A Year at the Forefront of Streptophyte Algal Evolution DOI Creative Commons
Alexander M. C. Bowles

Biology Open, Год журнала: 2024, Номер 13(9)

Опубликована: Сен. 15, 2024

ABSTRACT Land plants originated from an algal ancestor ∼500 million years ago in one of the most important evolutionary events for life on Earth. Extant streptophyte algae, their closest living relatives, have subsequently received much attention to better understand this major transition. Streptophyte algae occupy many different environments, diverse genomes and display contrasting morphologies (e.g. unicellular, filamentous, three-dimensional). This has historically made inferring these challenging. A Year at Forefront Review focusses research published between July 2023 June 2024 intends provide a short overview recent discoveries, innovations, resources, hypotheses regarding evolution. work provided mechanistic insights into ancient that prefigured origin land raises new questions future algae.

Язык: Английский

Comparative transcriptomics elucidates the cellular responses of an aeroterrestrial zygnematophyte to UV radiation DOI Creative Commons
Anna Busch, Jennifer V. Gerbracht, Kevin M. Davies

и другие.

Journal of Experimental Botany, Год журнала: 2024, Номер 75(11), С. 3624 - 3642

Опубликована: Март 23, 2024

The zygnematophytes are the closest relatives of land plants and comprise several lineages that adapted to a life on land. Species genus Serritaenia form colorful, mucilaginous capsules, which surround cells block harmful solar radiation, one major terrestrial stressors. In eukaryotic algae, this 'sunscreen mucilage' represents unique photoprotective strategy, whose induction chemical background unknown. We generated de novo transcriptome testaceovaginata studied its gene regulation under moderate UV radiation (UVR) triggers sunscreen mucilage experimental conditions. UVR induced repair DNA photosynthetic apparatus as well synthesis aromatic specialized metabolites. Specifically, we observed pronounced expressional changes in production amino acids, phenylpropanoid biosynthesis genes, potential cross-membrane transporters phenolics, extracellular, oxidative enzymes. Interestingly, most up-regulated enzyme was secreted class III peroxidase, embryophyte homologs involved apoplastic lignin formation. Overall, our findings reveal conserved, plant-like perception system (UVR8 downstream factors) zygnematophyte algae point polyphenolic origin pigment Serritaenia, might be extracellular oxidative, resembling plant lignins.

Язык: Английский

Процитировано

6
An ice‐binding protein from the glacier ice alga Ancylonema nordenskioeldii DOI Open Access
James A. Raymond, Lenka Procházková, Daniel Remias

и другие.

