VqERF1B‐VqERF062‐VqNSTS2 transcriptional cascade enhances stilbene biosynthesis and resistance to powdery mildew in grapevine DOI Creative Commons
Chaohui Yan,

Wandi Liu,

Ruimin Li

и другие.

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Grapes, as one of the world's oldest economic crops, are severely affected by grape powdery mildew, causing significant losses. As a phytoalexin against stilbenes and their key synthetic gene, stilbene synthase (STS), highly sought after researchers. In our previous research, new VqNSTS2, was identified from Vitis quinquangularis accession 'Danfeng-2' through transcriptomic analysis. However, function molecular mechanism VqNSTS2 gene remain unknown. Here, characterization transient overexpression we demonstrated that its expression product, stilbenes, can be detected in model plant tobacco, which does not inherently contain STSs. After artificially inoculating transgenic Arabidopsis lines overexpressing with Erysiphe necator, it found actively moved to pathogen's haustorium responding pathogen, recognized enveloped haustorium, blocking infection invasion exhibited disease resistance. Furthermore, Agrobacterium-mediated stable promoted accumulation enhanced resistance V. vinifera susceptible cultivar 'Thompson Seedless' E. necator. Additionally, screening identification, transcription factor, VqERF062, directly bind DRE RAA motifs on ProVqNSTS2, positively regulating expression. Moreover, VqERF062 interacted VqERF1B promote addition forming homodimer itself. Taken together, findings reveal VqERF1B-VqERF062- module is required for necator providing insights into regulatory biosynthesis.

Язык: Английский

VqERF1B‐VqERF062‐VqNSTS2 transcriptional cascade enhances stilbene biosynthesis and resistance to powdery mildew in grapevine DOI Creative Commons
Chaohui Yan,

Wandi Liu,

Ruimin Li

и другие.

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Grapes, as one of the world's oldest economic crops, are severely affected by grape powdery mildew, causing significant losses. As a phytoalexin against stilbenes and their key synthetic gene, stilbene synthase (STS), highly sought after researchers. In our previous research, new VqNSTS2, was identified from Vitis quinquangularis accession 'Danfeng-2' through transcriptomic analysis. However, function molecular mechanism VqNSTS2 gene remain unknown. Here, characterization transient overexpression we demonstrated that its expression product, stilbenes, can be detected in model plant tobacco, which does not inherently contain STSs. After artificially inoculating transgenic Arabidopsis lines overexpressing with Erysiphe necator, it found actively moved to pathogen's haustorium responding pathogen, recognized enveloped haustorium, blocking infection invasion exhibited disease resistance. Furthermore, Agrobacterium-mediated stable promoted accumulation enhanced resistance V. vinifera susceptible cultivar 'Thompson Seedless' E. necator. Additionally, screening identification, transcription factor, VqERF062, directly bind DRE RAA motifs on ProVqNSTS2, positively regulating expression. Moreover, VqERF062 interacted VqERF1B promote addition forming homodimer itself. Taken together, findings reveal VqERF1B-VqERF062- module is required for necator providing insights into regulatory biosynthesis.

Язык: Английский

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