Cellular Mechanical Phenotypes of Drought-Resistant and Drought-Sensitive Rice Species Distinguished by Double-Resonator Piezoelectric Cytometry Biosensors

Biosensors, Год журнала: 2025, Номер 15(6), С. 334 - 334

Опубликована: Май 23, 2025

Various high-throughput screening methods have been developed to explore plant phenotypes, primarily at the organ and whole levels. There is a need develop phenomics cellular level narrow down genotype phenotype gap. This study used double-resonator piezoelectric cytometry biosensors capture dynamic changes in mechanical phenotypes of living cells two rice species, drought-resistant Lvhan No. 1 drought-sensitive 6527, under PEG6000 drought stress. In mechanomics parameters, including cell-generated surface stress (ΔS) viscoelastic parameters (G′, G″, G″/G′), were measured compared 5–25% PEG6000. showed larger but smaller with same concentration Moreover, better wall–plasma membrane–cytoskeleton continuum structure maintaining ability stress, as proven by transient tension (ΔS > 0) linear G′~ΔS, G″~ΔS relations higher 15–25% PEG6000, not for 6527 cells. Additionally, distinct defense resistance mechanisms identified through G″/G′ responses: (i) hardening followed softening recovery weak drought, (ii) strong drought. The abilities both recover from are than those Overall, phenotypic patterns (ΔS, G′, G″/G′, G″~ΔS) verified that has which consistent field data.

Язык: Английский

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Carex rigescens caffeic acid O-methyltransferase gene CrCOMT confer melatonin-mediated drought tolerance in transgenic tobacco

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Авг. 10, 2022

Melatonin is an important, multifunctional protective agent against a variety of abiotic and biotic stressors in plants. Caffeic acid O-methyltransferase (COMT) catalyzes the last step melatonin synthesis plants reportedly participates regulation stress response tolerance. However, few studies have reported its function melatonin-mediated drought resistance. In this study, CrCOMT was identified strongly induced by Carex rigescens. overexpression transgenic tobacco increased tolerance to with high levels seed germination, relative water content, survival rates. improved membrane stability, exhibited lower electrolytic leakage malondialdehyde as well higher photochemical efficiency than wildtype (WT) under stress. The also had proline accumulation antioxidant enzyme activity, which decreased oxidative damage due reactive oxygen species (ROS) hyperaccumulation transcription ROS scavenging genes significantly WT addition, content conditions. Exogenous applied C. rigescens confirm mediating tolerance; were higher, melatonin-treated untreated summary, these results show that plays positive role plant regulating endogenous content.

Язык: Английский

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Drought stress mitigation by foliar application of L-carnitine and its effect on radish morphophysiology

Physiology and Molecular Biology of Plants, Год журнала: 2023, Номер 29(4), С. 579 - 590

Опубликована: Апрель 1, 2023

Язык: Английский

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Ectopic Expression of AetPGL from Aegilops tauschii Enhances Cadmium Tolerance and Accumulation Capacity in Arabidopsis thaliana

Plants, Год журнала: 2024, Номер 13(17), С. 2370 - 2370

Опубликована: Авг. 25, 2024

Cadmium (Cd) is a toxic heavy metal that accumulates in plants, negatively affecting their physiological processes, growth, and development, poses threat to human health through the food chain. 6-phosphogluconolactonase (PGL) key enzyme Oxidative Pentose Phosphate Pathway(OPPP) plant cells, essential for cellular metabolism. The OPPP pathway provides energy raw materials organisms involved antioxidant reactions, lipid metabolism, DNA synthesis. This study describes Cd responsive gene

Язык: Английский

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ZmDnaJ-ZmNCED6 module positively regulates drought tolerance via modulating stomatal closure in maize

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 218, С. 109286 - 109286

Опубликована: Ноя. 8, 2024

Язык: Английский

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