Malignant Transformation of Proliferative Verrucous Leukoplakia: A Description of the Clinical Oral Characteristics of These Squamous Cell Carcinomas

Cancers, Год журнала: 2025, Номер 17(7), С. 1199 - 1199

Опубликована: Апрель 1, 2025

Background/Objectives: Proliferative verrucous leukoplakia (PVL) is an oral disorder with the greatest degree of malignant transformation. However, it relatively rare. This study compared clinical characteristics patients squamous cell carcinoma (OSCC) who had and not been previously diagnosed PVL. Methods: case control classified as early (T1 T2) or advanced (T3 T4) OSCC according to T size Tumor, Node, Metastasis (TNM) classification, including age, gender, location, type carcinoma. The analysis involved 140 patients. Group 1: 50 PVL (OSCC-PVL) 2: 90 without (OSCC-noPVL). Results: OSCC-PVL were younger than those OSCC-noPVL, but this did reach statistical significance. Regarding patient much more frequently female (70%), while OSCC-noPVL was prevalent in men (65.5%) (p < 0.01). There also significant differences locations between two groups: gingiva most tongue OSCC-noPVL. Erythroleukoplastic forms significantly common (30% vs. 7.7%), ulcerated frequent (63.3% 42%). Finally, stages our OSCC-PVL. Conclusions: We found that preceded by women, less aggressive forms,

Язык: Английский

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Genome-Wide DNA Methylation Confirms Oral Squamous Cell Carcinomas in Proliferative Verrucous Leukoplakia as a Distinct Oral Cancer Subtype: A Case–Control Study

Cancers, Год журнала: 2025, Номер 17(2), С. 245 - 245

Опубликована: Янв. 13, 2025

Background/Objectives: Oral cancers in patients with proliferative verrucous leukoplakia (PVL-OSCC) exhibit different clinical and prognostic outcomes from those seen conventional oral squamous cell carcinomas (cOSSCs). The aim of the present study is to compare genome-wide DNA methylation signatures fresh frozen tissues between PVL cOSCC using Illumina Infinium MethylationEPIC BeadChip. Methods: This case–control was carried out at Stomatology Maxillofacial Surgery Department General University Hospital Valencia. For epigenomic study, unsupervised exploratory bioinformatic analyses were performed principal component heatmap analysis. Supervised differential conducted a rank-based regression model penalized logistic identify potential biomarkers. Results: global profiles did not allow us differentiate distinct cancer groups. However, two supervised confirmed existence carcinoma phenotypes. We identified 21 differentially methylated CpGs corresponding 14 genes. Among them, three had been previously assigned any known gene, remaining associated genes unrelated cancer. AGL, WRB, ARL15 as Conclusions: emphasizes significant role epigenetic dysregulation OSCC, particularly cases preceded by PVL. have provided data on that could be involved molecular carcinogenesis PVL-OSCC.

Язык: Английский

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Specific Oral Microbial Differences in Proteobacteria and Bacteroidetes Are Associated with Distinct Sites When Moving from Healthy Mucosa to Oral Dysplasia—A Microbiome and Gene Profiling Study and Focused Review

Microorganisms, Год журнала: 2023, Номер 11(9), С. 2250 - 2250

Опубликована: Сен. 7, 2023

Oral potentially malignant disorders (OPMDs) are a group of conditions that carry risk oral squamous cell carcinoma (OSCC) development. Recent studies indicate periodontal disease-associated pathogenic bacteria may play role in the transition from healthy mucosa to dysplasia and OSCC. Yet, microbial signatures associated with have not been established. To characterize at these different sites, we performed 16S sequencing analysis both swab formalin-fixed, paraffin-embedded tissue (FFPE) samples. We collected swabs (from patients), histologically normal adjacent dysplasia, low-grade dysplasia. Additionally, FFPE samples OSCC, plus low grade high-grade were also collected. The data demonstrate significant differences alpha beta diversities sites mucosa, as well increased dissimilarities within sites. found Proteobacteria phyla abundance increased, concurrent progressive decrease Firmicutes abundance, altered levels Enterococcus cecorum, Fusobacterium periodonticum, Prevotella melaninogenica, canifelinum when moving diseased Moreover, sample indicates microbiome be areas normal, including Furthermore, trends specific changes preceded those tissues, signifying early detection opportunities for clinical diagnosis. In addition, evaluated gene expression profile OSCC cells (HSC-3) infected either P. gingivalis, T. denticola, F. nucelatum, or S. sanguinis three periodontopathogens enrich genetic processes related cancer progression, skin keratinization/cornification, while commensal enriched RNA processing adhesion. Finally, reviewed literature bacteria, such Streptococci genus, simultaneous increase mainly Bacteroidetes genus. These findings suggest features can serve novel biomarkers disease progression.

