New estimates and synthesis of chromosome numbers, ploidy levels and genome size variation in Allium sect. Codonoprasum: advancing our understanding of the unresolved diversification and evolution of this section DOI Creative Commons
Lucie Kobrlová, Michaela Jandová, Kateřina Vojtěchová

и другие.

Botanical studies, Год журнала: 2024, Номер 65(1)

Опубликована: Дек. 24, 2024

Abstract Background The genus Allium is known for its high chromosomal variability, but most chromosome counts are based on a few individuals and genome size (GS) reports limited in certain taxonomic groups. This evident the sect. Codonoprasum , species-rich (> 150 species) taxonomically complex section with weak morphological differences between taxa, presence of polyploidy frequent misidentification taxa. Consequently, significant proportion older karyological may be unreliable GS data lacking majority species within section. study, using counting flow cytometry (FCM), provides first comprehensive detailed insight into variation number, polyploid frequency distribution, members, marking step towards understanding unresolved diversification evolution this group. Results We analysed 1578 from 316 populations 25 taxa reported DNA ploidy levels their GS, calibration 22 Five had multiple levels. First estimates were obtained 16 A review number DNA-ploidy 129 revealed that all have x = 8, except A. rupestre two series ( descending dysploidy 7), unique Diploid dominated (72.1%), while di- & (12.4%) exclusively (15.5%) less common. Ploidy diversity showed diploid eastern Mediterranean decreased west north, whereas only cytotypes or northern northwestern Europe. 4.1-fold was observed across 33 so far (2C 22.3–92.1 pg), mainly due to polyploidy, downsizing Intra-sectional suggests evolutionary relationships, intraspecific some indicate heterogeneity and/or historical migration patterns. Conclusions Our study advantages FCM as an effective tool detecting determining could additional character phylogenetic relationships

Язык: Английский

Spatial Distribution of Genetic, Ploidy, and Morphological Variation of the Edaphic Steno-Endemic Alyssum moellendorfianum (Brassicaceae) from the Western Balkans DOI Creative Commons
Jasna Hanjalić, Belma Kalamujić Stroil, Sonja Siljak‐Yakovlev

и другие.

Plants, Год журнала: 2025, Номер 14(2), С. 146 - 146

Опубликована: Янв. 7, 2025

Polyploidy is a powerful mechanism driving genetic, physiological, and phenotypic changes among cytotypes of the same species across both large small geographic scales. These can significantly shape population structure increase evolutionary adaptation potential cytotypes. Alyssum moellendorfianum, an edaphic steno-endemic with narrow distribution in Balkan Peninsula, serves as intriguing case study. We conducted comprehensive analysis genetic diversity species' range, employing array techniques (nuclear microsatellites, amplified fragment length polymorphisms, plastid DNA sequences), flow cytometry (FCM), morphometry, pollen analysis. The study reveals two lineages: spatially distributed diploid tetraploid Clear divergence between diploids tetraploids was shown by AFLP, while sequences confirmed private haplotypes each studied populations. Higher allelic richness following north-south pattern were documented compared to diploids, indicated nuclear microsatellites. Morphometric via principal component (PCA) canonical discriminant (CDA) did not reveal any Nonetheless, distinction size clearly observed. results suggest autopolyploid origin from ancestors. Despite fragmentation very these populations harbour high diversity, which would allow them remain stable if natural processes undisturbed.

Язык: Английский

Процитировано

0

Analysis of changes in morphological characters and drought resistance of tetraploid P. alba DOI Creative Commons

liu yuanfu,

Wang Xinyu,

Siyuan Li

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Abstract Artificial induction of polyploids is an effective technique for plant breeding and genetic improvement. Understanding the changes in morphology after polyploidization key to studying underlying physiological mechanisms polyploid development. We obtained a tetraploid P. alba using colchicine performed characterization analysis on it. The results showed that height leaf area were smaller than those diploid plant. have thicker leaves, higher chlorophyll contents, larger but less dense stomata. Tetraploidization also resulted significant stem anatomy, including xylem width phloem width. In addition, we found plants exhibited enhanced drought tolerance compared with parent. our study not only revealed structural plants, provided valuable insights into alba.

Язык: Английский

Процитировано

0

Disentangling relationships in Euphorbia agraria s.l. (Euphorbiaceae) in south‐east Europe: One or two species? DOI Creative Commons
Naim Berisha, Peter Schönswetter, Božo Frajman

и другие.