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

As plants began to colonize the land c. 470–450 million years ago, they had overcome many abiotic stresses not experienced by their marine ancestors (Rensing, 2018). One such stress was freezing and thawing, which can damage plant cell walls. Bacteria, established presence on well before arrival of plants, greatly aided transition horizontal transfer key genes (Yue et al., 2012; Ma 2020). Bacteria are likely donors that mitigate freeze–thaw injury, as proteins with ice-binding activity have been found in several bacteria (Raymond 2007, 2008; Vance Each those studies contains a 200-aa domain called DUF3494 has beta solenoid structure one side binds ice crystals (Vance 2019). At very low concentrations, these drastically prevent recrystallization occurs during is thought Similar identified hundreds species bacteria, although all them examined for activity. Horizontally acquired this type appear be source tolerance number algae fungi live icy habitats & Kim, 2012). Zygnematophyceae, being closest known relatives (Cheng 2019), interest because remarkable ability find solutions encountered early (Kunz 2024). In vein, Bowles al. (2024) recently obtained metagenome inhabiting Morteratsch Glacier Switzerland investigate adaptation streptophyte alga Ancylonema nordenskioeldii life ice. Among survival mechanisms investigated, authors looked encoding (IBPs). They candidates protein kinase superfamily, ATP-binding cassette family heat shock family, none were confirmed Apparently, did see our earlier paper we an IBP A. (AnIBP) from (Procházková Here, summarize main findings paper, showed AnIBP could attributed family. The alaskanum dominate Glacier, relative abundances vary year Other chlorophyte relatively small numbers. Initially, sample activity, is, cause distortions form pitting faceting growing crystal (fig. 3 Procházková destroyed treatment, strongly suggesting active substance protein. Furthermore, retained after three cycles ice-affinity purification (Fig. 1). We search ice-active A problem finding IBPs habitat metagenomes include fungi. few DUF3494-type genes, most or remained puzzle until submitted sequence data Closterium (Zygnematophyceae) (Sekimoto 2023) unambiguously it belonged nordenskioeldii. predicted (AnIBP, GenBank WHL30856) consisted N-terminal signal peptide, domain, sandwich C-terminal root cap domain. curious feature mostly cells vascular where involved sensing interacting environment (Kumpf Nowack, 2015). based 100% identical metagenome. gene length 515 aa molecular mass 54.5 kDa. No homologs alaskanum, other Zygnematophyceae GenBank. appears use glycerol instead cryoprotectant structures domains confidently AlphaFold3, shown local distance difference test (pLDDT) values. show updated using AlphaFold4, somewhat lower pLDDT values but virtually same 2). populated ordered array hydrophilic threonine serine residues whose spacings close a-axis repeat 3D seen more clearly Supporting Information Video S1. 2D electrophoretic gel ice-affinity-purified Fig. 1 prominent spot expected weight pI 5 phylogenetic tree shows AnIBP's relations its closely related archaea, S1). algal snow (Chloromonas brevispina), bootstrap nearest neighbors low, leaving origin unclear. bacterial seems (see Discussion). To examine expression AnIBP, complete sequences two common housekeeping glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ribosomal L13a (RPL13a), assembled RNAseq PacBio (Notes Five 100-nt each used query (The conditions under stated, daytime glacier conditions.) These expressed over 12 times than GAPDH eight RPL13a (Table So far, 1000 identified, microorganisms. enormously size, some having others, unknown function. Many nonfreezing habitats, may bind substrates even individual water molecules way mitigating desiccation. sides numerous TXT motifs (where X any amino acid), (Graether Sykes, 2004). Some allowed survive desiccation soil This case no introns does match databases. Its matching lichen Alberta, Canada (MCJ1363104), bacterium Antarctic moss (ALG05169), both only 50% acid identities AnIBP. also introns, bacterium. On hand, dozens algae, characteristic Zygnematophyceae. especially grasses (e.g. Kuiper 2001). solenoid, significantly differs ones proteins. nordenskioeldii, belongs group streptophytes, It thus grass-type developed later evolution best available streptophytes study supported Charles University Research Centre program grant no. UNCE/24/SCI/006 LP, Austrian Science Fund (FWF) P-34073 DR personal funds JAR. None declared. LN designed original carried out collections. JAR analyzed wrote paper. at accession WHL30856. S1 Phylogenetic matches. Notes Sequences analysis. Table Relative expressions (2024). movie rotating 2. Please note: Wiley responsible content functionality supplied authors. Any queries (other missing material) should directed New Phytologist Central Office. publisher supporting information content) corresponding author article. Foundation remains neutral regard jurisdictional claims maps institutional affiliations.

Язык: Английский

Процитировано

0
Functional genomic perspectives on plant terrestrialization DOI Creative Commons
Cäcilia F. Kunz, Elisa S. Goldbecker, Jan de Vries

и другие.

Trends in Genetics, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Genome evolution: Zygnematophyceae on ice DOI Open Access
Cäcilia F. Kunz, Elisa S. Goldbecker, Tatyana Darienko

и другие.

New Phytologist, Год журнала: 2024, Номер 244(4), С. 1125 - 1127

Опубликована: Июль 12, 2024

This article is a Commentary on Bowles et al . (2024), 244 : 1629–1643.

Язык: Английский

Процитировано

1
A Year at the Forefront of Streptophyte Algal Evolution DOI Creative Commons
Alexander M. C. Bowles

Biology Open, Год журнала: 2024, Номер 13(9)

Опубликована: Сен. 15, 2024

ABSTRACT Land plants originated from an algal ancestor ∼500 million years ago in one of the most important evolutionary events for life on Earth. Extant streptophyte algae, their closest living relatives, have subsequently received much attention to better understand this major transition. Streptophyte algae occupy many different environments, diverse genomes and display contrasting morphologies (e.g. unicellular, filamentous, three-dimensional). This has historically made inferring these challenging. A Year at Forefront Review focusses research published between July 2023 June 2024 intends provide a short overview recent discoveries, innovations, resources, hypotheses regarding evolution. work provided mechanistic insights into ancient that prefigured origin land raises new questions future algae.

Язык: Английский

Процитировано

0