Genetic Improvement of Camelina sativa (L.) Crantz: Opportunities and Challenges

Plants, Год журнала: 2023, Номер 12(3), С. 570 - 570

Опубликована: Янв. 27, 2023

In recent years, a renewed interest in novel crops has been developing due to the environmental issues associated with sustainability of agricultural practices. particular, cover crop, Camelina sativa (L.) Crantz, belonging Brassicaceae family, is attracting scientific community's for several desirable features. It related model species Arabidopsis thaliana, and its oil extracted from seeds can be used either food feed, or industrial uses such as biofuel production. From an agronomic point view, it grow marginal lands little no inputs, practically resistant most important pathogens Brassicaceae. Although cultivated past, particularly northern Europe Italy, last century, was abandoned. For this reason, breeding work conducted improve plant, also because low genetic variability present hexaploid species. review, we summarize main works on focused improvement three objectives: yield, seed content quality, reduction glucosinolates seed, which are anti-nutritional substances camelina. We report latest advances utilising classical plant breeding, transgenic approaches, CRISPR-Cas9 genome-editing.

Язык: Английский

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Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways

Plant Communications, Год журнала: 2023, Номер 4(4), С. 100565 - 100565

Опубликована: Фев. 23, 2023

Glucosinolates (GSLs), found mainly in species of the Brassicaceae family, are one most well-studied classes secondary metabolites. Produced by action myrosinase on GSLs, GSL-derived hydrolysis products (GHPs) primarily defend against biotic stress planta. They also significantly affect quality crop products, with a subset GHPs contributing unique food flavors and multiple therapeutic benefits or causing disagreeable odors health risks. Here, we explore potential these bioactive functions, which could be exploited for future sustainable agriculture. We first summarize our accumulated understanding GSL diversity distribution across representative species. then systematically discuss evaluate unutilized genes involved biosynthesis, transport, as candidate engineering targets. Benefiting from available information GHP options multifunctional ideotypes to meet demand diversification production. An integrated roadmap is subsequently proposed guide ideotype development, maximization beneficial effects minimization detrimental combined associated various end uses. Based several use-case examples, advantages limitations biotechnological approaches that may contribute effective deployment provide novel insights optimization engineering.

Язык: Английский

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Multi‐omic analyses unveil contrasting composition and spatial distribution of specialized metabolites in seeds of Camelina sativa and other Brassicaceae

The Plant Journal, Год журнала: 2025, Номер 121(3)

Опубликована: Фев. 1, 2025

SUMMARY Seeds of Brassicaceae produce a large diversity beneficial and antinutritional specialized metabolites (SMs) that influence their quality provide resistance to stresses. While SM distribution has been described in leaves root tissues, limited information is available about spatiotemporal accumulation seeds. Camelina sativa (camelina) an oilseed cultivated for human animal nutrition industrial uses. we previously explored plasticity, no expression related proteins genes camelina In this study, used multi‐omic approach, integrating untargeted metabolomics, proteomics, transcriptomics investigate the synthesis, modification, degradation SMs accumulated seed tissues (seed coat, endosperm, embryo) at six developmental two germination stages. Metabolomic results showed distinct patterns pathways, highlighting significant contrasts composition spatial defense‐related glucosinolate (GSL) compounds among camelina, Arabidopsis thaliana , Brassica napus three closely species. Notably, thanks metabolomic proteomic/transcriptomic techniques variation GSL distributions was primarily driven by differences structure (metabolomics data) transport (transcriptomic proteomic mechanisms. Long‐chain C8–C11 methylsulfinylalkyl GSLs were predominantly coat while mid‐ short‐chain C3–C7 embryo. Characterizing dynamics provides valuable insights can guide development crops with optimized toxic metabolites, improving nutritional profiles.

Язык: Английский

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Specialized metabolite modifications in Brassicaceae seeds and plants: diversity, functions and related enzymes

Natural Product Reports, Год журнала: 2024, Номер 41(5), С. 834 - 859

Опубликована: Янв. 1, 2024

Diversity, functions and related enzymes of Specialized Metabolite (SM) modifications from plants seeds Brassicaceae are summarized in this review. A particular focus is made on SM plasticity modification seeds.

Язык: Английский

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Comprehensive overview of glucosinolates in crucifers: occurrence, roles, metabolism, and transport mechanisms—a review

Phytochemistry Reviews, Год журнала: 2024, Номер unknown

Опубликована: Сен. 26, 2024

Язык: Английский

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Biofortification of health-promoting glucosinolates in cruciferous sprouts along the whole agro-food chain

Trends in Food Science & Technology, Год журнала: 2023, Номер 140, С. 104164 - 104164

Опубликована: Сен. 2, 2023

Язык: Английский

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Born of frustration: the emergence of Camelina sativa as a platform for lipid biotechnology.

