Crop Protection, Год журнала: 2024, Номер unknown, С. 106987 - 106987
Опубликована: Окт. 1, 2024
Язык: Английский
Cell Reports, Год журнала: 2024, Номер 43(3), С. 113838 - 113838
Опубликована: Фев. 21, 2024
Lysine acetylation is a dynamic post-translational modification of proteins. Extensive studies have revealed that the modulated by histone acetyltransferases and deacetylases (HDACs) plays crucial role in regulating protein function. However, there has been limited focus on how HDACs regulate jasmonic acid (JA) biosynthesis plants. Here, we uncover stability OsLOX14, critical enzyme involved JA biosynthesis, regulated deacetylase, OsHDA706, hindered viral protein. Our results show OsHDA706 deacetylates OsLOX14 enhances leading to accumulation an improved broad-spectrum rice antiviral defense. Furthermore, found P2, encoded destructive stripe virus, disrupts association OsHDA706-OsLOX14, promoting infection. Overall, our findings reveal HDAC manipulates interplay deacetylation biosynthetic enhance plant responses.
Язык: Английский
Процитировано
10
npj Biofilms and Microbiomes, Год журнала: 2024, Номер 10(1)
Опубликована: Июль 30, 2024
Abstract Plant-sucking insects have intricate associations with a diverse array of microorganisms to facilitate their adaptation specific ecological niches. The midgut phytophagous true bugs is generally structured into four distinct compartments accommodate microbiota. Nevertheless, there limited understanding regarding the origins these gut microbiomes, mechanisms behind microbial community assembly, and interactions between microbiomes insect hosts. In this study, we conducted comprehensive survey communities within bean bug Riptortus pedestris , soybean plant, bulk soil across 12 geographical fields in China, utilizing high-throughput sequencing 16 S rRNA gene. Our findings illuminated that microbiota plant-sucking predominantly originated from surrounding environment, plants also play subordinate role mediating acquisition for insects. Furthermore, our investigation suggested composition microbiome was probably shaped by host selection and/or microbe-microbe at compartment level, marginal influence factors. Additionally, had unveiled noteworthy dynamic core bacterial taxa, particularly Burkholderia which were initially sourced environment subsequently enriched compartments. This enrichment played significant enhancing reproduction. These contribute evolving insect-plant-soil ecosystem, shedding additional light on underpin significance partnerships adaptation.
Язык: Английский
Процитировано
10
Journal of Applied Entomology, Год журнала: 2023, Номер 147(6), С. 406 - 415
Опубликована: Апрель 15, 2023
Abstract Organophosphate insecticides have been widely used in agriculture to control insect pests and raised growing problems including insecticide resistance. According recent studies, microbial symbionts play important roles developing The bean bug Riptortus pedestris is a notorious pest of leguminous crops, harbouring diverse microorganisms their midguts. However, the potential biological many gut‐associated bacteria are largely unknown. In this study, we isolated bacterial strain from midgut R. identified it as Serratia marcescens based on its morphological characteristics phylogenetic analysis 16S rRNA gene sequence. A halo phosphorus‐solubilizing was observed medium containing lecithin an organophosphorus dimethoate. Subsequently, degradation dimethoate determined using Gas Chromatography with Mass Spectroscopy (GC–MS). Further indicated that S. contained organophosphorus‐degrading MBL‐fold metallo‐hydrolase gene. Additionally, discovered bacterium could colonize stably enhance host survivorship when exposed Our results revealed degrade compounds, thereby reducing related toxicity hosts. These findings provided further insights into host‐microbiota interactions underpinning development
Язык: Английский
Процитировано
15
Molecular Plant Pathology, Год журнала: 2023, Номер 24(6), С. 560 - 569
Опубликована: Март 14, 2023
The bean bug (Riptortus pedestris), one of the most important pests soybean, causes staygreen syndrome, delaying plant maturation and affecting pod development, resulting in severe crop yield loss. However, little is known about underlying mechanism this pest. In study, we found that a salivary secretory protein, Rp614, induced cell death nonhost Nicotiana benthamiana leaves. NbSGT1 NbNDR1 are involved Rp614-induced death. Tissue specificity analysis showed Rp614 mainly present glands highly during pest feeding. RNA interference experiments syndrome caused by R. pedestris was significantly attenuated when silenced. Together, our results indicate plays an essential role infestation provide promising target for control.
Язык: Английский
Процитировано
12
Insect Science, Год журнала: 2024, Номер unknown
Опубликована: Сен. 18, 2024
Hemiptera is one of the most significant orders insect pests, including whiteflies, true bugs, aphids, planthoppers, psyllids, and so forth, which have led to substantial economic losses in agricultural industries significantly affected food yields through their ability suck phloem sap crops transmit numerous bacterial viral pathogens. Therefore, explorations pest-specific, eco-friendly easy-to-adopt technologies for hemipteran pest control are urgently needed. To best our knowledge, microRNAs (miRNAs), endogenous non-coding small RNAs approximately 22 nucleotides length, involved regulating gene expression via direct recognition binding 3'-untranslated region (3'-UTR) target messenger (mRNAs) or by acting as a center competitive RNA (ceRNA) network at post-transcriptional level. This review systematically outlines characterization functional investigation miRNA biogenesis pathway such aphids planthoppers. In addition, we explored results sequencing observations miRNAs these suggest that obtained annotated high-throughput technology bioinformatic analyses contribute molting development, fitness, wing polyphenism, symbiont interactions insecticide resistance pests. Finally, summarize current advances propose framework future research extend data address potential limitations application miRNAs.