Taxon, Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

Abstract We explored the diversification of south‐eastern and eastern European Euphorbia agraria s.l. applying molecular (amplified fragment length polymorphism [AFLP] fingerprinting sequencing nuclear ribosomal internal transcribed spacer [ITS]), cytogenetic (relative genome size [RGS] estimations) morphometric methods. The AFLP data, to a lesser extent ITS sequences, inferred two main phylogenetic lineages corresponding Balkan‐Pontic E. central Balkan subhastata ; their closest relatives are tommasiniana endemic north‐westernmost Peninsula salicifolia , which is more widespread in Peninsula. Genetic divergence reflected morphological ecological differentiation, rendering recognition – previously segregated as variety at species level, resulting revised taxonomic treatment. thrives grasslands ruderal places that lowlands adjacent Pontic region; continuity habitat probably confers weak genetic differentiation within this species. On other hand, grows screes open forests river gorges separated by mountain ridges, leading pronounced inter‐population differentiation. RGS data revealed di‐ tetraploid populations both combination with results suggest recurrent autopolyploidisation. Our support hotspot diversity indicate future biodiversity research part Europe should focus on central, southern were largely neglected previous studies.

Язык: Английский

Процитировано

0

Out of Liguria: how polyploidy affected diversification of the sweet spurge (Euphorbia dulcis, Euphorbiaceae), European widespread forest species DOI Creative Commons

Marija Kravanja,

Jasna Dolenc Koce, Božo Frajman

и другие.

Perspectives in Plant Ecology Evolution and Systematics, Год журнала: 2025, Номер unknown, С. 125861 - 125861

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

New estimates and synthesis of chromosome number, ploidy level and genome size variation in Allium sect. Codonoprasum: a step towards understanding the hitherto unresolved diversification and evolution of the section DOI
Lucie Kobrlová, Michaela Jandová, Kateřina Vojtěchová

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 9, 2024

Abstract Background The genus Allium is known for its high chromosomal variability, but most chromosome counts are based on a few individuals and genome size (GS) reports limited in certain taxonomic groups. This evident the sect. Codonoprasum, species-rich (> 150 species) taxonomically complex section with weak morphological differences between taxa, presence of polyploidy frequent misidentification taxa. Consequently, significant proportion older karyological may be unreliable GS data lacking majority species within section. study, using counting flow cytometry (FCM), provides first detailed insight into variation number, polyploid frequency distribution, members, step towards understanding section's unresolved diversification evolution. Results We analysed 1,582 from 311 populations 25 taxa reported DNA ploidy levels their GS, calibration 21 Five had multiple levels. estimates 16 primary estimates. A comprehensive review number DNA-ploidy 128 revealed that all x = 8, except A. rupestre two series (x descending dysploidy 7), unique this Diploid dominated (71.1%), while di-/polyploid (12.5%) pure (16.4%) were less common. Ploidy diversity showed diploid dominant eastern Mediterranean 85%), decreasing west north, only present northern northwestern Europe. 4.1-fold was observed across 33 (2C 22.3–92.1 pg), mainly due to polyploidy, downsizing Intra-sectional suggests evolutionary relationships, intraspecific some indicate heterogeneity and/or historical migration patterns. Conclusions Our study advantages FCM as an effective tool detecting determining could additional character evolution phylogenetic relationships

Язык: Английский

Процитировано

0

New estimates and synthesis of chromosome numbers, ploidy levels and genome size variation in Allium sect. Codonoprasum: advancing our understanding of the unresolved diversification and evolution of this section DOI Creative Commons
Lucie Kobrlová, Michaela Jandová, Kateřina Vojtěchová

и другие.

Botanical studies, Год журнала: 2024, Номер 65(1)

Опубликована: Дек. 24, 2024

Abstract Background The genus Allium is known for its high chromosomal variability, but most chromosome counts are based on a few individuals and genome size (GS) reports limited in certain taxonomic groups. This evident the sect. Codonoprasum , species-rich (> 150 species) taxonomically complex section with weak morphological differences between taxa, presence of polyploidy frequent misidentification taxa. Consequently, significant proportion older karyological may be unreliable GS data lacking majority species within section. study, using counting flow cytometry (FCM), provides first comprehensive detailed insight into variation number, polyploid frequency distribution, members, marking step towards understanding unresolved diversification evolution this group. Results We analysed 1578 from 316 populations 25 taxa reported DNA ploidy levels their GS, calibration 22 Five had multiple levels. First estimates were obtained 16 A review number DNA-ploidy 129 revealed that all have x = 8, except A. rupestre two series ( descending dysploidy 7), unique Diploid dominated (72.1%), while di- & (12.4%) exclusively (15.5%) less common. Ploidy diversity showed diploid eastern Mediterranean decreased west north, whereas only cytotypes or northern northwestern Europe. 4.1-fold was observed across 33 so far (2C 22.3–92.1 pg), mainly due to polyploidy, downsizing Intra-sectional suggests evolutionary relationships, intraspecific some indicate heterogeneity and/or historical migration patterns. Conclusions Our study advantages FCM as an effective tool detecting determining could additional character phylogenetic relationships

Язык: Английский

Процитировано

0