The MYC2–PUB22–JAZ4 module plays a crucial role in jasmonate signaling in tomato

Molecular Plant, Год журнала: 2024, Номер 17(4), С. 598 - 613

Опубликована: Фев. 9, 2024

Jasmonates (JAs), a class of lipid-derived stress hormones, play crucial role across an array plant physiological processes and responses. Although JA signaling is thought to rely predominantly on the degradation specific JAZ proteins by SCFCOI1, it remains unclear whether other pathways are involved in regulation protein stability. Here, we report that PUB22, U-box type E3 ubiquitin ligase, plays critical resistance against Helicoverpa armigera responses tomato. Whereas COI1 physically interacts with JAZ1/2/5/7, PUB22 JAZ1/3/4/6. ubiquitinates JAZ4 promote its via 26S proteasome pathway. Importantly, observed pub22 mutants showreduced H. armigera, whereas jaz4 single jaz1 jaz3 jaz6 quadruple have enhanced resistance. The hypersensitivity herbivores could be partially rescued mutation. Moreover, found expression can transcriptionally activated MYC2, thus forming positive feedback circuit signaling. We noticed PUB22–JAZ4 module also regulates responses, including defense B. cinerea, inhibition root elongation, anthocyanin accumulation. Taken together, these results indicate growth together COI1-regulated signaling, targeting JAZs.

Язык: Английский

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OsNAC5 orchestrates OsABI5 to fine‐tune cold tolerance in rice

Journal of Integrative Plant Biology, Год журнала: 2023, Номер 66(4), С. 660 - 682

Опубликована: Ноя. 16, 2023

Due to its tropical origins, rice (Oryza sativa) is susceptible cold stress, which poses severe threats production. OsNAC5, a NAC-type transcription factor, participates in the stress response of rice, but detailed mechanisms remain poorly understood. Here, we demonstrate that OsNAC5 positively regulates tolerance at germination and seedlings by directly activating expression ABSCISIC ACID INSENSITIVE 5 (OsABI5). Haplotype analysis indicated single nucleotide polymorphisms NAC-binding site OsABI5 promoter are strongly associated with tolerance. also enhanced stability, thus regulating cold-responsive (COR) genes, enabling fine-tuned control action for rapid, precise plant responses stress. DNA affinity purification sequencing coupled transcriptome deep identified several target genes involved COR expression, including DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR 1A (OsDREB1A), OsMYB20, PEROXIDASE 70 (OsPRX70). In vivo vitro analyses suggested gene transcription, marked upregulation OsNAC5-overexpressing lines downregulation osnac5 and/or osabi5 knockout mutants. This study extends our understanding regulation via through OsABI5-CORs module, may be used ameliorate advanced breeding.

Язык: Английский

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ABI5 binding proteins: key players in coordinating plant growth and development

Trends in Plant Science, Год журнала: 2024, Номер 29(9), С. 1006 - 1017

Опубликована: Апрель 6, 2024

During the course of terrestrial evolution, plants have developed complex networks that involve coordination phytohormone signalling pathways in order to adapt an ever-changing environment. Transcription factors coordinate these responses by engaging different protein complexes and exerting both positive negative effects. ABA INSENSITIVE 5 (ABI5) binding proteins (AFPs), which are closely related NOVEL INTERACTOR OF JAZ (NINJA)-like proteins, known for their fundamental role plants' morphological physiological growth. Recent studies shown AFPs regulate several hormone-signalling pathways, including abscisic acid (ABA) gibberellic (GA). Here, we review genetic control crosstalk with plant hormone signalling, discuss contributions growth development.

Язык: Английский

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Interplay of light and abscisic acid signaling to modulate plant development

Journal of Experimental Botany, Год журнала: 2024, Номер 76(3), С. 730 - 745

Опубликована: Апрель 25, 2024

Exogenous light cues and the phytohormone abscisic acid (ABA) regulate several aspects of plant growth development. In recent years, role crosstalk between ABA signaling pathways in regulating different physiological processes has become increasingly evident. This includes regulation germination early seedling development, control stomatal development conductance, growth, roots, buds, branches, flowering. Light cascades have various convergence points at both DNA protein levels. The molecular involves factors such as HY5, COP1, PIFs, BBXs that integrate with components PYL receptors ABI5. particular, ABI5 PIF4 promoters are key 'hotspots' for integrating these two pathways. Plants acquired before they colonized land almost 500 million years ago. this review, we discuss advances interplay provide an overview evolution

Язык: Английский

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Pectin methylesterase 31 is transcriptionally repressed by ABI5 to negatively regulate ABA-mediated inhibition of seed germination

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Фев. 2, 2024

Pectin methylesterase (PME), a family of enzymes that catalyze the demethylation pectin, influences seed germination. Phytohormone abscisic acid (ABA) inhibits However, little is known about function PMEs in response to ABA-mediated In this study, we found role PME31 inhibition The expression prominent embryo and repressed by ABA treatment. Phenotype analysis showed disruption increases germination, whereas overexpression attenuates effect. Further study ABI5, an signaling bZIP transcription factor, identified as upstream regulator PME31. Genetic functions downstream ABI5 Detailed studies directly binds promoter its expression. plants, reduced Taken together, transcriptionally inhibited negatively regulates germination inhibition. These findings shed new light on mechanisms

Язык: Английский

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REPRESSOR OF UV-B PHOTOMORPHOGENESIS proteins target ABA INSENSITIVE 5 for degradation to promote early plant development

