Cucumber Green Mottle Mosaic Virus Coat Protein Hijacks Mitochondrial ATPδ to Promote Viral Infection DOI Creative Commons
Xue Yang,

Xing‐Lin Jiang,

Han Fu

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(11)

Опубликована: Ноя. 1, 2024

ABSTRACT The production and scavenging of reactive oxygen species (ROS) are critical for plants to adapt biotic abiotic stresses. In this study, we investigated the interaction between coat protein (CP) cucumber green mottle mosaic virus (CGMMV) ATP synthase subunit δ (ATPδ) in mitochondria. Silencing ATPδ by tobacco rattle virus‐based virus‐induced gene silencing impeded CGMMV accumulation Nicotiana benthamiana leaves. Both overexpression transgenic transient expression promoted infection. Nitro blue tetrazolium (NBT) 3,3′‐diaminobenzidine (DAB) staining revealed that inhibited O 2 − but not H production. treatment CGMMV‐infected leaves with ROS inhibitor diphenylene iodonium (DPI) induced a burst Reverse transcription‐quantitative PCR superoxide dismutase (SOD) activity assays showed ATPδ, infection, CP specifically NbFeSOD3/4 SOD activity, We speculate interacts hijacks it, thereby enhancing quenching upregulating NbFeSOD and, turn, activity.

Язык: Английский

Single-cell transcriptomes reveal spatiotemporal heat stress response in maize roots DOI Creative Commons
Ting Wang, Fanhua Wang,

Shuhan Deng

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 2, 2025

Plant roots perceive heat stress (HS) and adapt their architecture accordingly, which in turn influence the yield crops. Investigating heterogeneity cell type-specific response to HS is essential for improving crop resilience. Here, we generate single-cell transcriptional landscape of maize (Zea mays) HS. We characterize 15 clusters corresponding 9 major types identify cortex as main root type responsive with most differentially expressed genes its trajectory being preferentially affected upon find that size strongly correlated tolerance experimentally validated by using inbred lines genetic mutation analysis one candidate gene maize, providing potential indicator targets improvement. Moreover, interspecies comparison reveals conserved core markers plants, are validated. These results provide a universal atlas unraveling programs specify maintain identity at level. authors reveal relationship between tolerance.

Язык: Английский

Процитировано

4
Plant secondary metabolites against biotic stresses for sustainable crop protection DOI
Tanzim Jahan, Md. Nurul Huda, Kaixuan Zhang

и другие.

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108520 - 108520

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

3
Transcriptional landscape of cotton roots in response to salt stress at single-cell resolution DOI Creative Commons
Pengtao Li, Qiankun Liu, Yangyang Wei

и другие.

Plant Communications, Год журнала: 2023, Номер 5(2), С. 100740 - 100740

Опубликована: Окт. 29, 2023

Increasing soil salinization has led to severe losses of plant yield and quality. Thus, it is urgent investigate the molecular mechanism salt stress response. In this study, we took systematically analyzed cotton root response by single-cell transcriptomics technology; 56,281 high-quality cells were totally obtained from 5-days-old lateral tips Gossypium arboreum under natural growth different salt-treatment conditions. Ten cell types with an array novel marker genes synthetically identified confirmed in situ RNA hybridization, some specific-type pesudotime analysis also pointed out their potential differentiation trajectory. The prominent changes numbers responding observed on outer epidermal inner endodermic cells, which significantly enriched stress, amide biosynthetic process, glutathione metabolism, glycolysis/gluconeogenesis. Other functional aggregations concentrated plant-type primary wall biogenesis, defense response, phenylpropanoid biosynthesis metabolic pathways analyzing abundant differentially expressed (DEGs) multiple comparisons. Some candidate DEGs related transcription factors hormones identified, function Ga03G2153, annotated auxin-responsive GH3.6, was using virus-induced gene silencing (VIGS). GaGH3.6-silenced plants presented stress-susceptive phenotype, suffered more serious oxidative damages detecting physiological biochemical indexes, indicating that GaGH3.6 might participate tolerance through regulating oxidation-reduction process. For first time, a transcriptional atlas roots characterized at resolution, explored cellular heterogeneityand trajectory, providing valuable insights into underlying plants.

Язык: Английский

Процитировано

24
Spatiotemporal transcriptomic atlas of rhizome formation in Oryza longistaminata DOI Creative Commons
Xiaoping Lian, Liyuan Zhong,

Yixuan Bai

и другие.

Plant Biotechnology Journal, Год журнала: 2024, Номер 22(6), С. 1652 - 1668

Опубликована: Фев. 12, 2024

Summary Rhizomes are modified stems that grow underground and produce new individuals genetically identical to the mother plant. Recently, a breakthrough has been made in efforts convert annual grains into perennial ones by utilizing wild rhizomatous species as donors, yet developmental biology of this organ is rarely studied. Oryza longistaminata , rice featuring strong rhizomes, provides valuable model for exploration rhizome development. Here, we first assembled double‐haplotype genome O. which displays 48‐fold improvement contiguity compared previously published assembly. Furthermore, spatiotemporal transcriptomics was performed obtain expression profiles different tissues rhizomes tillers. Two spatially reciprocal cell clusters, vascular bundle 2 cluster parenchyma cluster, were determined be primary distinctions between We also captured meristem initiation cells sunken area located at base internodes, starting point initiation. Trajectory analysis further indicated regenerated through de novo generation. Collectively, these analyses revealed transcriptional transition underlying initiation, providing resource future crop breeding.