PLANT PHYSIOLOGY, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Abstract The emerging crop Camelina sativa (L.) Crantz (camelina) is a Brassicaceae oilseed with rapidly growing reputation for the deployment of advanced lipid biotechnology and metabolic engineering. recognised by agronomists its traits including yield, oil/protein content, drought tolerance, limited input requirements, plasticity resilience. Its utility as platform engineering was then quickly recognised, biotechnologists have benefited from short life cycle facile genetic transformation, producing numerous transgenic interventions to modify seed content generate novel products. desire work plant that both model has driven expansion research resources camelina, increased availability genome other “-omics” data sets. Collectively these established camelina an ideal study regulation metabolism improvement. Furthermore, unique characteristics enables design-build-test-learn be transitioned controlled environment field. Complex synthesize accumulate high levels fatty acids modified oils in seeds, can deployed, tested undergo rounds iteration agronomically relevant environments. Engineered are now increasingly being developed used sustainably supply, improved nutrition, feed, biofuels fossil fuel replacements high-value chemical In this review, we provide summary acid synthesis oil assembly highlighting how discovery supports advance towards predictive manipulation produce desirable bio-based Further examples innovation improvement provided, describing technologies (e.g., modification (GM), gene editing (GE), RNAi, alongside GM GE stacking) applied new products denude undesirable traits. Focusing on production long chain polyunsaturated omega-3 describe transition commercial prototype. prospects structured triacylglycerol specified stereospecific positions also discussed, future outlook agronomic uptake biotechnology.

Язык: Английский

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Genetic transformation of the oilseed crop camelina using immature zygotic embryos

Plant Methods, Год журнала: 2025, Номер 21(1)

Опубликована: Март 13, 2025

Abstract Background Camelina is an oilseed crop with particularly useful fatty acid and amino profiles of its seeds, high resilience to abiotic biotic stresses, a short life cycle. Previous genetic engineering approaches in camelina have largely relied on the floral dip method which is, however, associated genotype-dependent efficiency incompatibility methods direct biomolecule delivery. Results Here, we established novel transgenesis for camelina, taking advantage regenerative capacity immature embryos. Various culture conditions treatments were experimentally validated, included duration explant pre-cultivation, wounding explants time application, Agrobacterium strain density inoculum, acetosyringone concentration, explant- co-cultivation, as well application concentration selective agent hygromycin. We provide convergent evidence stable transgenicity transgene inheritance by (1) selection resistance hygromycin, (2) PCR, (3) detection product GFP, (4) DNA gel blot analysis involving primary transgenic plants segregating progeny. Primary transgenics examined detail featured one three T-DNA integration loci, seven copies being integrated total per plant. The proved efficient across all tested accessions including two current cultivars, whereby transformation efficiencies, determined PCR-positive related agro-inoculated explants, between 13 17% obtained. Conclusion With this method, viable platform functional validation genes-of-interest biotechnological improvements plant performance quality features camelina.

Язык: Английский

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Synthetic biology and metabolic engineering strategies in identifying and producing plant natural products; with emphasis on the CRISPR/Cas systems

Industrial Crops and Products, Год журнала: 2025, Номер 230, С. 121060 - 121060

Опубликована: Апрель 23, 2025

Язык: Английский

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Plant Regeneration via Adventitious Shoot Formation from Immature Zygotic Embryo Explants of Camelina

Plants, Год журнала: 2024, Номер 13(4), С. 465 - 465

Опубликована: Фев. 6, 2024

Camelina is an oil seed crop that enjoying increasing interest because it has a particularly valuable fatty acid profile, modest regarding its water and nutrient requirements, comparatively resilient to abiotic biotic stress factors. The regeneration of plants from cells accessible genetic manipulation essential prerequisite for the generation genetically engineered plants, be by transgenesis or genome editing. Here, immature embryos were used on assumption their incomplete differentiation was associated with totipotency. In culture, regenerative structures appeared adventitiously at embryos’ hypocotyls. For this, application auxin- cytokinin-type growth regulators essential. formation most efficient when indole-3-acetic added induction medium 1 mg/L, zygotic walking stick stage used, hypocotyls stimulated pricking wound response. Histological examinations revealed adventitious shoots initiated locally activated cell division proliferation in epidermis outer cortex hypocotyl. While established principle using experimental line Cam139, method proved similarly applicable current cultivar Ligena, hence constitutes vital basis future engineering approaches.

Язык: Английский

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