Temperature and light reverse the fertility of rice P/TGMS line ostms19 via reactive oxygen species homeostasis

Plant Biotechnology Journal, Год журнала: 2024, Номер 22(7), С. 2020 - 2032

Опубликована: Фев. 29, 2024

Summary P/TGMS (Photo/thermo‐sensitive genic male sterile) lines are crucial resources for two‐line hybrid rice breeding. Previous studies revealed that slow development is a general mechanism sterility–fertility conversion of in Arabidopsis. However, the difference genes between and Arabidopsis suggests presence distinct rice. In this study, we isolated novel line, ostms19 , which shows sterility under high‐temperature conditions fertility low‐temperature conditions. OsTMS19 encodes pentatricopeptide repeat (PPR) protein essential pollen formation, point mutation GTA(Val) to GCA(Ala) leads phenotype. It highly expressed tapetum localized mitochondria. Under high temperature or long‐day photoperiod conditions, excessive ROS accumulation anthers during mitosis disrupts gene expression intine causing sterility. Conversely, low short‐day can be effectively scavenged anthers, resulting restoration. This indicates homeostasis critical conversion. relationship has also been observed other tested lines. Therefore, propose

Язык: Английский

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The OsMYC2–JA feedback loop regulates diurnal flower‐opening time via cell wall loosening in rice

The Plant Journal, Год журнала: 2024, Номер 119(6), С. 2585 - 2598

Опубликована: Июль 7, 2024

Diurnal flower-opening time (DFOT), the of spikelet opening during day, is an important trait for hybrid rice (Oryza sativa L.) seed production. Hybrids between indica and japonica varieties have strong heterosis, but parental lines usually different, nonoverlapping DFOTs. This reduces success production in crosses subspecies, thus hindering utilization inter-subspecies heterosis. However, little known about molecular mechanisms regulating DFOT rice. Here, we obtained with a 1.5 h earlier than wild type by overexpressing OsMYC2, gene encoding key transcription factor jasmonate (JA) signaling pathway. OsMYC2 activated JA directly regulates genes related to biosynthesis cell wall metabolism. Overexpressing led significantly increased contents decreased cellulose hemicellulose lodicule cells, as well softening walls. may facilitate swelling lodicules, resulting early diurnal flower-opening. These results suggest that OsMYC2-JA feedback loop via remodeling. findings shed light on understanding regulatory mechanism plants, which should promote development suitable breeding.

Язык: Английский

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Natural variation in CTF1 conferring cold tolerance at the flowering stage in rice

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Summary Improving cold tolerance at the flowering stage (CTF) in rice is crucial for minimising yield loss, making identification and application of cold‐tolerant genes QTLs imperative effective molecular breeding. The long lead time, dependence on treatment conditions, inherent complexity trait make studying genetic basis CTF challenging. To date, fine‐mapping or cloning specific to has not yet been achieved. In this study, single segment substitution lines (SSSLs) were constructed using HJX74 as recipient IR58025B, known good CTF, donor. This approach led two QTLs, qCTF3 qCTF6 , rice. promising breeding potential. Further, we identified causal gene CTF1 underlying through map‐based . which encodes a conserved putative protein, SNPs within its coding sequence that influence Additionally, variations promoter also contributes CTF. Thirteen variant sites four SSSLs are consistent with IR58025B. Moreover, analysed 307 accessions characterise haplotypes based 13 variation sites, identifying five distinct haplotypes. selection evolutionary analysis indicate haplotype newly generated mutation undergone japonica during domestication. study only provides novel favourable but highlights potential advancing

Язык: Английский

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tRNA selectivity during ribosome-associated quality control regulates the critical sterility-inducing temperature in two-line hybrid rice

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(6)

Опубликована: Фев. 6, 2025

The two-line hybrid rice system, a cutting-edge breeding technology, has greatly boosted global food security. In thermo-sensitive genic male sterile (TGMS) lines, the critical sterility-inducing temperature (CSIT; at which TGMS lines change from fertile to complete sterile) acts as key threshold. We recently uncovered that sterility 5 ( tms5 ), locus presenting in over 95% of leads overaccumulation 2′,3′-cyclic phosphate (cP)-ΔCCA-tRNAs and deficiency mature tRNAs, underlies molecular mechanism -mediated TGMS. However, there are few reports on regulatory controlling CSIT. Here, we identified suppressor , an amino acid substitution (T552I) Rqc2 (ribosome-associated quality control 2), increases CSIT through its C-terminal alanine threonine modification (CATylation) activity. This alters tRNA selectivity, leading recruitment different tRNAs A-site ribosome CATylation rate by OsRqc2 during ribosome-associated (RQC), process rescues stalled ribosomes degrades abnormal nascent chains translational elongation. Further, mutation restores levels tRNA-Ser/Ile increase lines. Our findings reveal origin overaccumulated cP-ΔCCA-tRNAs further deepening our understanding network governing containing tms5.

