The TACOS Technique: A Stepwise Protocol for Alveolar Ridge Augmentation Using Customized Titanium Mesh

Medicina, Год журнала: 2025, Номер 61(1), С. 58 - 58

Опубликована: Янв. 2, 2025

Background: Alveolar ridge resorption following tooth loss poses a significant challenge for successful dental implant placement. In cases of severe atrophy, bone augmentation is required to restore sufficient volume. This technical note outlines detailed, stepwise surgical protocol horizontal and vertical alveolar using customized titanium mesh. Materials Methods: The procedure includes precise mesh fitting, autologous grafting, the application bioactive agents promote regeneration. Emphasis placed on technique’s feasibility, predictability, critical steps necessary preventing complications. Results: use ensures stability improved regeneration outcomes, enabling clinicians achieve placement even in severely atrophic ridges. Conclusions: described has demonstrated predictable results both clinical radiographic evaluations, offering an effective solution complex cases.

Язык: Английский

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RNAseq of Gingival Fibroblasts Exposed to PRF Membrane Lysates and PRF Serum

Cells, Год журнала: 2024, Номер 13(15), С. 1308 - 1308

Опубликована: Авг. 5, 2024

Platelet-rich fibrin (PRF) is prepared by spontaneous coagulation of fractionated blood. When squeezed between two plates, PRF separated into solid membranes and a liquid exudate, the serum. The question arises regarding how much overall activity remains in what discarded To this end, we have exposed gingival fibroblasts to lysates from serum, followed bulk RNA sequencing. A total 268 up- 136 down-regulated genes membrane were significantly regulated under premise minimum log2 with 2.5-fold change minus log10 significance level two, respectively. serum only caused 62 32 these conditions. Among 46 commonly up-regulated CXCL1, CXCL5, CXCL6, CXCL8, IL33, IL6, PTGS2/COX2, stanniocalcin-1—all linked an inflammatory response. further increased chemokines CCL2, CCL7, CXCL2, CXCL3, IL1R1, IL1RL1, IL1RN, as well paracrine factors IL11, LIF, IGF1, BMP2, BMP6, FGF2, CCN2/CTGF, all hyaluronan synthases. On other hand, DKK1. included interferon-induced protein tetratricopeptide repeats (IFIT1, IFIT2, IFIT3) odd-skipped-related transcription (OSR1 OSR2), FGF18 GDF15, Taken together, lysates, compared cause more complex response fibroblasts, but each chemokine expression fibroblasts.

Язык: Английский

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Autogenous platelet concentrates for treatment of intrabony defects—A systematic review with meta‐analysis

Periodontology 2000, Год журнала: 2024, Номер unknown

Опубликована: Окт. 19, 2024

Abstract To provide an overview of the use autogenous platelet concentrates (APCs) in periodontal regeneration and to conduct a systematic review (SR) treatment outcomes intrabony defects by using platelet‐rich fibrin (PRF) compared with other commonly utilized modalities. The eligibility criteria comprised randomized controlled trials (RCTs) comparing clinical PRF that Studies were classified into 21 categories five different groups as follows: Group I (1) open flap debridement (OFD) alone versus OFD/PRF, (2) OFD Titanium‐PRF (T‐PRF) II, (3) Comparative protocols (PRF vs. T‐PRF), III (Comparative PRF): (4) OFD/PRP (5) OFD/bone graft(BG)/PRGF OFD/BG/PRF, (6) OFD/EMD (7) OFD/BG/EMD (8) OFD/collagen membrane (CM) (9) OFD/BG/BM (10) OFD/BG IV (Addition groups) (11) (12) OFD/GTR + (13) OFD/EMD/PRF (14) OFD/BG/BM/PRF, V Biomaterial/Biomolecule OFD/PRF … (15) OFD/PRF/BG, (16) OFD/PRF/antibiotic, (17) OFD/PRF/Metformin, (18) OFD/PRF/Bisphosphonates, (19) OFD/PRF/Statins, (20) OFD/BG/PRF OFD/BG/PRF/Statins, (21) OFD/PRF/low‐level laser therapy (LLLT). Weighted means forest plots calculated for probing pocket depth (PPD), attachment level (CAL), radiographic bone fill (RBF). From 596 records identified, 55 RCTs included. I: statistically significantly reduced PPD improved CAL RBF when OFD. II: A significant difference between various was only observed PPD. III: No advantage found following groups: OFD/PRP, OFD/EMD, OFD/BM, or OFD/BG. IV: addition led improvements PPD, alone. V: either BG well three biomolecules (metformin, bisphosphonates, statins) CAL, and/or Similar results OFD/BG, OFD/BM. grafting material small may offer additional advantages, thus warranting further investigations. Future research investigating PRF, longer‐term outcomes, at human histological remains needed.