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 196(4), С. 2490 - 2503

Опубликована: Авг. 28, 2024

REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 (RUP1) and 2 (RUP2) are WD-40 domain-containing proteins that have been extensively characterized for their role in signaling. However, the roles of RUP outside canonical UV-signaling pathway less known. Here, we identify RUP1 RUP2 play important ABA signaling to regulate seed germination early seedling development Arabidopsis thaliana. Our protein interaction studies confirmed physically interact with INSENSITIVE 5 (ABI5). In presence abscisic acid, rup1, rup2, rup1rup2 exhibited reduced establishment compared wild type. Germination rup1rup2abi5-8 were similar abi5-8, suggesting suppress ABA-mediated inhibition an ABI5-dependent manner. The DDB1-binding WD40 promoted ubiquitination ABI5 its degradation. ABI5, turn, establishes a negative feedback loop inhibit expression RUP1/RUP2. also inhibited direct binding ELONGATED HYPOCOTYL (HY5) promoters under ABA. This study highlights coordinated action RUP1, RUP2, HY5 regulating plant development.

Язык: Английский

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ABSCISIC ACID INSENSITIVE 3 promotes auxin signalling by regulating SHY2 expression to control primary root growth in response to dehydration stress

Journal of Experimental Botany, Год журнала: 2024, Номер 75(16), С. 5111 - 5129

Опубликована: Май 20, 2024

Plants combat dehydration stress through different strategies including root architectural changes. Here we show that when exposed to varying levels of stress, primary growth in Arabidopsis is modulated by regulating meristem activity. Abscisic acid (ABA) concert with auxin signalling adjust according levels. ABSCISIC ACID INSENSITIVE 3 (ABI3), an ABA-responsive transcription factor, stands at the intersection ABA and fine-tunes response stress. Under low or induction ABI3 expression promotes decreasing SHY2, a negative regulator response. This further enhances transporter gene PIN1 cell cycle CYCB1;1, resulting increase size length. Higher repress promote 5 (ABI5) expression. elevates SHY2 expression, thereby impairing activity retarding growth. Notably, ABI5 can only absence ABI3. Such concentration-dependent therefore functions as regulatory sensor orchestrates coordinating its downstream regulation.

Язык: Английский

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The B‐box protein BBX13/COL15 suppresses photoperiodic flowering by attenuating the action of CONSTANS in Arabidopsis

Plant Cell & Environment, Год журнала: 2024, Номер 47(12), С. 5358 - 5371

Опубликована: Авг. 27, 2024

The optimal timing of transition from vegetative to floral reproductive phase is critical for plant productivity and agricultural yields. Light plays a decisive role in regulating this transition. B-box (BBX) family transcription factors regulates several light-mediated developmental processes plants, including flowering. Here, we identify previously uncharacterized group II BBX member, BBX13/COL15, as negative regulator flowering under long-day conditions. BBX13 primarily expressed the leaf vasculature, buds, flowers, showing similar spatial expression pattern major time regulators CO FT. bbx13 mutants flower early, while BBX13-overexpressors exhibit delayed long days. Genetic analyses showed that acts upstream FT negatively their expression. physically interacts with inhibits CO-mediated transcriptional activation In addition, directly binds CORE2 motif on promoter, where also binds. Chromatin immunoprecipitation data indicates reduces vivo binding promoter. Through luciferase assay, found Together, these findings suggest BBX13/COL15 represses Arabidopsis by attenuating

Язык: Английский

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BBX22 enhances the accumulation of antioxidants to inhibit DNA damage and promotes DNA repair under high UV‐B

Physiologia Plantarum, Год журнала: 2025, Номер 177(1)

Опубликована: Янв. 1, 2025

Abstract Under changing climatic conditions, plant exposure to high‐intensity UV‐B can be a potential threat health and all plant‐derived human requirements, including food. It's crucial understand how plants respond high radiation so that proper measures taken enhance tolerance towards stress. We found BBX22 , B‐box protein‐coding gene, is strongly induced within one hour of UV‐B. Our metabolomics data indicated promotes the accumulation antioxidants like ascorbic acid proline. These play vital role in shielding exposed from detrimental effects Reactive Oxygen Species (ROS), DNA damage. Additionally, damage repair by inducing expression genes UVR1 UVR3 . directly binds promoter regulate its expression. Furthermore, indirectly induces enhancing binding HY5 their promoters. Together, these results suggest multi‐pronged protection against Enhancing levels or orthologs different species potentially offer intense UV radiation.

Язык: Английский

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Exploring ABI5 regulation: Post‐translational control and cofactor interactions in ABA signaling

The Plant Journal, Год журнала: 2025, Номер 121(3)

Опубликована: Фев. 1, 2025

SUMMARY Abscisic acid insensitive 5 (ABI5) is a pivotal transcription factor in abscisic (ABA) signaling, playing an essential role plant growth and responses to abiotic stress. This key regulator subject multifaceted regulation, especially on post‐translational mechanisms. Recent research has shed light the regulation of ABI5, encompassing both modifications (PTMs) modulation its transcriptional activity. In this review, we provide comprehensive overview current knowledge surrounding along with influence various cofactors activity protein stability. The potential biological roles PTMs ABI5 context ABA signaling stress are also explored. As one most extensively studied proteins environmental responses, sophisticated precise understanding regulatory mechanisms that govern not only beneficial for application genetic engineering but helpful our exploration fundamental principles regulation.

Язык: Английский

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