A Necrotizing Toxin Promotes Pseudomonas syringae Infection Across Evolutionarily Divergent Plant Lineages DOI

Kristina Grenz,

Khong‐Sam Chia, Emma K Turley

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 19, 2024

ABSTRACT The Pseudomonas syringae species complex harbors a diverse range of plant pathogenic bacteria. While much the current understanding P. is centered on interactions with flowering plants, less known about infection in evolutionarily divergent non-flowering lineages. Here, we took comparative evolutionary approach to understand how infects distantly related plants. We identify broad host isolates causing significant disease liverwort Marchantia polymorpha , fern Ceratopteris richardii and Nicotiana benthamiana which last shared common ancestor over 500 million years ago. demonstrate that phytotoxin enriched belonging phylogroup 2 clade are particularly virulent relying combination type-3 secreted effector proteins lipopeptide syringomycin. application purified syringomycin promotes necrosis tissues activates conserved genes associated redox regulation cell death. Toxin-deficient phylogroups normally unable infect thalli exhibit enhanced bacterial growth when supplemented exogenous syringomycin, further highlighting its role as host-range defining factor . Collectively our research reveals key for promoting colonization, works concert antagonize an exceptionally wide spectrum land

Язык: Английский

Genetic dissection of the tissue‐specific roles of type III effectors and phytotoxins in the pathogenicity of Pseudomonas syringae pv. syringae to cherry DOI Creative Commons
Andrea Vadillo‐Dieguez, Ziyue Zeng, John W. Mansfıeld

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(4)

Опубликована: Апрель 1, 2024

Abstract When compared with other phylogroups (PGs) of the Pseudomonas syringae species complex, P. pv. s yringae (Pss) strains within PG2 have a reduced repertoire type III effectors (T3Es) but produce several phytotoxins. Effectors cherry pathogen Pss 9644 were grouped based on their frequency in from Prunus as conserved effector locus (CEL) common to most pathogens; core PG2; set PRUNUS and FLEXIBLE T3Es. also contains gene clusters for biosynthesis toxins syringomycin, syringopeptin syringolin A. After confirmation virulence expression, mutants sequential series T3E toxin deletions pathogenicity tested wood, leaves fruits sweet ( avium ) ornamental incisa ). The had key role disease development less important wood. An effectorless mutant retained some fruit not wood or leaves. Striking redundancy was observed amongst groups. CEL roles during early stages leaf infection possibly acted synergistically all tissues. Deletion separate groups T3Es more effect than . Mixed inocula used complement mutations trans indicated that strain mixtures may be field. Our results highlight niche‐specific tissues complexity 9644.

Язык: Английский

Процитировано

6

A necrotizing toxin enables Pseudomonas syringae infection across evolutionarily divergent plants DOI Creative Commons

Kristina Grenz,

Khong‐Sam Chia, Emma K Turley

и другие.

Cell Host & Microbe, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

3

Exploring the open (host) range DOI
David A. Baltrus, Brian H. Kvitko

Cell Host & Microbe, Год журнала: 2025, Номер 33(1), С. 10 - 12

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Global Insights into Bacterial Canker of Stone Fruits Caused by the Pseudomonas Syringae Species Complex DOI
Andrea Vadillo‐Dieguez, Robert W. Jackson, Michelle T. Hulin

и другие.

Plant Health Cases, Год журнала: 2025, Номер unknown

Опубликована: Май 2, 2025

Abstract Stone fruits ( Prunus spp.) are economically and nutritionally valuable commodities, with production concentrated in the temperate regions. In UK, limited to cherries plums, trends highlight a resurgence cherry yields due modern practices. However, bacterial cankers, caused by diverse strains of P . syringae , pose significant threats spp., affecting both commercial orchards ornamental plant nurseries. This disease, characterised gummosis, necrotic lesions woody tissues, incurs severe economic losses, young particularly vulnerable. Surveys have revealed emergence novel pathogens populations, predominant groups varying across continents. Advances molecular techniques improved pathogen classification resistance screening cultivars is increasing. Addressing cankers requires integrating knowledge lifecycles, virulence factors, host-pathogen interactions into management strategies. Current research focuses on evolution, breeding resistant tree cultivars, implementing new biocontrol strategies mitigate ecological impacts. Information © The Authors 2025

Язык: Английский

Процитировано

0

Pathogenicity, phylogenomic, and comparative genomic study of Pseudomonas syringae sensu lato affecting sweet cherry in California DOI Creative Commons
Tawanda E. Maguvu,

Rosa J. Frias,

Alejandro I. Hernandez-Rosas

и другие.

Microbiology Spectrum, Год журнала: 2024, Номер 12(10)

Опубликована: Сен. 3, 2024

To gain insights into the diversity of

Язык: Английский

Процитировано

1

A Necrotizing Toxin Promotes Pseudomonas syringae Infection Across Evolutionarily Divergent Plant Lineages DOI

Kristina Grenz,

Khong‐Sam Chia, Emma K Turley

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 19, 2024

ABSTRACT The Pseudomonas syringae species complex harbors a diverse range of plant pathogenic bacteria. While much the current understanding P. is centered on interactions with flowering plants, less known about infection in evolutionarily divergent non-flowering lineages. Here, we took comparative evolutionary approach to understand how infects distantly related plants. We identify broad host isolates causing significant disease liverwort Marchantia polymorpha , fern Ceratopteris richardii and Nicotiana benthamiana which last shared common ancestor over 500 million years ago. demonstrate that phytotoxin enriched belonging phylogroup 2 clade are particularly virulent relying combination type-3 secreted effector proteins lipopeptide syringomycin. application purified syringomycin promotes necrosis tissues activates conserved genes associated redox regulation cell death. Toxin-deficient phylogroups normally unable infect thalli exhibit enhanced bacterial growth when supplemented exogenous syringomycin, further highlighting its role as host-range defining factor . Collectively our research reveals key for promoting colonization, works concert antagonize an exceptionally wide spectrum land

Язык: Английский

Процитировано

0