bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 13, 2023
Abstract
BBXs
are
B-Box
zinc
finger
proteins
that
can
act
as
transcription
factors
and
regulators
of
protein
complexes.
Several
BBX
play
important
roles
in
plant
development.
Two
Arabidopsis
thaliana
microProteins
belonging
to
the
family,
named
miP1a
miP1b,
homotypically
interact
with
modulate
activity
other
proteins,
including
CONSTANS,
which
transcriptionally
activates
florigen,
FLOWERING
LOCUS
T
.
In
tomato,
closest
homologs
miP1b
Sl
BBX16
BBX17.
To
deepen
our
understanding
role
tomato
flowering,
we
constitutively
expressed
BBX16/17
examined
possible
interacting
partners.
Overexpression
two
caused
a
delay
flowering
transition;
however,
effect
was
weaker
than
observed
plants
overexpressing
native
miP1a/b.
overexpression
SlBBX17
prolonged
period;
this
accompanied
by
downregulation
inhibitors
Self
Pruning
(
SP
)
SP5G
BBX17
able
hetero-oligomerize
TCMP-2,
cystine-knot
peptide
involved
pattern
fruit
development
tomato.
Increasing
expression
both
also
alterations
development:
resulted
diminished
number
size
ripe
fruits
compared
WT
plants,
while
SlBBX16
delayed
production
up
breaker
stage.
These
effects
were
associated
changes
genes
regulating
gibberellin
content.
Summary
In
terrestrial
plants,
seeds
often
germinate
and
initiate
early
development
in
the
darkness
under
layers
of
soil.
Soil
overlay
promotes
ethylene
accumulation
to
maintain
hook
until
seedlings
reach
soil
surface.
During
this
phase
etiolated
growth
emergence,
perceive
changing
light
fluence.
Coordinated
interplay
signaling
pathways
ensures
optimal
timing
apical
opening
enhanced
emergence.
Here,
we
report
that
cooperatively
act
on
B‐box
protein
BBX32
regulate
enhance
emergence
.
Ethylene
induces
expression
dark.
CONSTITUTIVE
PHOTOMORPHOGENIC
1
degrades
dark
while
enhances
during
de‐etiolation.
bbx32
mutants
exhibit
accelerated
opening,
overexpressing
lines
show
delayed
opening.
Genetic
analyses
reveal
functions
concert
with
PHYTOCHROME‐INTERACTING
FACTOR
3
negatively
HOOKLESS
a
PIF3‐dependent
manner
delay
Under
cover,
35S:BBX32
reduced
seedling
respectively.
Our
study
illustrates
role
synchronizing
ethylene‐mediated
Journal of Experimental Botany,
Год журнала:
2024,
Номер
75(16), С. 5111 - 5129
Опубликована: Май 20, 2024
Plants
combat
dehydration
stress
through
different
strategies
including
root
architectural
changes.
Here
we
show
that
when
exposed
to
varying
levels
of
stress,
primary
growth
in
Arabidopsis
is
modulated
by
regulating
meristem
activity.
Abscisic
acid
(ABA)
concert
with
auxin
signalling
adjust
according
levels.
ABSCISIC
ACID
INSENSITIVE
3
(ABI3),
an
ABA-responsive
transcription
factor,
stands
at
the
intersection
ABA
and
fine-tunes
response
stress.
Under
low
or
induction
ABI3
expression
promotes
decreasing
SHY2,
a
negative
regulator
response.
This
further
enhances
transporter
gene
PIN1
cell
cycle
CYCB1;1,
resulting
increase
size
length.
Higher
repress
promote
5
(ABI5)
expression.
elevates
SHY2
expression,
thereby
impairing
activity
retarding
growth.
Notably,
ABI5
can
only
absence
ABI3.
Such
concentration-dependent
therefore
functions
as
regulatory
sensor
orchestrates
coordinating
its
downstream
regulation.
Plant Physiology and Biochemistry,
Год журнала:
2024,
Номер
213, С. 108873 - 108873
Опубликована: Июнь 21, 2024
BBXs
are
B-Box
zinc
finger
proteins
that
can
act
as
transcription
factors
and
regulators
of
protein
complexes.
Several
BBX
play
important
roles
in
plant
development.
