAs,
four
prodrugs
based
on
fabricated
by
conjugating
individually
Cin,
Cit,
Citr,
Per
to
sodium
bisulfite
(Sod)
through
a
simple
addition
reaction
subsequently
self-assembled
into
nanoparticles
(prodrug
self-assemblies)
aqueous
solutions.
The
results
showed
that
pH
of
7
temperature
35
°C
optimal
conditions
for
formation
prodrug
self-assemblies
highest
self-assembly
rates.
prepared
spherical
average
particle
sizes
100-200
nm,
almost
no
volatilization,
surface
stability,
can
respond
acidic
redox
microenvironments
release
PVAs.
synergistic
activities
against
Sclerotinia
sclerotiorum
Penicillium
digitatum,
good
biological
safety
plants.
Therefore,
these
findings
important
implications
efficient
utilization
agriculture,
ensuring
ecological
environment
realizing
development
agriculture.
Molecular Plant Pathology,
Год журнала:
2024,
Номер
25(2)
Опубликована: Фев. 1, 2024
Sclerotinia
sclerotiorum
is
a
cosmopolitan
and
typical
necrotrophic
phytopathogenic
fungus
that
infects
hundreds
of
plant
species.
Because
no
cultivars
highly
resistant
to
S.
are
available,
managing
disease
caused
by
still
challenging.
However,
recent
studies
have
demonstrated
has
beneficial
effect
can
live
mutualistically
as
an
endophyte
in
graminaceous
plants,
protecting
the
plants
against
major
fungal
diseases.
An
in-depth
understanding
schizotrophic
lifestyle
during
interactions
with
under
different
environmental
conditions
will
provide
new
strategies
for
controlling
disease.
In
this
review,
we
summarize
pathogenesis
mechanisms
its
attack
host
destructive
pathogen
discuss
endophytic
fungus.
ABSTRACT
Hypovirulence-associated
mycoviruses
have
the
potential
as
biocontrol
agents
for
plant
fungal
disease
management,
and
exploration
of
interactions
between
these
phytopathogenic
fungi
can
provide
opportunities
to
elucidate
underlying
mechanisms
hypovirulence
antiviruses.
We
previously
found
that
Sclerotinia
sclerotiorum
endornavirus
3
(SsEV3),
belonging
genus
Betaendornavirus
within
family
Endornaviridae
,
confers
on
fungus
but
remains
unclear.
In
this
study,
we
SsEV3-infected
strain
produced
fewer
sclerotia,
failed
form
infection
cushions
hosts,
exhibited
increased
cell
vacuolation,
was
more
sensitive
abiotic
stresses.
SsEV3
evoked
transcriptional
rewiring
in
S.
affecting
genes
related
virulence
factors
pathogenicity
RNAi
pathway
An
unknown
biological
function
gene
Sssnf1
downregulated
following
infection.
Deletion
impaired
cushion
formation
decreased
.
Five
key
RNAi-related
were
significantly
upregulated,
deletion
Ssdcl2
contributed
accumulation.
Additionally,
identified
a
hypothetical
protein
encoded
by
Sshp1
directly
interacts
with
RNA-dependent
RNA
polymerase
(RdRp)
domain
SsEV3.
Although
mutants
normal
colony
morphology,
they
showed
higher
accumulation
reduced
resistance
reactive
oxygen
species,
indicating
similar
genes,
plays
an
antiviral
role
response
may
represent
new
antivirus
factor.
Therefore,
examination
interaction
provides
insights
into
fungi.
IMPORTANCE
emerged
promising
agents,
studying
their
helps
uncover
pathogenesis
defense.
This
study
critical
its
hypovirulence-associated
endornavirus,
SsEV3,
elucidating
molecular
mycovirus-induced
changes
not
only
impairs
traits,
including
sclerotial
production
also
triggers
host
responses
involving
typical
interference
pathways.
New
factors,
such
based
established
system
endornavirus.
These
findings
deepen
our
understanding
fungus-mycovirus
interactions,
highlighting
reducing
facilitating
development
mycovirus-based
control
strategies.
Molecular Plant Pathology,
Год журнала:
2025,
Номер
26(4)
Опубликована: Март 28, 2025
ABSTRACT
GPI‐anchored
proteins
are
widely
distributed
in
eukaryotic
cells.
However,
their
functions
still
poorly
understood
necrotrophic
pathogenic
fungi.
Here,
based
on
Agrobacterium
tumefaciens
‐mediated
transient
expression
screening,
a
novel
secreted
protein,
SsGP1,
that
induces
plant
cell
death
was
characterised
Sclerotinia
sclerotiorum
.
The
homologues
of
SsGP1
broadly
among
ascomycetes.
can
activate
immune
responses,
including
reactive
oxygen
species
(ROS)
burst
and
the
up‐regulated
immunity
genes,
manner
is
dependent
BAK1
but
independent
SOBIR1.
Treatment
plants
with
protein
enhanced
resistance
Nicotiana
benthamiana
Arabidopsis
thaliana
to
S.
Our
findings
reveal
as
pathogen‐associated
molecular
pattern
(PAMP)
recognised
by
BAK1‐dependent
manner.
Molecular Plant Pathology,
Год журнала:
2025,
Номер
26(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Microorganisms
use
versatile
strategies
to
facilitate
the
colonisation
of
hosts,
through
remodelling
transcription
and
metabolism
accommodate
growth
under
harsh
hostile
environments.
Sclerotinia
sclerotiorum
is
a
typical
necrotrophic
pathogen
that
causes
stem
rot
in
more
than
700
species,
resulting
serious
economic
losses.
How
S.
integrates
mechanisms
for
nutrient
acquisition
utilisation
maintain
optimal
pathogenicity
still
indistinct.
Here,
we
demonstrate
Ndt80
family
factors
(SsNdt80a,b,c)
are
involved
carbon
source
have
different
roles
growth,
sclerotia
formation,
infection
cushion
development,
virulence
.
SsNdt80b
could
bind
promoter
SsSNF1
modulate
transcriptional
activity
SsSNF1.
Silencing
resulted
defects
hyphal
reduced
cell
wall‐degrading
enzymes,
A
model
proposed
which
responds
sources
modulates
SsSnf1
regulate
development
