Physiological and molecular profiling unveils oat (Avena sativa L.) defense mechanisms against powdery mildew
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Май 8, 2025
Oat
powdery
mildew,
caused
by
Blumeria
graminis
f.
sp.
avenae
(Bga),
poses
a
serious
threat
to
oat
production,
yet
the
underlying
resistance
mechanisms
remain
largely
unclear.
In
this
study,
we
investigated
early-stage
defense
responses
in
resistant
(BY642)
and
susceptible
(BY119)
varieties
following
Bga
inoculation
using
cytological
observations,
physiological
hormonal
measurements,
transcriptomic
analysis.
Microscopy
revealed
that
penetrates
tissues
directly
through
epidermal
cells
rather
than
stomata.
BY642
exhibited
rapid
robust
characterized
reactive
oxygen
species
(ROS)
accumulation
hypersensitive
response,
tightly
regulated
an
efficient
antioxidant
system
prevent
cellular
damage.
Hormone
profiling
indicated
salicylic
acid
(SA)-dominated
signaling
pathway
BY642,
accompanied
suppression
of
jasmonic
(JA)
responses.
Transcriptome
showed
early
activation
photosynthesis-related
pathways,
metabolic
reprogramming,
immune-related
networks,
including
MAPK
cascades
WRKY
transcription
factors.
Hub
genes
such
as
AsGSTU6
AsWRKY50
were
identified
key
contributors
resistance.
These
findings
suggest
employs
coordinated
strategy
integrating
ROS
dynamics,
SA
signaling,
transcriptional
regulation,
providing
novel
insights
into
molecular
basis
mildew
potential
targets
for
breeding.
Язык: Английский
When the source of resistance can be considered effective - resistance to Blumeria graminis f. sp. avenae found in Avena sterilis L.
European Journal of Plant Pathology,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 8, 2025
Abstract
Powdery
mildew,
caused
by
Blumeria
graminis
DC.
f.
sp.
avenae
Em.
Marchal,
is
currently
one
of
the
most
serious
foliar
diseases
common
oats
worldwide,
leading
to
significant
grain
yield
losses.
So
far,
13
resistance
genes
powdery
mildew
have
been
identified
and
characterized
in
oats,
but
only
a
few
these
are
effective
against
existing
pathogen
races.
Therefore,
there
need
identify
characterize
new
sources
resistance.
Avena
sterilis
L.
as
promising
source
fungal
diseases.
However,
identifying
requires
more
extensive
research
using
larger
group
differentiated
isolates
B.
.
This
study
aimed
effectiveness
A.
genotypes.
The
focused
on
28
genotypes,
from
IPK
Genebank
collection,
which
were
known
for
their
high
level
screening
tests.
was
evaluated
host–pathogen
tests
55
different
collected
various
regions
Europe.
These
infection
patterns
genes.
In
initial
stage
investigation,
previously
selected
genotypes
tested
5
isolates,
resulting
selection
seven
resistant
Further
50
revealed
that
two
showed
very
effectiveness.
studies
indicate
should
be
with
variety
isolates.
Tests
based
2–3
suitable
preliminary
screening.
Язык: Английский