Turnip mosaic virus NIb weakens the function of eukaryotic translation initiation factor 6 facilitating viral infection in Nicotiana benthamiana DOI Creative Commons
Ziqiang Chen, Feng Wang, Binghua Chen

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(2)

Опубликована: Фев. 1, 2024

Viruses rely completely on host translational machinery to produce the proteins encoded by their genes. Controlling translation initiation is important for gaining advantage in conflicts between and virus. The eukaryotic factor 4E (eIF4E) has been reported be hijacked potyviruses virus multiplication. role of regulation defence anti-defence plants viruses not well understood. We report that transcript level eIF6 was markedly increased turnip mosaic (TuMV)-infected Nicotiana benthamiana. TuMV infection impaired overexpression N. benthamiana (NbeIF6) either transiently expressed leaves or stably transgenic plants. Polysome profile assays showed NbeIF6 caused accumulation 40S 60S ribosomal subunits, reduction polysomes, also compromised UTR-mediated translation, indicating a upregulated during infection. However, polysome TuMV-infected identical overexpressing NbeIF6. Further analysis NIb protein, RNA-dependent RNA polymerase, interacted with interfered its effect suggesting might have counterdefence role. results propose possible regulatory mechanism at plant-virus interaction.

Язык: Английский

Construction of watermelon mosaic virus-Beijing isolate infectious clone and study of the dynamic localization and accumulation of virus-encoded proteins DOI

Mengjiao Pan,

Linhao Ge,

Weifang Gao

и другие.

Virology, Год журнала: 2025, Номер unknown, С. 110571 - 110571

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Turnip mosaic virus NIb weakens the function of eukaryotic translation initiation factor 6 facilitating viral infection in Nicotiana benthamiana DOI Creative Commons
Ziqiang Chen, Feng Wang, Binghua Chen

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(2)

Опубликована: Фев. 1, 2024

Viruses rely completely on host translational machinery to produce the proteins encoded by their genes. Controlling translation initiation is important for gaining advantage in conflicts between and virus. The eukaryotic factor 4E (eIF4E) has been reported be hijacked potyviruses virus multiplication. role of regulation defence anti-defence plants viruses not well understood. We report that transcript level eIF6 was markedly increased turnip mosaic (TuMV)-infected Nicotiana benthamiana. TuMV infection impaired overexpression N. benthamiana (NbeIF6) either transiently expressed leaves or stably transgenic plants. Polysome profile assays showed NbeIF6 caused accumulation 40S 60S ribosomal subunits, reduction polysomes, also compromised UTR-mediated translation, indicating a upregulated during infection. However, polysome TuMV-infected identical overexpressing NbeIF6. Further analysis NIb protein, RNA-dependent RNA polymerase, interacted with interfered its effect suggesting might have counterdefence role. results propose possible regulatory mechanism at plant-virus interaction.

Язык: Английский

Процитировано

2