Transcriptome Sequencing and Metabolome Analysis Reveals the Regulatory and Molecular Mechanism of Grain Filling Rate in Foxtail Millet (<em>Setaria italica L.</em>)

Опубликована: Апрель 18, 2024

The grain filling rate (GFR) plays a crucial role in determining yield. However, the regulatory and molecular mechanism of foxtail millet remains unclear. In this study, we found GFR &#039;Changnong No.47&#039; (CN47) was significantly higher at 14 days after flowering (DAF) 21 DAF comparison to &#039;Changsheng 13&#039; (CS13). Furthermore, CN47 also exhibited thousand weight yield than CS13. Therefore, conducted transcriptomics metabolomics analyze biological processes functional genes associated with during two stages both cultivars. A total 765 differentially expressed (DEGs) 246 accumulated metabolites (DAMs) were identified stage, while 908 DEGs 268 DAMs identified. integrated analysis co-mapped revealed enriched pathways, including flavonoid biosynthesis, plant hormone signal transduction, tyrosine metabolism, ATP-binding cassette (ABC) transporters, beta-alanine as well stilbenoid, diarylheptanoid gingerol biosynthesis. order elucidate their potential functions context GFR, developed gene-metabolite network for these metabolic pathways. Notably some ABC transporters transduction pathway implicated auxin transport highlighting filling. results provide initial insights into mechanisms underlying millet, offer valuable genetic resources further elucidation future studies. findings have established theoretical basis improving efficiency breeding millet.

Язык: Английский

Transcriptome Sequencing and Metabolome Analysis Reveals the Regulatory and Molecular Mechanisms of the Grain Filling Rate in Foxtail Millet (Setaria italica L.)

Agronomy, Год журнала: 2024, Номер 14(6), С. 1114 - 1114

Опубликована: Май 23, 2024

The grain filling rate (GFR) plays a crucial role in determining yield. However, the regulatory and molecular mechanisms of foxtail millet remains unclear. In this study, we found that GFR ′Changnong No.47′ (CN47) was significantly higher at 14 DAF (days after flowering) 21 comparison to ‘Changsheng 13’ (CS13). Furthermore, CN47 also exhibited thousand-grain weight yield than CS13. Therefore, RNA-seq UHPLC-MS/MS were used conduct transcriptome metabolome analyses during two stages both cultivars. Conjoint analysis transcriptomics metabolomics adopted order analyze biological processes functional genes associated with GFR. results identified total 765 differentially expressed (DEGs) 246 accumulated metabolites (DAMs) stage, while 908 DEGs 268 DAMs identified. integrated co-mapped revealed enriched pathways, including flavonoid biosynthesis, plant hormone signal transduction, tyrosine metabolism, ATP-binding cassette (ABC) transporters, beta-Alanine as well stilbenoid, diarylheptanoid, gingerol biosynthesis. elucidate their potential functions context GFR, developed gene–metabolite network for these metabolic pathways. Notably, some ABC transporters transduction pathway implicated auxin transport highlighting filling. These findings provide initial insights into underlying millet, offering valuable genetic resources further elucidation future studies. have established theoretical basis improving efficiency breeding millet.

Язык: Английский