Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development

Plant Methods, Год журнала: 2024, Номер 20(1)

Опубликована: Фев. 14, 2024

Abstract Background The challenge of pigeonpea breeding lies in its photosensitivity and seasonal specificity. This poses a problem to the breeder, as it restricts single generation advancement year. Currently, cross cultivar gap is twelve thirteen years resulting limited number varietal releases over past six decades. Shortening cycle was need hour, unlikely achieved by conventional breeding. To overcome these hindrances speed necessary leap. An experiment planned optimize coupled with seed descent or pod chip-based genotyping shorten at ICRISAT, Hyderabad. Monitored photoperiod, light wavelength, temperature crop management regime were indicators attributing success Result A photoperiod 13 h: 8 h vegetative: flowering filling stages ideal for shortening cycle. Broad spectrum (5700 K LED) hastened early vegetative growth formation. Whereas far-red (735 nm) favoured flowering. significant difference between photoperiods, genotypes well x genotype interaction both days plant height noted. Conclusion optimized protocol serves road map rapid pigeonpea. Deploying this protocol, possible advance 2–4 generations per can be reduced which otherwise takes 7 under Single Seed Descent marker assisted selection, strengthened precision technique aiding high throughput line development.

Язык: Английский

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Strong culm: a crucial trait for developing next-generation climate-resilient rice lines

Physiology and Molecular Biology of Plants, Год журнала: 2024, Номер 30(4), С. 665 - 686

Опубликована: Апрель 1, 2024

Язык: Английский

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The speed breeding technology of five generations per year in cotton

Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)

Опубликована: Март 19, 2025

Язык: Английский

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Speed Breeding: A Budding Technique to Improve Crop Plants for Multistress Tolerance

Опубликована: Янв. 1, 2025

Язык: Английский

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Rapid Ragi: A speed breeding protocol for Finger millet

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Background: Climate change is gradually increasing demand for resilient, nutritious crops like finger millet or ragi. Ensuring food security requires researchers to develop improved and adapted cultivars rapidly. Modern techniques such as genomics-assisted breeding have emerged in the previous decade combined with rapid generation advancement they will offer a step speed of cultivar development. Results: In this study, we developed repeatable cost-effective protocol by modulating agronomic physiological components early advancement. A photoperiod 9-hours, 29±2℃ temperature, 70% relative humidity, 105 plants per 1.5 sq. ft., 0.17% Hoagland’s No. 2 solution spray, restricted irrigation harvesting at maturity successfully reduced 28-54 days across groups millet. The advantage was validated segregating populations confirming up 4-5 generations year, instead 1-2 under field conditions. Conclusion: reduces cycle time significantly allowing increased genetic gain. provides development recombinant inbred lines (RILs), high-throughput phenotyping biotic abiotic stresses, genotyping selections.

Язык: Английский

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Costing of the breeding operations for the national maize programs in Eastern and Southern Africa

Frontiers in Sustainable Food Systems, Год журнала: 2025, Номер 9

Опубликована: Апрель 23, 2025

The Genetic Innovation Initiative on Accelerated Breeding (ABI) of Consultative Group International Agricultural Research (CGIAR) has been supporting the costing breeding operations for CGIAR-National and Extension Systems-Small to Medium Enterprises (CGIAR-NARES-SME’s) crop networks. aim is help these programs accurately estimate operational costs, develop precise budgets, set appropriate service fees, choose best technologies increased genetic gains. are being guided in using University Queensland’s open-source tool (UQ-BPCT). This paper outlines strategy demonstrates tool’s utility data from national Uganda (NARO), Zambia (ZARI), Zimbabwe (DR&SS). Results show that percentage budgets allocated germplasm development ranged 25% (DR&SS) 52% with conventional methods 7 47 times more than doubled haploids. Costs trials varied, ZARI spending 14% DR&SS 51%. In one cycle, NARO released 5 hybrid varieties, 2, 1. can be optimized by implementing several strategies: adopting an Enterprise System, incorporating digital disease screening phenotyping, network-based procurement consumables, modern techniques like haploids, genomic selection, speed shorten cycles, training personnel efficient resource use.

