Annual Review of Phytopathology,
Год журнала:
2019,
Номер
57(1), С. 211 - 230
Опубликована: Июнь 11, 2019
Plant
viruses
were
first
implemented
as
heterologous
gene
expression
vectors
more
than
three
decades
ago.
Since
then,
the
methodology
for
their
use
has
varied,
but
we
propose
it
was
merging
of
technologies
with
virology
tools,
which
occurred
in
defined
steps
discussed
here,
that
driven
viral
vector
applications
to
date.
The
advent
molecular
biology
and
reverse
genetics,
enabled
cloning
manipulation
genomes
express
genes
interest
(vectors
1.0).
second
stems
from
discovery
RNA
silencing
development
high-throughput
sequencing
allowed
convenient
widespread
virus-induced
2.0).
Here,
briefly
review
events
led
these
applications,
this
treatise
mainly
concentrates
on
emerging
versatility
gene-editing
emergence
virus-delivered
genetic
queries
functional
genomics
3.0).
Horticulturae,
Год журнала:
2024,
Номер
10(1), С. 57 - 57
Опубликована: Янв. 6, 2024
The
development
and
improvement
of
molecular
biology
methods
have
led
to
the
creation
new
technologies
that
make
it
possible
modify
plant
genomes
by
transferring
integrating
into
genomes’
heterologous
genes
from
various
expression
systems
(genetic
engineering),
as
well
inducing
knockouts
one
or
more
target
interest
(genomic
editing).
genome-editing
is
a
milestone
in
modern
breeding
certainly
relies
on
knowledge
developed
for
transgenesis.
This
review
will
discuss
issues
related
advantages
disadvantages
both
improving
economically
valuable
traits
important
crops.
Abstract
Global
agriculture
and
food
security
are
encountering
unprecedented
challenges
from
both
the
ever-growing
population
rapidly
changing
climate
conditions.
CRISPR/Cas-mediated
genome
editing
technology
has
revolutionized
plant
functional
genetic
research
precision
crop
breeding
with
robustness,
high
target
specificity
programmability.
Furthermore,
numerous
emerging
biotechnologies
based
on
CRISPR/Cas
platform
provide
opportunity
to
create
new
germplasms
durable
resistance
against
disease
or
insect
pests,
herbicide
tolerance,
other
stress-tolerant
improvements,
reshaping
protection
increase
agricultural
resilience
sustainability.
In
this
review,
we
briefly
describe
toolbox,
including
base
editing,
prime
compact
manipulation,
transcriptional
regulation
epigenetic
then
overview
most
important
applications
of
improvement,
highlighting
protection-based
stress
engineering.
addition,
enumerate
global
regulations
genome-edited
crops.
Finally,
discuss
some
bottlenecks
facing
cutting-edge
infinite
possibilities
for
future.
Plants,
Год журнала:
2025,
Номер
14(3), С. 477 - 477
Опубликована: Фев. 6, 2025
CRISPR/Cas9-mediated
homology-directed
repair
(HDR)
is
a
powerful
tool
for
precise
genome
editing
in
plants,
but
its
efficiency
remains
low,
particularly
targeted
amino
acid
substitutions
or
gene
knock-ins.
Successful
HDR
requires
the
simultaneous
presence
of
Cas9,
guide
RNA,
and
template
(RT)
same
cell
nucleus.
Among
these,
timely
availability
RT
at
double-strand
break
(DSB)
site
critical
bottleneck.
To
address
this,
we
developed
sequential
transformation
strategy
incorporating
deconstructed
wheat
dwarf
virus
(dWDV)-based
autonomously
replicating
delivery
system,
effectively
simplifying
process
into
two-component
system.
Using
this
approach,
successfully
achieved
OsEPSPS
rice
with
10
percent
efficiency,
generating
three
lines
(TIPS1,
TIPS2,
TIPS3)
(T172I
P177S)
native
EPSPS
protein.
The
modifications
were
confirmed
through
Sanger
sequencing
restriction
digestion
assays,
edited
showed
no
yield
penalties
compared
to
wild-type
plants.
This
study
demonstrates
utility
viral
replicons
delivering
gene-editing
tools
modification,
offering
promising
approach
efficient
crop
improvement
programs.
Advances in Agriculture,
Год журнала:
2025,
Номер
2025(1)
Опубликована: Янв. 1, 2025
Clustered
regularly
interspaced
short
palindromic
repeats/CRISPR‐associated
protein
9
(CRISPR/Cas9)
stands
as
a
major
technique
in
genetic
engineering,
offering
innumerable
applications
for
enhancing
agricultural
and
horticultural
crops.
It
contributes
significantly
to
improving
quality
traits,
managing
biotic
abiotic
stresses,
extending
shelf
life.
Several
genes,
such
Cry
genes
or
protease
inhibitors
trypsin
inhibitors,
have
been
utilized
confer
resistance
against
insect
pests
diseases.
This
finds
extensive
application
medicine,
agricultural,
CRISPR/Cas9
has
successfully
implemented
tomato,
petunia,
grapes,
apple,
citrus,
yielding
promising
results.
For
instance,
employed
with
the
Agrobacterium
gene
transfer
method
provide
powdery
mildew
disease.
review
presents
comprehensive
overview
of
achievements
Overall,
this
highlights
potential
CRISPR
technology
plant
improvement,
limitations
challenges
including
off‐target
mutations,
size
system,
delivery,
bio‐safety
regulations
improve
variety
crop
yield,
quality,
stress
tolerance,
disease,
herbicide
resistance.
can
also
be
used
speed
up
improvement
compared
conventional
breeding.
Annual Review of Phytopathology,
Год журнала:
2019,
Номер
57(1), С. 211 - 230
Опубликована: Июнь 11, 2019
Plant
viruses
were
first
implemented
as
heterologous
gene
expression
vectors
more
than
three
decades
ago.
Since
then,
the
methodology
for
their
use
has
varied,
but
we
propose
it
was
merging
of
technologies
with
virology
tools,
which
occurred
in
defined
steps
discussed
here,
that
driven
viral
vector
applications
to
date.
The
advent
molecular
biology
and
reverse
genetics,
enabled
cloning
manipulation
genomes
express
genes
interest
(vectors
1.0).
second
stems
from
discovery
RNA
silencing
development
high-throughput
sequencing
allowed
convenient
widespread
virus-induced
2.0).
Here,
briefly
review
events
led
these
applications,
this
treatise
mainly
concentrates
on
emerging
versatility
gene-editing
emergence
virus-delivered
genetic
queries
functional
genomics
3.0).
