Membrane fluidity control by the Magnaporthe oryzae acyl-CoA binding protein sets the thermal range for host rice cell colonization DOI Creative Commons
Michael Richter, Lauren Segal, Raquel O. Rocha

и другие.

PLoS Pathogens, Год журнала: 2024, Номер 20(11), С. e1012738 - e1012738

Опубликована: Ноя. 25, 2024

Following leaf cuticle penetration by specialized appressorial cells, the devastating blast fungus Magnaporthe oryzae grows as invasive hyphae (IH) in living rice cells. IH are separated from host cytoplasm plant-derived membranes forming an apoplastic compartment and a punctate biotrophic interfacial complex (BIC) that mediate molecular host-pathogen interaction. What cellular processes determine temperature range for this growth stage is unanswered question pertinent to broader understanding of how phytopathogens may cope with environmental stresses arising under climate change. Here, we shed light on thermal adaptation M. disrupting ACB1 gene encoding single acyl-CoA-binding protein, intracellular transporter long-chain acyl-CoA esters. Loss affected fatty acid desaturation levels abolished pathogenicity at optimal (26°C) low (22°C) but not elevated (29°C) infection temperatures (the latter following post-penetration shifts 26°C). Relative wild type, Δacb1 mutant strain exhibited poor vegetative impaired membrane trafficking 22°C 26°C, 29°C. In planta, was inhibited 26°C-which accompanied multi-BIC phenotype-but 29°C, where BIC formation normal. Underpinning phenotype fluidity 26°C temperatures, indicating Acb1 suppresses rigidity optimal- suboptimal- supraoptimal temperatures. Deducing temperature-dependent role maintaining homeostasis reveals disease both mechanistically determined wider than hitherto appreciated.

Язык: Английский

The unconventional resistance protein PTR recognizes the Magnaporthe oryzae effector AVR-Pita in an allele-specific manner DOI
Gui Xiao,

Nutthalak Laksanavilat,

Stella Césari

и другие.

Nature Plants, Год журнала: 2024, Номер 10(6), С. 994 - 1004

Опубликована: Июнь 4, 2024

Язык: Английский

Процитировано

10
Pyricularia oryzae: Lab star and field scourge DOI Creative Commons
Maël Baudin, Marie Le Naour—Vernet, Pierre Gladieux

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(4)

Опубликована: Апрель 1, 2024

Abstract Pyricularia oryzae (syn. Magnaporthe ), is a filamentous ascomycete that causes major disease called blast on cereal crops, as well wide variety of wild and cultivated grasses. Blast diseases have tremendous impact worldwide particularly rice wheat, where the emerged in South America 1980s, before spreading to Asia Africa. Its economic importance, coupled with its amenability molecular genetic manipulation, inspired extensive research efforts aiming at understanding biology evolution. In past 40 years, this plant‐pathogenic fungus has model plant–microbe interactions. review, we focus clarification taxonomy structure species host range determinants. We also discuss recent studies deciphering lifecycle. Taxonomy Kingdom: Fungi , phylum: Ascomycota sub‐phylum: Pezizomycotina class: Sordariomycetes order: Magnaporthales family: Pyriculariaceae genus: Pyricularia. Host P. ability infect Poaceae . It structured into different host‐specialized lineages are each associated few plant genera. The best known cause damage but it can attack other economically important crops such maize, barley, finger millet. Disease symptoms necrotic lesions or bleaching all aerial parts plants, including leaf blades, sheaths, inflorescences (panicles, spikes, seeds). Characteristic leaves diamond‐shaped silver often brown margin whose appearance influenced by numerous factors genotype environmental conditions. USEFUL WEBSITES Resources URL Genomic data repositories http://genome.jouy.inra.fr/gemo/ http://openriceblast.org/ http://openwheatblast.net/ Genome browser for fungi (including ) http://fungi.ensembl.org/index.html Comparative genomics database https://mycocosm.jgi.doe.gov/mycocosm/home T‐DNA mutant http://atmt.snu.kr/ http://www.phi‐base.org/ SNP expression https://fungidb.org/fungidb/app/

Язык: Английский

Процитировано

8
The Role of Genetic Resistance in Rice Disease Management DOI Open Access
Andrews Danso Ofori, Tengda Zheng, John Kwame Titriku

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 956 - 956

Опубликована: Янв. 23, 2025

Rice (Oryza sativa) is a crucial staple crop for global food security, particularly in Asia. However, rice production faces significant challenges from various diseases that can cause substantial yield losses. This review explores the role of genetic resistance disease management, focusing on molecular mechanisms underlying plant–pathogen interactions and strategies developing resistant varieties. The paper discusses qualitative quantitative resistance, emphasizing importance (R) genes, defense-regulator trait loci (QTLs) conferring broad-spectrum resistance. Gene-for-gene relationships rice–pathogen are examined, Xanthomonas oryzae pv. Magnaporthe oryzae. also covers recent advancements breeding techniques, including marker-assisted selection, engineering, genome editing technologies like CRISPR-Cas. These approaches offer promising avenues enhancing while maintaining potential. Understanding exploiting durable disease-resistant varieties, essential ensuring sustainable security face evolving pathogen threats changing environmental conditions.

