WRKY27-SPDS1 module of Ichang papeda (Citrus ichangensis) promotes cold tolerance by modulating spermidine content

Horticulture Research, Год журнала: 2025, Номер 12(6)

Опубликована: Март 4, 2025

Abstract Spermidine (Spd) is one of the predominant polyamines in higher plants and plays a crucial role combating various abiotic stresses. However, molecular functions underlying regulatory mechanisms associated with plant Spd synthase (SPDS) genes cold tolerance remain poorly understood. In this study, treatment markedly induced accumulation enhanced SPDS activity Ichang papeda (Citrus ichangensis), cold-hardy Citrus genus. Exogenous supply led to dramatically improved tolerance. Two (CiSPDS1 CiSPDS2) were identified papeda, but only CiSPDS1 was substantially upregulated by cold. Overexpressing both tobacco (Nicotiana tabacum) lemon limon), cold-sensitive species, promoted synthesis transgenic plants. contrast, knockdown virus-induced gene silencing (VIGS) repressed greatly impaired tolerance, which restored exogenous replenishment Spd. addition, we demonstrated that WRKY27 (CiWRKY27) directly bound activated promoter through interacting W-box cis-acting element. Meanwhile, VIGS-mediated CiWRKY27 resulted marked reduction transcript levels contents significantly papeda. Taken together, study illustrated it as direct target CiWRKY27. These findings provide insight into mechanism module CiWRKY27-CiSPDS1 regulates for modulation

Язык: Английский

---

Transcription factor CsNAC25 mediating dual roles in tea plant secondary cell wall formation and trichome development

Plant Science, Год журнала: 2025, Номер 356, С. 112499 - 112499

Опубликована: Апрель 7, 2025

Язык: Английский

---

Functional Identification of PavRAP2.3 Highlights the Involvement in Regulation of Sweet Cherry Fruit Abscission

Physiologia Plantarum, Год журнала: 2025, Номер 177(3)

Опубликована: Май 1, 2025

ABSTRACT APETALA2/ethylene response factor (AP2/ERF) transcription factors constitute a significant family of that play crucial roles in plant growth and development. Meanwhile, organ abscission is common the development fruit trees. In particular, economically important sweet cherry ( Prunus avium L.) highly affected by excessive abscission, limiting its yield economic benefits. Previous research identified potential involvement PavRAP2.3 RELATED TO AP2.3 ) AP2/ERF based on significantly higher expression levels abscised tissues. This study found highest level mature fruits increased leaves treated with abscisic acid (ABA) ethephon (ETH). was localized nucleus exhibited transcriptional activity Y2H gold yeast. Yeast one‐hybrid assays dual‐luciferase revealed induced regulating PavCEL9‐like, gene associated cell wall reconstruction. Furthermore, ‐overexpressing Arabidopsis thaliana promoted petal which attributed to upregulation Cellulase 3 CEL3 ), Mannase 7 MAN7 ABA Insensitive 5 ABI5 ACC SYNTHASE6 ACS6 genes. Therefore, functions as positive regulator involved chiefly via PavCEL9‐like. These findings could facilitate breeding efforts aimed at reducing cherry, well better understanding function family.

Язык: Английский

---

CpSPL10-CpELF4 module involves in the negative regulation of flower bud differentiation in Chinese cherry

International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 135964 - 135964

Опубликована: Сен. 1, 2024

Язык: Английский

---

Heat-induced HpbHLH43 involves in promoting floral bud induction via activating HpSOC1 in pitaya

Scientia Horticulturae, Год журнала: 2024, Номер 338, С. 113773 - 113773

Опубликована: Ноя. 8, 2024

Язык: Английский

---