Язык: Английский

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Proliferative verrucous and homogeneous Leukoplakias exhibit differential methylation patterns

Oral Diseases, Год журнала: 2024, Номер unknown

Опубликована: Июнь 9, 2024

Abstract Objective Proliferative verrucous leukoplakia (PVL) is considered a clinically distinct entity from other oral leucoplakias (OLs) due to its clinical presentation and evolution. However, molecular differences between them remain unclear. We aimed determine whether there are methylation PVL forms of OLs. Materials Methods Oral biopsies 12 patients with PVL, eight homogeneous leucoplakia (HL), 10 healthy individuals were obtained for genome‐wide DNA analysis via the Infinium EPIC Platform. Results A total 1815 differentially methylated CpGs found HL, prominent state hypermethylation in HL patients. covered 813 genes roles, including cell adhesion, extracellular matrix organization, synaptic signaling. 43% these had been previously described cancer associated prognosis. developed multinomial logistic regression model able differentiate control samples. The cross‐validated estimate 73% included cancer‐related pathological conditions donors, ADNP , BRCA2 CDK13 GNB1 NIN NUMB PIK3C2B PTK2 SHISA4 THSD7B WWP1 ZNF292 . It also covering ( MEN1 TNRC6B ) ACOXL ADH1B CAMTA1 CBFA2T3 CPXM2 LRFN2 SORCS2, SPN ). Conclusions present differential patterns that could be linked their behavior. Our findings show potential markers suggest novel diagnostic biomarkers.

Язык: Английский

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A Bibliometric and Visualised Analysis of Proliferative Verrucous Leucoplakia From 2003 to 2023

International Dental Journal, Год журнала: 2024, Номер unknown

Опубликована: Июль 1, 2024

Proliferative verrucous leucoplakia (PVL) is a rare but slow-growing, aggressive lesion associated with the highest malignant transformation rate in oral potentially disorders (OPMDs). With increasing attention paid to PVL, it urgent for us analyse and summarise publications globally using comprehensive bibliometric studies help researchers propose possible future research directions guide them further conduct relevant domain. The purpose of study was evaluate global academic productivity, impact, collaboration disorder PVL utilising bibliometrics based on annual number publications, countries regions, institution, authors, journals, citations co-occurrences author keywords over last 20 years. This searched pertaining proliferative Web Science Core Collection, spanning from 2003 2023. Utilizing VOSviewer, R software, Bibliometric online analysis platform, CiteSpace Microsoft Excel, we conducted visualised PVL. quantity pertinent this domain displays fluctuating overall upward trend. In aggregate, there are 148 articles 61 reviews, encompassing contributions 44 countries, 45 institutions, involving 831 authors. Among these USA, Spain, UK emerged as predominant contributing nations. Predominantly, found their publication venue "Pathology Research Practice." Notably, most influence Warnakulasuriya S. top 3 include "Proliferative Verrucous Leucoplakia," "Squamous-Cell Carcinoma," "Oral "Potentially Malignant Disorders." investigation, statistical network visualisation were reveal progress, trends, trending topics via thorough analysis. We that current comprise mainly case reports, significant need explore molecular mechanisms, specific diagnostic criteria, effective management options Our work should serve key reference directional

Язык: Английский

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Prognostic nomogram for proliferative verrucous leukoplakia

Journal of Dental Sciences, Год журнала: 2024, Номер 20(1), С. 238 - 247

Опубликована: Авг. 13, 2024

Proliferative verrucous leukoplakia (PVL) is a special type of characterized by high rate malignant transformation into oral squamous cell carcinoma (OSCC). This study aimed to analyze the canceration risk and prognostic factors PVL establish effective diagnostic predictive models. A total 467 patients were enrolled, including 170 cases PVL. The independent analyzed univariable multivariable logistic regression. Nomogram models constructed predict prognosis power was evaluated Hosmer-Lemeshow test, receiver operating characteristic (ROC) curve, calibration curve decision analysis. Multivariable regression analyses identified that included sex, lesion sites, clinical presentation, non-smoker epithelial dysplasia (OED). local irritants OED. Diagnosis nomogram constructed. areas under ROC 0.945 0.893, respectively. plots showed strong agreement between prediction observation. Decision analysis indicated provided significant benefits for patients. Our established validated diagnosis models, which accurate PVL, providing individualized decisions work.