Язык: Английский

Процитировано

0
Evolutionary genomics reveals variation in structure and genetic content implicated in virulence and lifestyle in the genus Gaeumannomyces DOI Creative Commons
Rowena Hill, Michelle Grey, Mariano Olivera Fedi

и другие.

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Март 12, 2025

Abstract Gaeumannomyces tritici is responsible for take-all disease, one of the most important wheat root threats worldwide. High-quality annotated genome resources are sorely lacking this pathogen, as well closely related antagonist and potential biocontrol agent, G. hyphopodioides . As such, we know very little about genetic basis interactions in host–pathogen–antagonist system. Using PacBio HiFi sequencing technology have generated nine near-complete assemblies, including two different virulence lineages first assemblies avenae (oat take-all). Genomic signatures support presence distinct (types A B), with strains potentially employing a mechanism to prevent gene copy-number expansions. The CAZyme repertoire was highly conserved across , while candidate secreted effector proteins biosynthetic clusters showed more variability may distinguish pathogenic non-pathogenic lineages. transition from self-sterility (heterothallism) self-fertility (homothallism) also be key innovation implicated lifestyle. We did not find evidence transposable element compartmentalisation genus, however Starship giant elements contribute genomic plasticity genus. Our results depict an ideal system explore within rhizosphere, nuances intraspecific virulence, interspecific antagonism, fungal lifestyle evolution. foundational provided here will enable development diagnostics surveillance understudied but agriculturally pathogens.

Язык: Английский

Процитировано

0
Immune regulatorsEDS1andPAD4constrainADR1-dependent disease resistance in rice DOI Creative Commons
Joel Fernandes, Junli Wang, Rong Su

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 8, 2024

Withdrawal Statement The authors have withdrawn this manuscript owing to finding inconsistencies in genotyping of kitaake rice eds1 pad4 double mutant lines and establishing that combined loss EDS1 PAD4 does not underlie the stunted ‘autoimmune-like’ phenotype reported preprint. Therefore, do wish work be cited as reference for project. If you any questions, please contact corresponding authors: Jane Parker, Thomas Kroj Haitao Cui (co-corresponding)

Язык: Английский

Процитировано

0
Ecological Role of Phytotoxic Secondary Metabolites Produced by Phytopathogenic Fungi DOI
Alexander Berestetskiy, Qiongbo Hu

Reference series in phytochemistry, Год журнала: 2024, Номер unknown, С. 1 - 24

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0
Synthesis of Novel Chromene Derivatives Bearing Hydrazide/Thiazol/Oxazol/Oxime Moieties as Potential Antifungal Agents DOI
Yong Li,

Taigui Ma,

Ya Yang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 20, 2024

Four series of novel hydrazide/thiazol/oxazol/oxime ester hybrids chromene derivatives were designed and synthesized to explore natural-product-based fungicide candidates. Preliminary antifungal activity assay results demonstrated that hydrazide-chromene thiazol-chromene exhibited excellent broad-spectrum inhibitory against ten phytopathogenic fungi. Among them, six compounds 4b, 4c, 4d, 4e, 4h, 4l displayed the most remarkable effects. Notably, 4e showed comparable protective curative effects with chlorothalonil potatoes cherry tomatoes infected by Fusarium solani Botrytis cinerea, respectively. Meanwhile, compound also exerted potential rice pepper leaves Pyricularia oryzae Phytophthora capsici, Additionally, a preliminary mechanism study revealed could significantly inhibit germination spores promote increased mycelium permeability content leakage disrupting fungal membrane structure. The in vitro cytotoxicity indicated almost all possessed relatively low cytotoxicity. These findings provide foundation for application chromene-based as

Язык: Английский

Процитировано

0
Membrane fluidity control by the Magnaporthe oryzae acyl-CoA binding protein sets the thermal range for host rice cell colonization DOI Creative Commons
Michael Richter, Lauren Segal, Raquel O. Rocha

и другие.

PLoS Pathogens, Год журнала: 2024, Номер 20(11), С. e1012738 - e1012738

Опубликована: Ноя. 25, 2024

Following leaf cuticle penetration by specialized appressorial cells, the devastating blast fungus Magnaporthe oryzae grows as invasive hyphae (IH) in living rice cells. IH are separated from host cytoplasm plant-derived membranes forming an apoplastic compartment and a punctate biotrophic interfacial complex (BIC) that mediate molecular host-pathogen interaction. What cellular processes determine temperature range for this growth stage is unanswered question pertinent to broader understanding of how phytopathogens may cope with environmental stresses arising under climate change. Here, we shed light on thermal adaptation M. disrupting ACB1 gene encoding single acyl-CoA-binding protein, intracellular transporter long-chain acyl-CoA esters. Loss affected fatty acid desaturation levels abolished pathogenicity at optimal (26°C) low (22°C) but not elevated (29°C) infection temperatures (the latter following post-penetration shifts 26°C). Relative wild type, Δacb1 mutant strain exhibited poor vegetative impaired membrane trafficking 22°C 26°C, 29°C. In planta, was inhibited 26°C-which accompanied multi-BIC phenotype-but 29°C, where BIC formation normal. Underpinning phenotype fluidity 26°C temperatures, indicating Acb1 suppresses rigidity optimal- suboptimal- supraoptimal temperatures. Deducing temperature-dependent role maintaining homeostasis reveals disease both mechanistically determined wider than hitherto appreciated.

Язык: Английский

Процитировано

0