Major genomic regions responsible for wheat yield and its components as revealed by meta-QTL and genotype–phenotype association analyses

Planta, Год журнала: 2020, Номер 252(4)

Опубликована: Сен. 24, 2020

Язык: Английский

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Genome-wide identification of MYB genes and expression analysis under different biotic and abiotic stresses in Helianthus annuus L.

Industrial Crops and Products, Год журнала: 2019, Номер 143, С. 111924 - 111924

Опубликована: Ноя. 15, 2019

Язык: Английский

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Ectopic expression of Triticum polonicum VRT-A2 underlies elongated glumes and grains in hexaploid wheat in a dosage-dependent manner

The Plant Cell, Год журнала: 2021, Номер 33(7), С. 2296 - 2319

Опубликована: Апрель 29, 2021

Abstract Flower development is an important determinant of grain yield in crops. In wheat (Triticum spp.), natural variation for the size spikelet and floral organs particularly evident Triticum turgidum ssp. polonicum (also termed polonicum), a tetraploid subspecies with long glumes, lemmas, grains. Using map-based cloning, we identified VEGETATIVE TO REPRODUCTIVE TRANSITION 2 (VRT2), which encodes MADS-box transcription factor belonging to SHORT PHASE family, as gene underlying T. long-glume (P1) locus. The causal P1 mutation sequence rearrangement intron-1 that results ectopic expression VRT-A2 allele. Based on allelic studies, propose unique subspecies-defining polymorphism, was later introduced into hexaploid via hybridizations. Near-isogenic lines differing locus revealed gradient effect across spikelets within florets. Transgenic carrying allele show levels are highly correlated spike, glume, grain, organ length. These highlight how changes profiles, through cis-regulation, can affect agronomic traits dosage-dependent manner polyploid

Язык: Английский

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Genome-wide identification and expression analysis of bHLH transcription factor family in response to cold stress in sweet cherry (Prunus avium L.)

Scientia Horticulturae, Год журнала: 2021, Номер 279, С. 109905 - 109905

Опубликована: Янв. 18, 2021

Язык: Английский

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Integrative iTRAQ-based proteomic and transcriptomic analysis reveals the accumulation patterns of key metabolites associated with oil quality during seed ripening of Camellia oleifera

Horticulture Research, Год журнала: 2021, Номер 8(1)

Опубликована: Июль 1, 2021

Camellia oleifera (C. oleifera) is one of the four major woody oil-bearing crops in world and has relatively high ecological, economic, medicinal value. Its seeds undergo a series complex physiological biochemical changes during ripening, which mainly manifested as accumulation transformation certain metabolites closely related to oil quality, especially flavonoids fatty acids. To obtain new insights into underlying molecular mechanisms, parallel analysis transcriptome proteome profiles C. at different maturity levels was conducted using RNA sequencing (RNA-seq) isobaric tags for relative absolute quantification (iTRAQ) complemented with gas chromatography-mass spectrometry (GC-MS) data. A total 16,530 transcripts 1228 proteins were recognized significant differential abundances pairwise comparisons samples various developmental stages. Among these, 317 coexpressed poor correlation, most involved metabolic processes, including acid metabolism, α-linolenic glutathione metabolism. In addition, content decreased gradually seed maturity, acids generally peaked fat stage; these results basically agreed regulation patterns genes or corresponding pathways. The expression annotated upstream candidates phenylalanine ammonia-lyase (PAL) chalcone synthase (CHS) well their cognate positively correlated variation flavonoid content, while shikimate O-hydroxycinnamoyltransferase (HCT)-encoding had opposite pattern. increase abundance mRNAs alcohol dehydrogenase (ADH) associated reduction linoleic synthesis. Using weighted gene coexpression network (WGCNA), we further identified six unique modules flavonoid, oil, anabolism that contained hub similar transcription factors (TFs), such MADS intervening keratin-like C-terminal (MIKC_MADS), type-B authentic response regulator (ARR-B), basic helix-loop-helix (bHLH). Finally, based on known pathways WGCNA combined correlation analysis, five composed cinnamyl-alcohol dehydrogenases (CADs), caffeic 3-O-methyltransferase (COMT), flavonol (FLS), 4-coumarate: CoA ligase (4CL) screened out. With this exploratory multiomics dataset, our presented dynamic picture regarding maturation process Hainan Island, not only revealing temporal specific key candidate but also providing scientific basis genetic improvement tree species.