Язык: Английский

Процитировано

7
Development and application of transcriptomics technologies in plant science DOI Creative Commons
H.W. Wang, Yueting Xu, Zhizhong Zhang

и другие.

Crop Design, Год журнала: 2024, Номер 3(2), С. 100057 - 100057

Опубликована: Фев. 24, 2024

Over the past decade, bulk RNA sequencing (RNA-seq) has become an indispensable tool in molecular biology, and have made novel development, with two innovative methodologies being developed, single-cell (scRNA-seq) technology spatial transcriptome (ST) technology. The scRNA-seq allows researchers to analyze gene expression individual cells, providing more detailed information relative technologies. Meanwhile, ST overcomes limitations of terms loss information, enabling scientists better understand distribution within tissues. These advancements transcriptomics technologies revolutionize field genomics been widely used disease diagnosis medicine. However, they are less utilized plant research. This review describes advantage three technologies, presents their applications sciences.

Язык: Английский

Процитировано

7
From molecule to cell: the expanding frontiers of plant immunity DOI
Lei Li, J. Liu, Jian‐Min Zhou

и другие.

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2024, Номер 51(7), С. 680 - 690

Опубликована: Фев. 26, 2024

Язык: Английский

Процитировано

5
Hydrogen Peroxide Signaling in the Maintenance of Plant Root Apical Meristem Activity DOI Creative Commons
Hui Liu,

Yangwei Mu,

Yuxin Xuan

и другие.

Antioxidants, Год журнала: 2024, Номер 13(5), С. 554 - 554

Опубликована: Апрель 30, 2024

Hydrogen peroxide (H2O2) is a prevalent reactive oxygen species (ROS) found in cells and takes central role plant development stress adaptation. The root apical meristem (RAM) has evolved strong plasticity to adapt complex changing environmental conditions. Recent advances have made great progress explaining the mechanism of key factors, such as auxin, WUSCHEL-RELATED HOMEOBOX 5 (WOX5), PLETHORA (PLT), SHORTROOT (SHR), SCARECROW (SCR), regulation RAM activity maintenance. H2O2 functions an emerging signaling molecule control quiescent center (QC) specification stem cell niche (SCN) activity. Auxin signal for maintenance, which largely depends on formation auxin regional gradients. regulates gradients by modulation intercellular transport. also modulates expression WOX5, PLTs, SHR, SCR maintain present review dedicated summarizing factors discussing transduction maintenance significant

Язык: Английский

Процитировано

5
Between Host and Invaders: The Subcellular Cell Wall Dynamics at the Plant–Pathogen Interface DOI
Luís Pinto,

Luis Soler-López,

Antonio Serrano

и другие.

Annual Review of Plant Biology, Год журнала: 2025, Номер 76(1), С. 255 - 284

Опубликована: Май 20, 2025

Plant–pathogen interactions have profound ecological implications and are crucial for food security. Usually studied at the two extreme scales of plant organ symptomatology host–microbe molecules, they a cell–cell event mainly occurring subcellular level apoplast. Here, cell walls both organisms suffer an intense alteration as consequence active degradation by opponent self-protection mechanisms to survive continue growing. The wall modifications their role in defense danger signals activators signaling cascades been few decades, plane. Still, much remains unknown about this process, including cellular minority decorations, proteins, mechanical cues. Comparatively, microbial changes planta virtually unexplored. By investigating interface between biochemically, structurally, mechanically, we aim highlight dynamic interplay these areas its significance host–invader interaction.

Язык: Английский

Процитировано

0
Application of single-cell multi-omics approaches in horticulture research DOI Creative Commons
Jun Zhang,

Mayra Ahmad,

Hongbo Gao

и другие.

Molecular Horticulture, Год журнала: 2023, Номер 3(1)

Опубликована: Сен. 26, 2023

Abstract Cell heterogeneity shapes the morphology and function of various tissues organs in multicellular organisms. Elucidation differences among cells mechanism intercellular regulation is essential for an in-depth understanding developmental process. In recent years, rapid development high-throughput single-cell transcriptome sequencing technologies has influenced study plant biology. Additionally, accuracy sensitivity tools used to epigenome metabolome have significantly increased, thus enabling multi-omics analysis at resolution. Here, we summarize currently available approaches their applications research, review based studies fruit, vegetable, ornamental crops, discuss potential such future horticulture research. Graphical

Язык: Английский

Процитировано

9
单细胞测序在植物研究中的应用 DOI Open Access

Yanyan An,

Minghui Zhang,

Hexin Tan

и другие.

Chinese Science Bulletin (Chinese Version), Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

同一个基因在不同的细胞中表达存在一定的差异,常规的转录组测序方法(RNA-Seq)在组织或器官水平上进行分析,容易忽略细胞间的差异,但近几年迅速发展的高通量单细胞转录组测序技术在单细胞水平上分析,可以补充常规测序所忽略的异质性。目前,植物生物学领域已经全面开始单细胞转录组学,多组学研究成为趋势。本文综述了单细胞测序发展历程以及分离单细胞的方法并总结了各种方法的优缺点,介绍了近年来单细胞转录组测序技术在揭示植物细胞发育和分化过程、生物合成途径、应对生物与非生物胁迫的响应及在分析不同物种间细胞类型分化等方面取得的显著进展。此外,多组学联合应用不仅有助于我们更深入地理解植物生物学,也为植物育种等领域提供了新的研究方向。可以为将来更多在物种中广泛开展单细胞水平的研究提供参考。

Процитировано

3