Reshaping epigenomic landscapes in facilitating the speciation of bread wheat DOI Creative Commons
Zhaoheng Zhang, Xuelei Lin, Jingjing Yue

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 27, 2024

Summary Polyploidization is a driving force of wheat evolution and speciation, yet its impact on epigenetic regulation gene expression remains unclear. Here, we constructed high-resolution landscape across leaves, spikes, roots hexaploidy tetraploid diploid relatives. Inter-species stable-expression genes exhibited conserved amino acid sequences under strong purifying selection, while dynamic-expression were linked to species-specific adaptation. During hexaploidization, dominant D-subgenome homoeolog was suppressed via reduced activating signals, converging with the A B subgenomes. Proximal chromatin regions near more stable, whereas distal regions, particularly enhancer-like elements mediated by H3K27ac H3K4me3, exhibit higher dynamism. Sequence variations in these enhancers lead differential regulation, influencing traits such as spike development. For instance, two haplotypes dCRE region TaDEP-B1 resulted significant differences spikelet numbers. We also observed coevolution transcription factors their binding sites, within expanded ERF family, which regulates morphology. This study highlights interplay between sequence variation modifications shaping transcriptional during offering valuable insights for genetic improvement.

Язык: Английский

Epigenetics in the modern era of crop improvements DOI Creative Commons
Yan Xue, Xiaofeng Cao, Xiangsong Chen

и другие.

Science China Life Sciences, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Abstract Epigenetic mechanisms are integral to plant growth, development, and adaptation environmental stimuli. Over the past two decades, our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body knowledge on both locus-specific genome-wide patterns. Studies initially grounded in model Arabidopsis have been broadened encompass diverse array crop species, revealing multifaceted roles epigenetics physiological agronomic traits. With recent technological advancements, epigenetic regulations at single-cell level large-scale population emerging as new focuses. This review offers an in-depth synthesis regulations, detailing catalytic machinery functions. It delves into intricate interplay among various elements their collective influence modulation Furthermore, it examines breakthroughs technologies for modifications integration strategies improvement. The underscores transformative potential bolstering performance, advocating development efficient tools fully exploit agricultural benefits insights.

Язык: Английский

Процитировано

1

Recent allopolyploidization and transcriptomic asymmetry in the mangrove shrub Acanthus tetraploideus DOI Creative Commons
Wuxia Guo, Achyut Kumar Banerjee, Hui Feng

и другие.

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Май 2, 2025

Mangrove species are vital to the ecosystems of tropical and subtropical coastlines worldwide. Despite underexplored role polyploidization in these species, deciphering its impact on gene expression is essential for understanding connection between diversification. Our initial investigation, integrating multiple nuclear loci with morphological cytological data, indicates that tetraploid Acanthus tetraploideus likely originated from allopolyploidization events involving diploid A. ilicifolius ebracteatus. Expanding insights, this study utilizes genome-wide evidence confirm divergence patterns among extant mangrove diploids investigate origin transcriptome asymmetry tetraploideus. Phylogenetic analysis molecular dating revealed a closer evolutionary relationship ebracteatus volubilis than ilicifolius, diverged approximately 6.92 Mya 9.59 Mya, respectively. Analysis individual whole transcriptomes homeologous sequences were preferentially clustered ebracteatus, rather volubilis, roughly 1:1 ratio. The high similarity nucleotide homologous polymorphisms two parental diploids, supports hypothesis recent allopolyploid Estimation homeolog general attenuation compared silico mix, 22.87% 67.66% genes exhibiting biased expression, Further investigation identified remarkable retention dominance tetraploid, suggesting genetic legacy substantially influences reconfiguration derived tetraploid. Meanwhile, observation numerous novel sets suggests shock (i.e., transcriptomic changes induced by interspecific hybridization) associated subsequent post-polyploid processes also significantly While no strong directly links specific adaptive traits, unbiased novelly suggest adaptations stable polyploidy. Unbiased involved fundamental cellular related chromosome dynamics cell cycle regulation may stabilize polyploid genomes, supporting species' establishment long-term success. These findings underscore stability adaptation. sheds light origins intricate transcriptional insights enhance our comprehension pivotal plays speciation adaptative evolution species.

Язык: Английский

Процитировано

0

Epigenetic perspectives on wheat speciation, adaptation, and development DOI
Xuemei Liu, Dongzhi Wang,

Zhaoheng Zhang

и другие.

Trends in Genetics, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

In vitro induction of tetraploids and their phenotypic and transcriptome analysis in Glehnia littoralis DOI Creative Commons
Xin Zhang, Ziyu Zheng, Jing Wang

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Май 22, 2024

Abstract Background Glehnia littoralis is a medicinal and edible plant species having commercial value has several hundred years of cultivation history. Polyploid breeding one the most important fastest ways to generate novel varieties. To obtain tetraploids G. in vitro, colchicine treatment was given seeds then were screened based on morphology, flow cytometry, root tip pressing assays. Furthermore, transcriptome analysis performed identity differentially expressed genes associated with phenotypic changes tetraploid . Results The results showed that 0.05% (w/v) for 48 h effective inducing (2n = 4x 44) superior leaf area, thickness, petiole diameter, SPAD (Chl SPAD), stomatal size, epidermal tissues palisade spongy thickness diploid ones, while density significantly lower. Transcriptome sequencing revealed, total 1336 (DEGs) between diploids. Chromosome doubling may lead DNA content change gene dosage effect, which directly affects quantitative traits, such as increased chlorophyll content, larger stomata thicker tissue leaves. Several up-regulated DEGs found related growth development CKI , PPDK hisD MDP1 KEGG pathway enrichment analyses enriched metabolic pathways. Conclusions This first report successful induction information presented this study facilitate programs molecular

Язык: Английский

Процитировано

3

Integrated multi-omics reveals contrasting epigenetic patterns in leaf and root morphogenesis in Aegilops speltoides DOI Creative Commons
Ling-Feng Miao, Zhe Chen,

Lifeng Gao

и другие.

The Crop Journal, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

1

Reshaping epigenomic landscapes in facilitating the speciation of bread wheat DOI Creative Commons
Zhaoheng Zhang, Xuelei Lin, Jingjing Yue

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 27, 2024

Summary Polyploidization is a driving force of wheat evolution and speciation, yet its impact on epigenetic regulation gene expression remains unclear. Here, we constructed high-resolution landscape across leaves, spikes, roots hexaploidy tetraploid diploid relatives. Inter-species stable-expression genes exhibited conserved amino acid sequences under strong purifying selection, while dynamic-expression were linked to species-specific adaptation. During hexaploidization, dominant D-subgenome homoeolog was suppressed via reduced activating signals, converging with the A B subgenomes. Proximal chromatin regions near more stable, whereas distal regions, particularly enhancer-like elements mediated by H3K27ac H3K4me3, exhibit higher dynamism. Sequence variations in these enhancers lead differential regulation, influencing traits such as spike development. For instance, two haplotypes dCRE region TaDEP-B1 resulted significant differences spikelet numbers. We also observed coevolution transcription factors their binding sites, within expanded ERF family, which regulates morphology. This study highlights interplay between sequence variation modifications shaping transcriptional during offering valuable insights for genetic improvement.

Язык: Английский

Процитировано

0