Xylem sap residue in cut-open conduits can affect gas discharge in pneumatic experiments DOI Creative Commons
Marcela T. Miranda, Luciano Pereira, Gabriel S. Pires

и другие.

Tree Physiology, Год журнала: 2024, Номер unknown

Опубликована: Июль 24, 2024

Abstract Considerable attention has been paid to addressing methodological concerns related measurements of embolism in conduits angiosperm xylem. A fast, easy and cheap method is based on gas extraction from dehydrating samples obtain pneumatic vulnerability curves (VCs). Here, we tested the assumption that cutting open leads gas-filled lumina when these are cut air at fairly high water potentials, which required detect intact conduits. We performed VCs with Pneumatron for 12 species extracted sap cut-open vessels branches nine under early stages branch dehydration. The optical was applied Citrus plants as an alternative reference estimate resistance. found increase discharge during dehydration, affected most studied. Xylem residue not absorbed immediately by surrounding tissue six but gradually disappeared over time progressive amount discharged increased until all residual absorbed, embolism. conclude xylem affects represents a novel artifact can easily be corrected for. Yet, it remains unclear why exactly air–water meniscus did fully withdraw conduit end wall species. By analyzing slope time, could improve estimations resistance, evidenced strong agreement between methods. Since some slightly underestimate propose apply correction this time-resolution taken Pneumatron.

Язык: Английский

Xylem sap residue in cut-open conduits can affect gas discharge in pneumatic experiments DOI Creative Commons
Marcela T. Miranda, Luciano Pereira, Gabriel S. Pires

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 9, 2023

Summary Considerable progress has been made in understanding the mechanisms of embolism formation based on pneumatic method, which relies gas discharge measurements. Here, we test assumption that cut-open conduits are gas-filled when samples cut at high water potentials. We performed vulnerability curves (VC) with Pneumatron and analysed sap extraction from vessels Citrus branches, while optical method was applied as a reference method. VCs 11 additional angiosperms were to generalise our findings. found an increase during early stages dehydration, affected VC . Xylem not absorbed immediately by surrounding tissue branches. The amount discharged increased until all residue absorbed, near turgor loss point. By analysing slope VCs, could correct VC, evidenced strong agreement resistance between Since residual some species slightly reduce species, propose apply easy correction for this novel artefact. Automated measurements also required because its time resolution.

Язык: Английский

Процитировано

3
Further Test of Pneumatic Method in Constructing Vulnerability Curves Using Six Tree Species with Contrasting Xylem Anatomy DOI Open Access
Han Zhao, Yueyang Li,

Su‐Hui Liao

и другие.

Forests, Год журнала: 2023, Номер 14(2), С. 293 - 293

Опубликована: Фев. 2, 2023

The pneumatic method is a novel determining vulnerability to embolism in plants, yet it remains unclear whether this suitable for all species with different xylem anatomy. In study, using six tree contrasting anatomy, including four vessel-bearing (diffuse-porous wood and ring-porous wood) two tracheid-bearing (non-porous wood), we test the reliability of by comparing hydraulic methods also considering turgor loss point native embolism. Vessel length distribution cut-open vessel volume were evaluated silicone injection technique. Additionally, synthesized published data find out consistency between methods. Results showed that there was maximum 10-folds difference mean diameter varying from 30 56 μm among species. estimated open ranges 0.064 0.397 mL, 14% tube vacuum reservoir. For species, good methods, evidenced significantly overestimated because bad consistencies plant water relations. Data synthesis suggested can accurately measure but not Our study provided further evidence accurate most thus has potential be widely used hydraulics field. However, proposed precise calculation air discharge should take into account vessels wide long vessels.

Язык: Английский

Процитировано

2
Xylem sap residue in cut-open conduits can affect gas discharge in pneumatic experiments DOI Creative Commons
Marcela T. Miranda, Luciano Pereira, Gabriel S. Pires

и другие.

Tree Physiology, Год журнала: 2024, Номер unknown

Опубликована: Июль 24, 2024

Abstract Considerable attention has been paid to addressing methodological concerns related measurements of embolism in conduits angiosperm xylem. A fast, easy and cheap method is based on gas extraction from dehydrating samples obtain pneumatic vulnerability curves (VCs). Here, we tested the assumption that cutting open leads gas-filled lumina when these are cut air at fairly high water potentials, which required detect intact conduits. We performed VCs with Pneumatron for 12 species extracted sap cut-open vessels branches nine under early stages branch dehydration. The optical was applied Citrus plants as an alternative reference estimate resistance. found increase discharge during dehydration, affected most studied. Xylem residue not absorbed immediately by surrounding tissue six but gradually disappeared over time progressive amount discharged increased until all residual absorbed, embolism. conclude xylem affects represents a novel artifact can easily be corrected for. Yet, it remains unclear why exactly air–water meniscus did fully withdraw conduit end wall species. By analyzing slope time, could improve estimations resistance, evidenced strong agreement between methods. Since some slightly underestimate propose apply correction this time-resolution taken Pneumatron.

Язык: Английский

Процитировано

0