Do guard cells have single or multiple defense mechanisms in response to flg22? DOI Creative Commons
Zalán Czékus, András Kukri, Atina Martics

и другие.

Physiologia Plantarum, Год журнала: 2025, Номер 177(3)

Опубликована: Май 1, 2025

Abstract Bacterial flagellin (flg22) induces rapid and permanent stomatal closure. However, its local systemic as well tissue‐ cell‐specific effects are less understood. Our results show that flg22 induced closure in intact tomato plants, which was regulated by reactive oxygen‐ nitrogen species, also affected the photosynthetic activity of guard cells but not mesophyll cells. Interestingly, extensive expression Ethylene response factor 1 observed after exposure to flg22, whereas relative transcript levels Defensin increased only six hours, especially leaves. Following ethylene emission already one jasmonic acid leaves hours treatment. Using immunohistochemical methods, significant defensin accumulation found epidermis stomata flg22‐treated above them. Immunogold labelling revealed defensins cell wall parenchyma Furthermore, single qRT‐PCR confirmed able synthesise defensins. It can be concluded involved first line plant defense regulating pore size, defend themselves producing accumulating antimicrobial where phytopathogens penetrate.

Язык: Английский

Do guard cells have single or multiple defense mechanisms in response to flg22? DOI Creative Commons
Zalán Czékus, András Kukri, Atina Martics

и другие.

Physiologia Plantarum, Год журнала: 2025, Номер 177(3)

Опубликована: Май 1, 2025

Abstract Bacterial flagellin (flg22) induces rapid and permanent stomatal closure. However, its local systemic as well tissue‐ cell‐specific effects are less understood. Our results show that flg22 induced closure in intact tomato plants, which was regulated by reactive oxygen‐ nitrogen species, also affected the photosynthetic activity of guard cells but not mesophyll cells. Interestingly, extensive expression Ethylene response factor 1 observed after exposure to flg22, whereas relative transcript levels Defensin increased only six hours, especially leaves. Following ethylene emission already one jasmonic acid leaves hours treatment. Using immunohistochemical methods, significant defensin accumulation found epidermis stomata flg22‐treated above them. Immunogold labelling revealed defensins cell wall parenchyma Furthermore, single qRT‐PCR confirmed able synthesise defensins. It can be concluded involved first line plant defense regulating pore size, defend themselves producing accumulating antimicrobial where phytopathogens penetrate.

Язык: Английский

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