Язык: Английский

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核糖体介导的翻译调控与植物环境温度适应

Chinese Science Bulletin (Chinese Version), Год журнала: 2025, Номер 70(7), С. 807 - 815

Опубликована: Фев. 8, 2025

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Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Rice serves as a staple food for approximately half of the world's population, and enhanced yields from hybrid rice play crucial role in ensuring security augmenting incomes. However, annual purchase high cost seeds hinder widespread adoption. In this review, we discuss seed production strategies based on molecular mechanisms along with biotechnological techniques employed future prospects. Male-sterile lines are pivotal production, ongoing developments markedly advancing process. Initially, cytoplasmic male-sterile facilitated three-line production. Subsequent innovations, including environmentally responsive gene-based biotechnology-driven lines, enabled two-line Ongoing research is focusing implementing one-line method using apomixis, driving innovation Overall, advancements synthetic apomixis present promising avenues improving efficiency sustainability These highlight critical need continued concerted efforts to address global challenges.

Язык: Английский

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Molecular mechanisms underlying the negative effects of transient heatwaves on crop fertility

Plant Communications, Год журнала: 2024, Номер 5(9), С. 101009 - 101009

Опубликована: Июнь 24, 2024

Язык: Английский

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The E3 ligase OsHel2 impedes readthrough of stalled mRNAs to regulate male fertility in thermo-sensitive genic male sterile rice

Plant Communications, Год журнала: 2024, Номер 6(2), С. 101192 - 101192

Опубликована: Ноя. 13, 2024

Heterosis is extensively used in the 2-line hybrid breeding system. Photosensitive/thermosensitive genic male sterile (P/TGMS) lines are key components of rice, and TGMS containing tms5 have significantly advanced rice breeding. We cloned TMS5 gene found that a tRNA cyclic phosphatase can remove 2',3'-cyclic phosphate (cP) from cP-ΔCCA-tRNAs for efficient repair to ensure maintenance mature levels. mutation causes increased levels reduced tRNAs, leading sterility at restrictive temperatures. However, regulatory network tms5-mediated remains be clarified. Here, we demonstrate E3 ligase OsHel2 cooperates with regulate Consistently, both accumulation reduction tRNAs mutant largely recovered oshel2-1 mutant. A lesion results partial readthrough stalled sequences, thereby enabling evasion ribosome-associated protein quality control (RQC) surveillance. Our findings reveal mechanism by which impedes mRNA sequences fertility providing paradigm investigating how disorders RQC pathway impair cellular functions lead diseases or defects other organisms.

Язык: Английский

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The F box protein INHIBITOR FOR BROWN FURROWS 1 (IBF1) regulates flavonoid accumulation in rice hull by promoting degradation of chalcone synthase 1

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

SUMMARY Flavonoids are secondary metabolites of plants that play various roles in plants. The transcriptional level regulation flavonoid synthesis has been extensively studied, but research on the protein is still limited. In present study, a brown hull mutant, bh2 , was screened from an ethane methyl sulfonate (EMS)‐induced bank seeds indica cultivar RH2B. mutant exhibited phenotype and higher levels total flavonoids anthocyanins compared with wild‐type We identified gene INHIBITOR FOR BROWN FURROWS 1 ( IBF1 ) through MutMap analysis subsequently cloned it. encodes F‐box involved formation SCF (S‐phase kinase‐associated [SKP1], Cullin, F‐box) complex Oryza sativa SKP1‐like proteins OSK1/OSK20. Through yeast two‐hybrid, bimolecular fluorescence complementation, pull‐down assays, interaction chalcone synthase (CHS1) confirmed. This facilitated degradation CHS1 ubiquitin‐26S proteasome system. ibf1 chs1 double mutants normal color, restoring . Genetic suggested regulates color CHS1‐dependent manner. Collectively, our study suggests serves as crucial negative regulator controls biosynthesis by mediating degradation, thereby regulating color.

Язык: Английский

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OsNRAMP7 positively regulates heat tolerance at seedling and reproductive stages in rice

Plant Stress, Год журнала: 2025, Номер unknown, С. 100870 - 100870

Опубликована: Апрель 1, 2025

Язык: Английский

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