Язык: Английский

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Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model

Bioengineering, Год журнала: 2024, Номер 11(5), С. 414 - 414

Опубликована: Апрель 23, 2024

Platelet-rich fibrin, the coagulated plasma fraction of blood, is commonly used to support natural healing in clinical applications. The rat calvaria defect a standardized model study bone regeneration. It remains, however, unclear if appropriate investigate impact human PRF (Platelet-Rich Fibrin) on To this end, we soaked Bio-Gide® collagen membranes or liquid concentrated before placing them onto 5 mm calvarial defects Sprague Dawley rats. Three weeks later, histology and micro-computed tomography (μCT) were performed. We observed that with show characteristic features new areas mineralized matrix, indicated by median volume 1.5 mm3 (range: 0.9; 5.3 mm3). Histology revealed growing underneath membrane hybrid where fibers are embedded bone. Moreover, passive mineralization observed. PRF, devoid histological formation center defect; only occasionally, formed at margins. Human (h-PRF) caused 0.9 0.3-3.3 mm3), which was significantly lower than what (r-PRF), BV 1.2 0.3-5.9 Our findings indicate suitable for assessing effects formation, but caution warranted when extrapolating conclusions regarding efficacy PRF.

Язык: Английский

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The effect of autologous platelet concentrates as solely grafting material or with bone graft materials in maxillary sinus augmentation: a meta-analysis of randomized controlled trials

Clinical Oral Investigations, Год журнала: 2025, Номер 29(2)

Опубликована: Фев. 7, 2025

Язык: Английский

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Platelet‐rich plasma (PRP) versus injectable platelet‐rich fibrin (i‐PRF): A systematic review across all fields of medicine

Periodontology 2000, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Abstract This systematic review aimed to evaluate all available evidence across fields of medicine regarding the comparative effectiveness platelet‐rich plasma (PRP) versus injectable fibrin (i‐PRF). A comprehensive search was conducted in PubMed, Scopus, and Web Science up September 30, 2024. Following a thorough screening process, studies were divided into vitro, vivo, clinical based on their tissue/clinical indications. The initial generated 2192 articles, which 23 met inclusion criteria. findings demonstrated that i‐PRF yielded higher platelet concentrations offered more sustained, long‐term release growth factors over time when compared PRP. Overall, it determined from vitro significantly improved activity many cell types, including for skin, cartilage, periodontal, bone, soft tissue around dental implants, pulp cells. In vivo outcomes also generally indicated outperformed PRP cartilage testicular regeneration. However, orthodontic tooth movement, found lead superior short‐term effects, while showed beneficial effects. Clinical skin regeneration, Outcomes movement utilizing or remain controversial. 72% studies, better various PRP, whereas 24% no differences between groups. Reasons inconsistencies may be attributed protocol types. additional are needed with well‐designed research centrifugation protocols understand regenerative potential concentrates medicine. offers sustained prolonged favored majority should, therefore, medical applications.

Язык: Английский

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Advantages of horizontal centrifugation of platelet‐rich fibrin in regenerative medicine and dentistry

Periodontology 2000, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

The aim of this comprehensive review was to evaluate comparative studies on horizontal and fixed-angle centrifugation methods for preparing platelet-rich fibrin (PRF). Furthermore, additional utilizing PRF (H-PRF) were systematically investigated. This overview article offers deeper insights into the advantages H-PRF when compared across a wide range regenerative medical dental applications. A search conducted in PubMed Web Science up December 5, 2024. Grey literature also searched via Google Scholar relevant studies, reference lists eligible screened further potential inclusion. All vitro, vivo, clinical that utilized or swing-out prepare solid liquid PRF, along with their subfractions such as buffy coat, platelet-poor plasma (PPP), heated variants like albumin gel (Alb-PRF) interventions, included study. total 75 included. Thirteen directly producing while remaining 62 non-comparative focused expanding uses applications H-PRF. These spanned categories cell concentrations, matrix structure, growth factor release, antibacterial anti-inflammatory properties, bone, periodontal, cartilage, skin, hair, endodontics, corneal defect repair, wound healing, soft tissue regeneration. Of comparing centrifugation, 84.6% favored 15.4% found no difference. None centrifugation. Additionally, more optimized concentrating liquid-PRF (C-PRF) using extending resorption properties PRF-ranging from 2 3 weeks membranes lasting 4 months through an denaturation process were-further discussed. Based these findings, it remains logical utilize practice owing greater superiority results majority studies. Nevertheless, are needed support findings. While current evidence is limited trials warranted, several have now indicated fixed-angle, higher uniform distribution, increased release. suggest use may lead enhanced outcomes application indicated. Since can better separation, should be preferred method C-PRF Alb-PRF

Язык: Английский

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Alveolar ridge augmentation in Oral and Maxillofacial Surgery: a study on current practices, patient management and innovations in Germany

International Journal of Implant Dentistry, Год журнала: 2025, Номер 11(1)