Two
Arabidopsis
thaliana
microProteins
belonging
to
the
family,
named
miP1a
miP1b,
homotypically
interact
with
modulate
activity
other
proteins,
including
CONSTANS,
which
transcriptionally
activates
florigen,
FLOWERING
LOCUS
T.
plants
overexpressing
miP1b
showed
delayed
flowering.
In
tomato,
closest
homologs
SlBBX16
SlBBX17.
This
study
was
aimed
at
investigating
whether
constitutive
expression
SlBBX16/17
tomato
impacted
reproductive
The
heterologous
two
caused
a
delay
flowering
transition;
however,
effect
weaker
than
observed
when
native
miP1a/b
were
overexpressed.
overexpression
SlBBX17
prolonged
period;
this
accompanied
by
downregulation
inhibitors
Self
Pruning
(SP)
SP5G.
hetero-oligomerize
TCMP-2,
cystine-knot
peptide
involved
pattern
regulation
early
fruit
development
tomato.
increased
both
also
alterations
development:
we
case
decrease
number
size
ripe
fruits
compared
WT
plants,
while
for
SlBBX16,
production
up
breaker
stage.
These
effects
associated
changes
GA-responsive
genes.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(21), С. 11654 - 11654
Опубликована: Окт. 30, 2024
Double
B-box
(DBB)
proteins
are
plant-specific
transcription
factors
(TFs)
that
play
crucial
roles
in
plant
growth
and
stress
responses.
This
study
investigated
the
classification,
structure,
conserved
motifs,
chromosomal
locations,
cis-elements,
duplication
events,
expression
levels,
protein
interaction
network
of
DBB
TF
family
genes
common
wheat
(
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 19, 2024
Summary
The
optimal
timing
of
transition
from
vegetative
to
floral
reproductive
phase
is
critical
for
plant
productivity
and
agricultural
yields.
Light
plays
a
decisive
role
in
regulating
this
transition.
B-box
(BBX)
family
transcription
factors
regulates
several
light-mediated
developmental
processes
plants,
including
flowering.
Here,
we
identify
previously
uncharacterized
group
II
member
the
BBX
family,
BBX13/COL15,
as
negative
regulator
flowering
under
long-day
conditions.
BBX13
primarily
expressed
leaf
vasculature,
buds,
flowers.
Its
spatial
expression
pattern
similar
major
time
regulators
CO
FT.
bbx13
mutants
flower
early,
while
-overexpressors
exhibit
delayed
long
days.
Genetic
analyses
showed
that
acts
upstream
FT
pathway
negatively
their
expression.
We
found
physically
interacts
with
inhibits
CO-mediated
transcriptional
activation
.
In
addition,
directly
binds
CORE2
motif
on
promoter,
site
where
also
binds.
Furthermore,
our
ChIP
data
indicates
reduces
vivo
binding
promoter.
Through
luciferase
assay,
All
these
together
suggest
BBX13/COL15
represses
Arabidopsis
by
attenuating
activity.
statement
BBX13,
protein
B-Box
factors,
thaliana
genetically
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 13, 2023
Abstract
BBXs
are
B-Box
zinc
finger
proteins
that
can
act
as
transcription
factors
and
regulators
of
protein
complexes.
Several
BBX
play
important
roles
in
plant
development.
Two
Arabidopsis
thaliana
microProteins
belonging
to
the
family,
named
miP1a
miP1b,
homotypically
interact
with
modulate
activity
other
proteins,
including
CONSTANS,
which
transcriptionally
activates
florigen,
FLOWERING
LOCUS
T
.
In
tomato,
closest
homologs
miP1b
Sl
BBX16
BBX17.
To
deepen
our
understanding
role
tomato
flowering,
we
constitutively
expressed
BBX16/17
examined
possible
interacting
partners.
Overexpression
two
caused
a
delay
flowering
transition;
however,
effect
was
weaker
than
observed
plants
overexpressing
native
miP1a/b.
overexpression
SlBBX17
prolonged
period;
this
accompanied
by
downregulation
inhibitors
Self
Pruning
(
SP
)
SP5G
BBX17
able
hetero-oligomerize
TCMP-2,
cystine-knot
peptide
involved
pattern
fruit
development
tomato.
Increasing
expression
both
also
alterations
development:
resulted
diminished
number
size
ripe
fruits
compared
WT
plants,
while
SlBBX16
delayed
production
up
breaker
stage.
These
effects
were
associated
changes
genes
regulating
gibberellin
content.