Язык: Английский

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Unveiling Performance and Genetic Estimates of Tongil‐Type Rice Genotypes for Yield Improvement and Breeding

Advances in Agriculture, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Enhancing grain yield (GY) is critical to satisfying increasing global food demands. However, owing its complex genetic basis, selecting for alone often insufficient. A more effective strategy involves evaluating alongside associated traits. In this study, we assessed the genotypic performance and estimated parameters yield‐related traits in 25 rice genotypes using a 5 × simple lattice design with two replications during 2023 growing season. Analysis of variance revealed highly significant differences ( p < 0.001) days 50% flowering (D50FLW), plant height (PH), number filled grains per panicle (NFGPN), thousand‐grain weight (TGW), while tillers (NTP), productive (NPTP), GY hectare were at 0.05. contrast, length (PL) unfilled (NUGPN) not significant. Notably, was positively correlated PH r = 0.40, 0.05) NFGPN 0.43, 0.05). Among traits, exhibited highest coefficient variation (GCV) (19.13%), broad‐sense heritability (Hb) (64.24%), advance as percentage mean (GAM) (31.59%), marking it key target selection. TGW also demonstrated high (79.83% 82.15%, respectively) moderate (GA) (14.7% 16.43%). Principal component analysis (PCA) further identified major contributor overall variability. These findings provide valuable insights targeted selection development efficient breeding strategies.

Язык: Английский

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Low-Temperature and Light Pretreatment Interactively Promote Rapid Flowering, Early Ripening, and Yield Accumulation of Winter Wheat

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 4280 - 4280

Опубликована: Апрель 30, 2025

Exposing wheat (Triticum aestivum L.) seeds to a combination of light and low temperatures for 4-6 weeks, followed by transferring speed breeding (SB) conditions, has been demonstrated effectively reduce generation time in winter wheat. To reveal the underlying mechanisms accelerated advancement wheat, we investigated changes transcriptome subsequent responses plant growth, flowering germinated vernalized at 4 °C with white exposure (VL) or under dark conditions (VD) weeks before sowing, growth SB conditions. Germinated without vernalization were directly sown served as controls (Control). The results showed that, compared Control VD, VL significantly expedited vernalization, resulting early around 6 days ripening progeny 13 higher germination index vigor index. transcriptomic analysis revealed that differently expressed genes (DEGs) involved GA synthesis its signal transduction both participated light-induced rapid development MADS-box transcription factors, especially VRN-A1 MADS55, might play vital role light- low-temperature-induced flowering. Our stress importance lay groundwork further elucidating

Язык: Английский

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Speed breeding 3.0: mainstreaming light-driven plant breeding for sustainable genetic gains

Trends in biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

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Onerice breeding framework: An end‐to‐end system to develop better varieties faster

Crop Science, Год журнала: 2025, Номер 65(3)

Опубликована: Май 1, 2025

Abstract Breeding in the Consultative Group on International Agricultural Research (CGIAR) system is an intricate process that integrates contributions of market research, pre‐breeding, breeding, breeding operations, and seed systems. Therefore, a well‐defined framework critical for effective efficient operation program. The OneRice Framework developed at Rice Institute (IRRI) these components, from initial research to establish goals, creating strategies improved product design development, swiftly testing replacing products through represents cutting‐edge approach offers comprehensive guidance harnessing modern tools technologies, including genomic selection, speed sparse testing, so on. Additionally, outlines systematically integrating novel genetic variation into elite programs pre‐breeding efforts. It adaptable across different crops dynamic, allowing adjustments program based target objectives, resource availability, tools. end‐to‐end all components enhance gains develop disseminate better faster address food, nutrition, income security. Consequently, fundamental blueprint rice ( Oryza sativa ) crop breeding.

Язык: Английский

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