Язык: Английский
Процитировано
4
Phytopathology Research, Год журнала: 2024, Номер 6(1)
Опубликована: Сен. 20, 2024
Abstract Oligosaccharins, widely recognized as plant immunity inducers, have been applied and studied for enhancing antiviral defenses in rice over the years. However, due to complex induction mechanism rice, further studies are needed. In this study, we demonstrated that oligosaccharins enhanced resistance southern black-streaked dwarf virus (SRBSDV) at both symptomatic molecular levels. RNA-seq analysis revealed defense by activating multiple pathways. Among them, jasmonic acid (JA) signaling pathway, a crucial pathway was investigated detail. Interestingly, data showed genes related JA were significantly induced after 1 h but not 24 of treatment, suggesting rapid sensitive. Furthermore, levels sensitivity accumulation, making treated plants more sensitive methyl jasmonate (MeJA). Additionally, treatment also burst reactive oxygen species (ROS) rice. Notably, low-dose effect, within certain dilution range from 6000-fold 1000-fold diluted solution 5% oligosaccharins, with 4000-fold exhibiting stronger ability induce elevated production ROS. Moreover, application SRBSDV. These suggest can enhance inducing pathways, including ROS production.
Язык: Английский
Процитировано
3
Frontiers in Microbiology, Год журнала: 2025, Номер 16
Опубликована: Янв. 22, 2025
Introduction Riptortus pedestris is a destructive pest that threatens multiple leguminous crops in China. The intestinal microbiota plays crucial role the growth and reproduction of host insects. However, composition function gut at different developmental stages remain unclear. Methods Here, metagenomic sequencing was performed to clarify microbial diversity 2nd-, 3rd-, 4th-, 5th- instar nymphs (2 N–5 N) female adults (FAs) R. effects vital bacteria on development detected. have stage specificity, indicating their . Results Enterococcus Caballerronia were predominant present during 2 N–FAs. In addition, abundances 3 N 4 guts significantly greater than those others guts. Furthermore, 5 harbored abundant Burkholderia - Paraburkholderia Caballeronia metabolic pathways enriched from FAs. Carbohydrate metabolism, including glycoside hydrolases (GHs) glycosyl transferases (GTs), occurs throughout entire stage. Many antibiotic resistance genes (ARGs) detected Pseudomonadota Bacillota presented broad spectrum resistance. Excitingly, eliminated by treatment unable molt normally, lifespan shortened nymphs, had significant effect nymph development. Conclusion summary, our results, for first time, systematically illustrate abundance across demonstrate genera are This study provides basis stinkbug management strategies focus pivotal microbiota.
Язык: Английский
Процитировано
0
Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(5)
Опубликована: Янв. 31, 2025
The stinkbug Riptortus pedestris , notorious for inducing soybean staygreen-like syndrome, employs a range of salivary proteins to manipulate the host plant its benefit. Here, we show that RpSP1, protein specific Pentatomomorpha, triggers defense responses in multiple species. RpSP1 interacts with and stabilizes HSP40 family GmSPIP1 is dependent on induce cell death. We critical 22-amino acid peptide within acts as an intracellular insect-derived elicitor. Furthermore, enhances insect-feeding efficiency. dual functionality highlighted by significant reduction syndrome following overexpression plants or knockdown insects. Our findings elucidate complex molecular interactions between herbivores, positioning crucial target developing advanced pest management strategies broad implications agricultural biology.
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 1882 - 1882
Опубликована: Фев. 22, 2025
Serine acetyltransferase (SAT) is a critical enzyme in the sulfur-assimilation pathway of cysteine, playing an essential role numerous physiological functions plants, particularly their response to environmental stresses. However, structural characteristics soybean SAT gene family remain poorly understood. Members were identified using Hidden Markov Model approach. Bioinformatics tools, such as ExPASy, PlantCARE, MEME, and TBtools-II, employed examine physicochemical properties, cis-regulatory elements, conserved motifs, structures, chromosomal positions GmSAT genes. RT-qPCR was conducted evaluate expression profiles genes under NaCl-induced stress, identifying likely involved salt-stress response. A total ten genome grouped into three subfamilies. Genes within each subfamily shared notable similarities motifs. Analysis elements revealed that promoters these contain several linked plant growth stress-related responses. Expression patterns varied across different tissues, with GmSAT10 showing higher roots, while GmSAT1 GmSAT2 had lower same tissue. Following NaCl treatment, levels seven significantly increased indicating potential involvement plant's adaptation salt stress. appear play crucial roles soybean's offering insights could aid development salt-tolerant varieties.
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Март 10, 2025
Stay-green syndrome (SGS) resistant germplasms serve as the cornerstone for soybean improvement. A comprehensive assessment was conducted on a panel of 1553 to evaluate their resistance SGS through natural inoculation. Over three-year period, one landrace, ZaoShuHeiDou, emerged resilient SGS, displaying significantly reduced risk (p < 0.05) compared its counterparts. The broad-sense heritability 0.86. Notably, landraces demonstrated substantially lower incidence improved cultivars. Furthermore, severity correlated with several key traits pertinent improvement efforts. In field trials, application insecticides effectively mitigated severity, whereas use organic fertilizers and soil sterilization had no discernible impact. total 83 DNA samples were successfully amplified from SGS-afflicted plants using specific primers stay-green associated virus (SoSGV), SoSGV LCU-1 strain isolated. qPCR analysis confirmed positive correlation between relative copy number in afflicted plants. findings this study lay solid foundation development SGS-resistant cultivars exploration underlying mechanisms.
Язык: Английский
Процитировано
0