Язык: Английский

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Genetic basis of geographical differentiation and breeding selection for wheat plant architecture traits

Genome biology, Год журнала: 2023, Номер 24(1)

Опубликована: Май 12, 2023

Abstract Background Plant architecture associated with increased grain yield and adaptation to the local environments is selected during wheat ( Triticum aestivum ) breeding. The internode length of individual stems tiller plants are important for determination plant architecture. However, few studies have explored genetic basis these traits. Results Here, we conduct a genome-wide association study (GWAS) dissect geographical differentiation traits in 306 worldwide accessions including both landraces traditional varieties. We determine changes haplotypes genomic regions frequency 831 that either introduced from other countries or developed China last two decades. identify 83 loci one trait, while remaining 247 pleiotropic. also find 163 under strong selective sweep. GWAS results demonstrate independent regulation consistent plants. This makes it possible obtain ideal haplotype combinations four internodes. distribution explains observed differences among accessions. Conclusion provides insights into It will facilitate gene functional analysis molecular design

Язык: Английский

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Genome-wide identification and expression analysis of the Auxin-Response factor (ARF) gene family in Medicago sativa under abiotic stress

BMC Genomics, Год журнала: 2023, Номер 24(1)

Опубликована: Авг. 29, 2023

Abstract Background Alfalfa ( Medicago sativa ) is the most widely planted legume forage and one of economically valuable crops in world. The periodic changes its growth development abiotic stress determine yield economic benefits. Auxin controls many aspects alfalfa by regulating gene expression, including organ differentiation response. response factors (ARF) are transcription that play an essential role auxin signal transduction regulate expression auxin-responsive genes. However, function ARF unclear autotetraploid-cultivated alfalfa. Result A total 81 were identified genome this study. Gene Ontology (GO) terms Kyoto Encyclopedia Genes Genomes (KEGG) pathways analyzed, identifying genes mainly involved transcriptional regulation plant hormone pathways. Phylogenetic analysis showed MsARF was divided into four clades: I, II, III, IV, each containing 52, 13, 7, 9 genes, respectively. promoter region contained stress-related elements, such as ABRE, TC-rich repeats, MBS, LTR. Proteins encoded 50 localized nucleus without guide peptides, or transmembrane structures, indicating not secreted transported but only nucleus. Protein structure revealed secondary tertiary structures varied. Chromosomal localization unevenly distributed on 25 chromosomes, with highest distribution chromosome 5. Furthermore, 14 segmental duplications two sets tandem repeats identified. Expression indicated differentially expressed different tissues under various stressors. quantitative reverse polymerase chain reaction (qRT-PCR) profiles 23 specific to stresses drought, salt, high temperature, low well tissue-specific closely related duration stress. Conclusion This study cultivated based autotetraploid level, which GO, KEGG analysis, phylogenetic sequence characteristics, pattern further confirmed. Together, these findings provide clues for investigation functional verification molecular breeding provides a novel approach systematically identify characterize alfalfa, revealing significantly stresses.

Язык: Английский

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TaMADS29 interacts with TaNF-YB1 to synergistically regulate early grain development in bread wheat

Science China Life Sciences, Год журнала: 2023, Номер 66(7), С. 1647 - 1664

Опубликована: Фев. 17, 2023

Язык: Английский

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A high-resolution genotype–phenotype map identifies the TaSPL17 controlling grain number and size in wheat

Genome biology, Год журнала: 2023, Номер 24(1)

Опубликована: Авг. 28, 2023

Large-scale genotype-phenotype association studies of crop germplasm are important for identifying alleles associated with favorable traits. The limited number single-nucleotide polymorphisms (SNPs) in most wheat genome-wide (GWASs) restricts their power to detect marker-trait associations. Additionally, only a few genes regulating grain per spikelet have been reported due sensitivity this trait variable environments.We perform large-scale GWAS using approximately 40 million filtered SNPs 27 spike morphology We 132,086 significant associations and the SNP markers located within 590 peaks. additional stronger peaks by dividing into sub-traits relative results propose that genetic dissection is powerful strategy signals yield traits wheat. reveal TaSPL17 positively controls size floret meristem development, which turn leads enhanced plant. haplotypes at indicate geographical differentiation, domestication effects, breeding selection.Our study provides valuable resources improvement fast-forward solution candidate gene detection cloning

Язык: Английский

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Photoperiod-1 regulates the wheat inflorescence transcriptome to influence spikelet architecture and flowering time

Current Biology, Год журнала: 2024, Номер 34(11), С. 2330 - 2343.e4

Опубликована: Май 22, 2024

Photoperiod insensitivity has been selected by breeders to help adapt crops diverse environments and farming practices. In wheat, insensitive alleles of Photoperiod-1 (Ppd-1) relieve the requirement long daylengths flower promoting expression floral genes early in season; however, these also limit yield reducing number fertility grain-producing florets through processes that are poorly understood. Here, we performed transcriptome analysis developing inflorescence using near-isogenic lines contain either photoperiod-insensitive or null Ppd-1, during stages when spikelet is determined floret development initiates. We report Ppd-1 influences stage-specific with roles auxin signaling, meristem identity, protein turnover, differentially expressed transcripts identified bZIP ALOG transcription factors, namely PDB1 ALOG1, which regulate flowering time architecture. These findings enhance our understanding introduce new targets for improving potential.

Язык: Английский

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