Chitin soil amendment triggers systemic plant disease resistance through enhanced pattern-triggered immunity DOI Creative Commons

Moffat Makechemu,

Y Goto, Helen Zbinden

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 10, 2024

Summary Chitin triggers localised and systemic plant immune responses, making it a promising treatment for sustainable disease resistance. However, the precise molecular mechanisms underlying chitin-induced effects in plants remain unknown. In this study, we investigated of soil amendment with crab chitin flakes (hereafter chitin) on pattern-triggered immunity (PTI) resistance various species. We found that potentiates PTI against bacterial pathogen Pseudomonas syringae pv. tomato DC3000 lettuce, tomato, Arabidopsis as well fungal Blumeria graminis causing powdery mildew wheat. Using micrografting Arabidopsis, demonstrated effect is dependent active perception roots. also showed induced (ISR) pattern-recognition receptors (PRRs)/co-receptors, but not acquired (SAR), are involved triggered by amendment. This correlated transcriptional up-regulation key components distal leaves upon Notably, chitin-triggered was independent microbes present or flakes. Together, these findings contribute to better understanding immunity, from roots potentiation leaves, ultimately priming mount enhanced defense responses attacks. Our study provides valuable insights into resulting highlights its potential use crop protection strategies.

Язык: Английский

BIK1 and ATL31/6 are essential for fucoidan hydrolysate-triggered stomatal closure in Arabidopsis DOI

Kexin Chen,

Guofu Yan,

Jie Tang

и другие.

Biochemical and Biophysical Research Communications, Год журнала: 2025, Номер 772, С. 152041 - 152041

Опубликована: Май 17, 2025

Язык: Английский

Процитировано

0
The Medicago truncatula LYR4 intracellular domain serves as a scaffold in immunity signaling independent of its phosphorylation activity DOI Open Access
Bine Simonsen, Henriette Rübsam, Marie Vogel Kolte

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 25, 2024

Abstract Plants perceive and respond to chitin derived from fungal cell walls through lysine motif (LysM) receptor kinases. In the model legume Medicago truncatula , CERK1 LYR4 represent LysM pair important for chitin-triggered immunity signaling. Here, we show that both active kinase pseudokinase contribute signaling, leading production of reactive oxygen species (ROS). We determine crystal structure core intracellular domain with a bound nucleotide analog in site. Biochemical characterization shows binds ATP has autophosphorylation as well transphosphorylation activity towards CERK1. However, planta experiments demonstrate phosphorylation ability is not necessary function ROS production, but presence its indispensable. Together, serves signaling scaffold independent catalytic activity.

Язык: Английский

Процитировано

0
Chitin soil amendment triggers systemic plant disease resistance through enhanced pattern-triggered immunity DOI Creative Commons

Moffat Makechemu,

Y Goto, Helen Zbinden

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 10, 2024

Summary Chitin triggers localised and systemic plant immune responses, making it a promising treatment for sustainable disease resistance. However, the precise molecular mechanisms underlying chitin-induced effects in plants remain unknown. In this study, we investigated of soil amendment with crab chitin flakes (hereafter chitin) on pattern-triggered immunity (PTI) resistance various species. We found that potentiates PTI against bacterial pathogen Pseudomonas syringae pv. tomato DC3000 lettuce, tomato, Arabidopsis as well fungal Blumeria graminis causing powdery mildew wheat. Using micrografting Arabidopsis, demonstrated effect is dependent active perception roots. also showed induced (ISR) pattern-recognition receptors (PRRs)/co-receptors, but not acquired (SAR), are involved triggered by amendment. This correlated transcriptional up-regulation key components distal leaves upon Notably, chitin-triggered was independent microbes present or flakes. Together, these findings contribute to better understanding immunity, from roots potentiation leaves, ultimately priming mount enhanced defense responses attacks. Our study provides valuable insights into resulting highlights its potential use crop protection strategies.

Язык: Английский

Процитировано

0