Cucumber Green Mottle Mosaic Virus Coat Protein Hijacks Mitochondrial ATPδ to Promote Viral Infection DOI Creative Commons
Xue Yang,

Xing‐Lin Jiang,

Han Fu

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(11)

Опубликована: Ноя. 1, 2024

ABSTRACT The production and scavenging of reactive oxygen species (ROS) are critical for plants to adapt biotic abiotic stresses. In this study, we investigated the interaction between coat protein (CP) cucumber green mottle mosaic virus (CGMMV) ATP synthase subunit δ (ATPδ) in mitochondria. Silencing ATPδ by tobacco rattle virus‐based virus‐induced gene silencing impeded CGMMV accumulation Nicotiana benthamiana leaves. Both overexpression transgenic transient expression promoted infection. Nitro blue tetrazolium (NBT) 3,3′‐diaminobenzidine (DAB) staining revealed that inhibited O 2 − but not H production. treatment CGMMV‐infected leaves with ROS inhibitor diphenylene iodonium (DPI) induced a burst Reverse transcription‐quantitative PCR superoxide dismutase (SOD) activity assays showed ATPδ, infection, CP specifically NbFeSOD3/4 SOD activity, We speculate interacts hijacks it, thereby enhancing quenching upregulating NbFeSOD and, turn, activity.

Язык: Английский

A single-cell transcriptomic atlas reveals the cell differentiation trajectory and the response to virus invasion in swelling clove of garlic DOI Creative Commons
Song Gao, Fu Li, Zheng Zeng

и другие.

Horticulture Research, Год журнала: 2025, Номер 12(4)

Опубликована: Янв. 3, 2025

Abstract The garlic bulb comprises several cloves, the swelling growth of which is significantly hindered by accumulation viruses. Herein, we describe a single-cell transcriptomic atlas cloves with virus accumulation, comprised 19 681 high-quality cells representing 11 distinct cell clusters. Cells two clusters, clusters 7 (C7) and (C11), were inferred to be from meristem. Cell trajectory analysis suggested differentiation clove start meristem cells, along pseudo-time paths. Investigation into cell-specific activity invasive viruses demonstrated that genes showed relatively low-expression in There 2060 co-expressed genes, many an association defense response. Five glutathione synthase/reductase displayed up-regulated expression, related metabolites level alteration virus-invasive clove, implying role viral immunity garlic. Our study offers valuable insights organogenesis interaction between at resolution.

Язык: Английский

Процитировано

0

An overview of single-cell high-throughput technology in plants DOI
Lucas Auroux, Lim Chee Liew, Mathew G. Lewsey

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 1 - 34

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Single-cell technology for disease-resistant plants DOI

Lingaraj Dip,

Akbar Hossain, Srikrushna Behera

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 111 - 132

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Construction of a High-Density Genetic Linkage Map and QTL Mapping for Stem Rot Resistance in Passion Fruit (Passiflora edulis Sims) DOI Open Access

Yanyan Wu,

Weihua Huang,

Jieyun Liu

и другие.

Genes, Год журнала: 2025, Номер 16(1), С. 96 - 96

Опубликована: Янв. 17, 2025

Background: The passion fruit (Passiflora edulis Sims) is a diploid plant (2n = 2x 18) and perennial scrambling vine in Southern China. However, the occurrence spread of stem rot severely impact its yield quality. Methods: In this study, we re-sequenced BC1F1 population consisting 158 individuals using whole-genome resequencing. We constructed high-density genetic linkage map identified quantitative trait locus (QTL), analyzed candidate genes associated with resistance fruit. Results: Based on reference genome (MER), was 1,180,406 single nucleotide polymorphisms (SNPs). contains nine groups, covering total distance 1559.03 cM, an average 311.81 cM. between 4206 bins 0.404 gap length 10.565 collinearity correlation coefficient 0.9994. Fusarium solani used to infect population, showed continuous distribution. A QTL, qPSR5, mapped 113,377,860 bp–114,811,870 bp genomic region chromosome 5. performed RNA sequencing (RNA-seq) real-time polymerase chain reaction (RT-qPCR) analyze expression levels predicted ZX.05G0020740 ZX.05G0020810 as ideal for Conclusions: findings study not only lay foundation cloning qPSR5 responsible but also provide resources improvement

Язык: Английский

Процитировано

0

A single-cell and spatial wheat root atlas with cross-species annotations delineates conserved tissue-specific marker genes and regulators DOI Creative Commons
Yuji Ke, Vincent Pujol, Jasper Staut

и другие.

Cell Reports, Год журнала: 2025, Номер 44(2), С. 115240 - 115240

Опубликована: Фев. 1, 2025

Despite the broad use of single-cell/nucleus RNA sequencing in plant research, accurate cluster annotation less-studied species remains a major challenge due to lack validated marker genes. Here, we generated single-cell atlas soil-grown wheat roots and annotated identities by transferring annotations from publicly available datasets wheat, rice, maize, Arabidopsis. The predictions our orthology-based approach were next using untargeted spatial transcriptomics. These results allowed us predict evolutionarily conserved tissue-specific markers generate cell type-specific gene regulatory networks for root tissues other used analysis. In summary, transcriptomics resource apical meristems, including numerous known uncharacterized genes developmental regulators. data analyses will facilitate future type non-model species.

Язык: Английский

Процитировано

0

Decoding maize meristems maintenance and differentiation: integrating single-cell and spatial omics DOI
Bin Li, Wenhao Liu, Jie Xu

и другие.

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Opportunities and challenges in the application of single-cell transcriptomics in plant tissue research DOI
Man Luo, Yunpeng Cao,

Jiayi Hong

и другие.

Physiology and Molecular Biology of Plants, Год журнала: 2025, Номер 31(2), С. 199 - 209

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Single-nucleus transcriptome analysis reveals the cellular and molecular responses of tobacco roots to Ralstonia solanacearum invasion DOI Creative Commons
Meng Jia,

Xuanquan Zhu,

D. Y. Chen

и другие.

Industrial Crops and Products, Год журнала: 2025, Номер 226, С. 120752 - 120752

Опубликована: Фев. 27, 2025

Язык: Английский

Процитировано

0

Cellular heterogeneity and immune responses to smut pathogen in sugarcane DOI Creative Commons
Shoujian Zang, Qibin Wu,

Dongjiao Wang

и другие.

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Язык: Английский

Процитировано

0

Microbe-induced gene silencing of fungal gene confers efficient resistance against Fusarium graminearum in maize DOI Creative Commons
Ting Chen, Wen Tian,

Qing Shuai

и другие.

aBIOTECH, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Язык: Английский

Процитировано

0