Genome-Wide Identification of the PR-1 Gene Family in Pyrus betulaefolia Bunge and Its Expression Analysis Under Fire Blight Stress DOI Open Access

Abudusufuer Wufuerjiang,

Jingyi Sai,

Yue Wen

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(11), С. 5074 - 5074

Опубликована: Май 24, 2025

Fire blight, caused by Erwinia amylovora, is a devastating bacterial disease threatening apple, pear, and other Rosaceae species. In our prior study, transcriptome analysis identified fire blight-resistant variety, Duli (Pyrus betulifolia Bunge), highlighted the PR1 gene as key resistance factor. Using Duli’s genomic data, we systematically characterized Pb-PR-1 family through bioinformatics analysis. A total of 31 genes were found, encoding proteins 123–341 amino acids. Phylogenetic grouped these into four subfamilies, with 27 distributed across seven chromosomes, all contain conserved CAP superfamily domain. Their promoter regions enriched in hormone stress-responsive elements. After inoculation E. susceptible showed lesion development day 2, rapid progression, while resistant plants exhibited slower advancement smaller lesions. Enzyme activity assays revealed that plants, PPO (polyphenol oxidase) CAT (catalase) activities peaked on 6, showing 2.4-fold 3.81-fold increase compared to Duli. At same time, MDA (malondialdehyde) content decreased 16.6%. The SOD (superoxide dismutase) PAL (phenylalanine ammonia-lyase) 4, increments 34.32% 47.1% over qRT-PCR significant differences expression between post-inoculation. Notably, Pb-PR-1-11, Pb-PR-1-21, Pb-PR-1-26 increased infection duration, aligning trends. Other high early but declined 6. Pb-PR-1-3, Pb-PR-1-6, Pb-PR-1-8, Pb-PR-1-16, Pb-PR-1-30 upregulated 13.17-fold average 2. summary, elevated during enhanced defense-related enzyme activities, improving plant resistance. This study provides foundation for understanding PR-1 family’s role advancing blight Pyrus

Язык: Английский

Genome-Wide Identification of the PR-1 Gene Family in Pyrus betulaefolia Bunge and Its Expression Analysis Under Fire Blight Stress DOI Open Access

Abudusufuer Wufuerjiang,

Jingyi Sai,

Yue Wen

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(11), С. 5074 - 5074

Опубликована: Май 24, 2025

Fire blight, caused by Erwinia amylovora, is a devastating bacterial disease threatening apple, pear, and other Rosaceae species. In our prior study, transcriptome analysis identified fire blight-resistant variety, Duli (Pyrus betulifolia Bunge), highlighted the PR1 gene as key resistance factor. Using Duli’s genomic data, we systematically characterized Pb-PR-1 family through bioinformatics analysis. A total of 31 genes were found, encoding proteins 123–341 amino acids. Phylogenetic grouped these into four subfamilies, with 27 distributed across seven chromosomes, all contain conserved CAP superfamily domain. Their promoter regions enriched in hormone stress-responsive elements. After inoculation E. susceptible showed lesion development day 2, rapid progression, while resistant plants exhibited slower advancement smaller lesions. Enzyme activity assays revealed that plants, PPO (polyphenol oxidase) CAT (catalase) activities peaked on 6, showing 2.4-fold 3.81-fold increase compared to Duli. At same time, MDA (malondialdehyde) content decreased 16.6%. The SOD (superoxide dismutase) PAL (phenylalanine ammonia-lyase) 4, increments 34.32% 47.1% over qRT-PCR significant differences expression between post-inoculation. Notably, Pb-PR-1-11, Pb-PR-1-21, Pb-PR-1-26 increased infection duration, aligning trends. Other high early but declined 6. Pb-PR-1-3, Pb-PR-1-6, Pb-PR-1-8, Pb-PR-1-16, Pb-PR-1-30 upregulated 13.17-fold average 2. summary, elevated during enhanced defense-related enzyme activities, improving plant resistance. This study provides foundation for understanding PR-1 family’s role advancing blight Pyrus

Язык: Английский

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