Molecular Plant Pathology,
Год журнала:
2024,
Номер
25(11)
Опубликована: Ноя. 1, 2024
ABSTRACT
The
production
and
scavenging
of
reactive
oxygen
species
(ROS)
are
critical
for
plants
to
adapt
biotic
abiotic
stresses.
In
this
study,
we
investigated
the
interaction
between
coat
protein
(CP)
cucumber
green
mottle
mosaic
virus
(CGMMV)
ATP
synthase
subunit
δ
(ATPδ)
in
mitochondria.
Silencing
ATPδ
by
tobacco
rattle
virus‐based
virus‐induced
gene
silencing
impeded
CGMMV
accumulation
Nicotiana
benthamiana
leaves.
Both
overexpression
transgenic
transient
expression
promoted
infection.
Nitro
blue
tetrazolium
(NBT)
3,3′‐diaminobenzidine
(DAB)
staining
revealed
that
inhibited
O
2
−
but
not
H
production.
treatment
CGMMV‐infected
leaves
with
ROS
inhibitor
diphenylene
iodonium
(DPI)
induced
a
burst
Reverse
transcription‐quantitative
PCR
superoxide
dismutase
(SOD)
activity
assays
showed
ATPδ,
infection,
CP
specifically
NbFeSOD3/4
SOD
activity,
We
speculate
interacts
hijacks
it,
thereby
enhancing
quenching
upregulating
NbFeSOD
and,
turn,
activity.
Horticulture Research,
Год журнала:
2025,
Номер
12(4)
Опубликована: Янв. 3, 2025
Abstract
The
garlic
bulb
comprises
several
cloves,
the
swelling
growth
of
which
is
significantly
hindered
by
accumulation
viruses.
Herein,
we
describe
a
single-cell
transcriptomic
atlas
cloves
with
virus
accumulation,
comprised
19
681
high-quality
cells
representing
11
distinct
cell
clusters.
Cells
two
clusters,
clusters
7
(C7)
and
(C11),
were
inferred
to
be
from
meristem.
Cell
trajectory
analysis
suggested
differentiation
clove
start
meristem
cells,
along
pseudo-time
paths.
Investigation
into
cell-specific
activity
invasive
viruses
demonstrated
that
genes
showed
relatively
low-expression
in
There
2060
co-expressed
genes,
many
an
association
defense
response.
Five
glutathione
synthase/reductase
displayed
up-regulated
expression,
related
metabolites
level
alteration
virus-invasive
clove,
implying
role
viral
immunity
garlic.
Our
study
offers
valuable
insights
organogenesis
interaction
between
at
resolution.
Background:
The
passion
fruit
(Passiflora
edulis
Sims)
is
a
diploid
plant
(2n
=
2x
18)
and
perennial
scrambling
vine
in
Southern
China.
However,
the
occurrence
spread
of
stem
rot
severely
impact
its
yield
quality.
Methods:
In
this
study,
we
re-sequenced
BC1F1
population
consisting
158
individuals
using
whole-genome
resequencing.
We
constructed
high-density
genetic
linkage
map
identified
quantitative
trait
locus
(QTL),
analyzed
candidate
genes
associated
with
resistance
fruit.
Results:
Based
on
reference
genome
(MER),
was
1,180,406
single
nucleotide
polymorphisms
(SNPs).
contains
nine
groups,
covering
total
distance
1559.03
cM,
an
average
311.81
cM.
between
4206
bins
0.404
gap
length
10.565
collinearity
correlation
coefficient
0.9994.
Fusarium
solani
used
to
infect
population,
showed
continuous
distribution.
A
QTL,
qPSR5,
mapped
113,377,860
bp–114,811,870
bp
genomic
region
chromosome
5.
performed
RNA
sequencing
(RNA-seq)
real-time
polymerase
chain
reaction
(RT-qPCR)
analyze
expression
levels
predicted
ZX.05G0020740
ZX.05G0020810
as
ideal
for
Conclusions:
findings
study
not
only
lay
foundation
cloning
qPSR5
responsible
but
also
provide
resources
improvement
Cell Reports,
Год журнала:
2025,
Номер
44(2), С. 115240 - 115240
Опубликована: Фев. 1, 2025
Despite
the
broad
use
of
single-cell/nucleus
RNA
sequencing
in
plant
research,
accurate
cluster
annotation
less-studied
species
remains
a
major
challenge
due
to
lack
validated
marker
genes.
Here,
we
generated
single-cell
atlas
soil-grown
wheat
roots
and
annotated
identities
by
transferring
annotations
from
publicly
available
datasets
wheat,
rice,
maize,
Arabidopsis.
The
predictions
our
orthology-based
approach
were
next
using
untargeted
spatial
transcriptomics.
These
results
allowed
us
predict
evolutionarily
conserved
tissue-specific
markers
generate
cell
type-specific
gene
regulatory
networks
for
root
tissues
other
used
analysis.
In
summary,
transcriptomics
resource
apical
meristems,
including
numerous
known
uncharacterized
genes
developmental
regulators.
data
analyses
will
facilitate
future
type
non-model
species.
