A MADS-box protein GhAGL8 promotes early flowering and increases yield without compromising fiber quality in cotton

Industrial Crops and Products, Год журнала: 2025, Номер 225, С. 120545 - 120545

Опубликована: Янв. 24, 2025

Язык: Английский

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Decoding cellular transcriptional regulatory networks governing wheat inflorescence development

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 12, 2025

In wheat, inflorescence architecture critically determines yield potential, yet its structural complexity and asynchronous development have hindered cellular-resolution studies of spikelet floret formation. Here, we integrate spatial transcriptomics, high-sensitivity multiplexed error-robust fluorescence in situ hybridization (MERFISH), snRNA-seq across six developmental stages to generate a spatiotemporal atlas the wheat inflorescence. We identified 20 cell types, spatially resolved into three categories: 1) proliferating cells within spikelet, marked by active division; 2) supporting along central axis, including pith, cortex, vasculature; 3) developmental located both inside at base spikelets. The multi-omics approach enabled identification rare type ovary. Trajectory inference revealed that spikelets florets originate from two temporally distinct sub-clusters proliferating cells (R7), each defined high expression regulators. These findings challenge conventional model sequential meristem transitions (inflorescence-spikelet-floret) in wheat. Integration time-series snATAC-seq delineated cellular transcriptional regulatory networks (cTRNs) governing formation, mediated auxin cytokinin signaling, driven MADS-box transcription factors. Cell identity was maintained type-specific accessible chromatin regions (csACRs), which are enriched for SNPs associated with spike-related traits. For instance, csACRs WFZP DUO1 promoters affect TaNAC30 binding, regulating supernumerary phenotypes. Our work provides mechanistic framework identifies cTRN nodes as potential targets optimizing yield-related architecture.

Язык: Английский

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QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheat

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 10, 2025

Spike traits play a pivotal role in determining wheat yield. In this study, two recombinant inbred line (RIL) populations were employed to identify quantitative trait loci (QTLs) for spike length (SL), spikelet number per (SNS), and basal sterile (BSSN). A total of 30 QTLs identified, including 7 major-effect that exhibited stability across multiple environments. Among these, novel discovered: QBssn.A-3 A.3 QBssn.AV-5B.2. Furthermore, it was found the combination these with other major effect had no impact on thousand-kernel weight (TKW). For A.3, Kompetitive Allele-Specific PCR (KASP) marker developed validated based its physical location. Additionally, candidate genes within intervals QBssn.AV-5B.2 predicted, primarily associated spike. This study provides foundation future map-baesd cloning related traits, breeding high-yield varieties.

Язык: Английский

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Overexpression of the WAPO-A1 gene increases the number of spikelets per spike in bread wheat

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Авг. 20, 2022

Two homoeologous QTLs for number of spikelets per spike (SPS) were mapped on chromosomes 7AL and 7BL using two wheat MAGIC populations. Sets lines contrasting the QTL developed which allowed validation fine mapping identification a previously described candidate gene, WHEAT ORTHOLOG OF APO1 (WAPO1). Using transgenic overexpression in both low high SPS line, we provide functional role this gene determining also hexaploid wheat. We show that expression levels positively correlate with multiple founder under field conditions as well grown greenhouse. This work highlights potential use WAPO1 further yield increases. The impact depends other genetic environmental factors, hence, will require finely balanced level to avoid development detrimental pleiotropic phenotypes.

Язык: Английский

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A co-located QTL for seven spike architecture-related traits shows promising breeding use potential in common wheat (Triticum aestivum L.)

Theoretical and Applied Genetics, Год журнала: 2024, Номер 137(1)

Опубликована: Янв. 1, 2024

Язык: Английский

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