Язык: Английский

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Proliferative verrucous leukoplakia: Case study of 24 years and outcome of treatment with CO₂ laser

Clinical Advances in Periodontics, Год журнала: 2024, Номер unknown

Опубликована: Окт. 24, 2024

Abstract Background Proliferative verrucous leukoplakia (PVL) is a rare and refractory form of oral leukoplakia. The etiology PVL remains unknown. lesion characterized by high rate malignant transformation. There no definitive treatment for PVL. Methods This case study presents patient diagnosed in 2000 with localized hyperkeratinized/verrucous involving the facial gingivae teeth #6–#8. Over next 24 years, was biopsied five times. Further, two attempts to ablate CO 2 laser (10.6 µm wavelength) were performed. Both ablation treatments unsuccessful as recurred progressed involve more areas gingival tissue. To date, biopsy has not revealed transformation into or squamous cell carcinoma (SCCA). Results demonstrates that at using had short‐ long‐term benefit. eventually lost all maxillary due root caries inability maintain adequate hygiene. currently involves entire edentulous ridge, extending from 2nd molar site opposite corresponding site. During years encompassed this report, experienced Conclusion results provided Given potential sinister outcome, patients require frequent clinical evaluation early detection SCCA. Key points diagnosis represents multifocal mucosal unknown origin. Currently, there reliable successful may undergo carcinoma, thereby necessitating examination sinister‐appearing sites. Plain language summary disorder affects gum tissue around teeth. pre‐cancerous which cause yields consistently result. upper right cuspid, lateral, central incisor times irradicate All slowly last taken February 2024 did reveal any developing cancer. covered develop However, afforded measurable cancer tissues, should receive exams periodic biopsies

Язык: Английский

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Proliferative verrucous leukoplakia, an enigma to the pathologists: Report of two cases

SAGE Open Medical Case Reports, Год журнала: 2024, Номер 12

Опубликована: Янв. 1, 2024

The oral cavity presents with an array of white lesions ranging from physiological alteration to extensive malignant entities. Among them, proliferative verrucous leukoplakia is a rare highly aggressive multifocal form that poses high risk for transformation. Etiopathogenesis and its diagnostic criteria have remained speculative since inception. diagnosis this challenging it requires updated knowledge expertise identify condition. All the cases are resistant treatment chances recurrence. In present case report, we aim report document two leukoplakia, which were diagnosed in dental college. can serve as guide young surgeons spot refer them tertiary care hospitals treatment.

Язык: Английский

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Progression of Oral Leukoplakia to Cancer: Importance on Collection of Leukoplakia and Cancer Tissues from Disparate or Same Patients

Cancer Screening and Prevention, Год журнала: 2024, Номер 3(2), С. 118 - 124

Опубликована: Июнь 25, 2024

Oral squamous cell carcinoma (OSCC) is a predominant type of head and neck cancer in the Indian subcontinent, mostly observed among tobacco and/or alcohol users. leukoplakia (OLK) does not seriously affect patients, so it often ignored treatment. Some studies have reported genomic alterations expression deregulation that drive OLK towards OSCC, conducted two types based on sample collection from (a) disparate or (b) same patients. Demographic, tobacco/alcohol habits biological factors may vary significantly if OSCC samples are collected but they remain consistent both tissue patient. Earlier, targeted candidate gene-based large-scale omics-based identified somatic mutations TP53, CDKN2A, PTEN, as well broad arm-level copy number epigenetically dysregulated genes tumor tissues Recent early CASP8 alterations, APOBEC mutagenesis, immune infiltration (decreased CD8+ T abundance, enrichment pro-inflammatory cells) driver events for oral progression single-cell transcriptomic-driven also immune-transcriptomic features putative driving molecular development progression. Here, we reviewed differences gene deregulations patient settings. We highlighted challenges opportunity genomics transcriptome their emerging role diagnosis

Язык: Английский

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DNA methylation analysis from oral brushing reveals a field cancerization effect in proliferative verrucous leukoplakia

Pathologica, Год журнала: 2024, Номер 116(6), С. 368 - 378

Опубликована: Дек. 1, 2024

The aim of the present study was to analyze methylation status in patients who presented with an Oral Squamous Cell Carcinoma (OSCC) concomitantly multifocal Proliferative Verrucous Leukoplakia (PVL)(PVL-OSCC).

Язык: Английский

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