Опубликована: Апрель 16, 2025

Abstract Introduction This study identifies current practices, patient management concepts and innovations in alveolar ridge augmentation (ARA) Oral Maxillofacial Surgery (OMFS) Germany. Material methods A survey with a dynamic online questionnaire up to 40 questions was designed collect general specific data on ARA, such as case numbers, imaging, surgical techniques, (bio-)materials, OMFS. After internal external validation, 1863 OMF surgeons within the German Association for (DGMKG) were invited via email participate. Data anonymized descriptively. Results 324 participated (response rate 17.39%). Most participants (60.8%) work private practices without inpatient care. 62.03% of insert > 200 implants, 28.70% perform ARA annually. About 30.86% also provide implant-based prosthetic restorations. Cone-beam computed tomography (CBCT) is imaging method most preferred by 87.74% participants. The common techniques are sinus lift (SL) bone block (97.31%, 90.57%, 73.4%, respectively). Intraoral harvested autogenous grafts (ABG) commonly used (96.63%). oblique line participants’ donor site ABG (93.27%). xenogeneic substitutes frequently graft combinations (72.73%). Platelet-rich fibrin (PRF) 58.59% mostly SL procedures (76.44%). PRF substitute biofunctionalize biomaterials (48.16%). antibiotics 86.40% pre-/intraoperatively 88.97% postoperatively ARA. believe technique (94.49%) surgeon's experience (92.28%) critical factors success. 46.32% aim skeletal envelope. Conclusion findings highlight management, OMFS They show standard numerous variations several aspects. Clinical relevance Surgical technique, experience, health compliance relevant success factors. underlies importance extended training careful selection.

Язык: Английский

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Growth Factors Release in Concentrated Growth Factor and Advanced Platelet‐Rich Fibrin Sticky Bone

Oral Diseases, Год журнала: 2025, Номер unknown

Опубликована: Май 6, 2025

ABSTRACT Objective Sticky bone, created from concentrated growth factor (CGF) or advanced platelet‐rich fibrin (A‐PRF), contains various factors. This study analyzed the release patterns of factors CGF sticky bone (CGF‐SB) and A‐PRF (A‐PRF‐SB) to inform their application in grafting. Methods CGF, A‐PRF, CGF‐SB, A‐PRF‐SB were incubated for 28 days, with supernatant collected on days 1, 7, 14, 21, 28. Growth factors—platelet‐derived factor‐BB (PDGF‐BB), vascular endothelial (VEGF), basic fibroblast (bFGF)—were quantified using multiplex assays. Transforming factor‐β1 (TGF‐β1) insulin‐like factor‐1 (IGF‐1) measured by ELISA. Results released more than half PDGF‐BB, VEGF, bFGF within day followed rapid declines. Differently, these gradually, surpassing 50% cumulative 7 sustaining over time. IGF‐1 TGF‐β1 all groups reached respectively. significantly bFGF, CGF‐SB ( p < 0.05). Conclusions Both exhibit slower sustained compared alone, offering superior profiles, highlighting its enhanced potential grafting applications.

Язык: Английский

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In Vitro Evaluation of the Antibacterial Properties and Cellular Response of Liquid-Leukocyte Platelet-Rich Fibrin Products on Barrier Membranes: A Pilot Study

Dentistry Journal, Год журнала: 2025, Номер 13(6), С. 228 - 228

Опубликована: Май 22, 2025

Background: Barrier membranes (BMs) have been used in dental surgical procedures for decades, but their exposure can increase the risk of infections and compromise healing from regenerative procedures. Liquid-leukocyte platelet-rich fibrin (LPRF) products shown antimicrobial effects enhance wound healing. This vitro study aimed to evaluate cellular responses LPRF as adjunctive treatments barrier membranes, hypothesizing that two liquid could improve antibacterial activity against selected oral pathogen species augment human gingival fibroblast proliferation on BM. Methods: exudate (LPRF-EX) fibrinogen (PLyf), products, were prepared with recommended centrifugation protocols treat resorbable (Bio-gide®) non-resorbable (Cyto-plast™) BMs. Human fibroblasts (HGFs) cultured treated untreated Scanning electron microscopy (SEM) was applied observe cell adhesion, CCK-8 assays proliferation. Oral P. gingivalis A. naeslundii incubated Bacterial adhesion visualized using SEM, colony-forming unit (CFU) counts obtained. Results: SEM images showed markedly greater network formation after 7 days BM PLyF, no notable differences other or groups both treatments. non-significant HGF at 3 5 days. growth inhibition LPRF-EX- PLyf-treated BMs, greatest reduction CFU noted Cytoplast™. Conclusions: Within limitations this preliminary study, it be concluded LPRF-EX PLyf treatment induced an effect. Their response unclear due lack significant findings analysis.

Язык: Английский

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