Recent advances in low‐background luminescent nanomaterials‐enhanced lateral flow assays for clinical diagnosis

Deleted Journal, Год журнала: 2025, Номер unknown

Опубликована: Янв. 5, 2025

Abstract Lateral flow assays (LFAs) are widely applicable in clinical point‐of‐care testing (POCT) due to their unique advantages such as simplicity, rapidity, and cost‐effectiveness. However, sensitivity is often constrained by the background autofluorescence of biological sample, self‐matrix, or readout technique, thereby leading overlooking trace amounts biomarkers present early disease. In recent years, various nanomaterials‐based methods have been developed address this issue, including time‐gated wavelength‐differentiated strategy external modulated for separation minimize interference from samples. This review provides a comprehensive overview low‐background luminescent nanoparticles (LBLNPs)‐enhanced LFA systems, focusing on analyzing underlying mechanism these nanomaterials improving accuracy LFAs platform diagnosis. Representative examples selected demonstrate potential detecting disease‐associated samples blood, urine, saliva et al. Finally, unresolved challenges future development prospects briefly discussed.

Язык: Английский

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Introduction to the Diagnostics in Periodontology and Implant Dentistry issue

Periodontology 2000, Год журнала: 2024, Номер 95(1), С. 7 - 9

Опубликована: Июнь 1, 2024

Abstract Chronic inflammatory periodontal disease and its related condition, peri‐implant disease, are highly prevalent globally require accurate speedy diagnosis. The focus of this volume dedicated to diagnostics is cover modern enhancements in accuracy, simplicity speed. An international assortment experts has been tasked with reviewing defined areas current best practice as well innovation the field periodontitis peri‐implantitis diagnostics. Periodontitis irreversible, chronic, cumulative conditions propagated by bacteria host factors, which involve soft hard tissue changes, these changes measured diagnostic process. Clinically relevant modifications healthy state detected using clinical, radiological laboratory or point care testing, testing approaches critically reviewed at length state‐of‐the‐art resume

Язык: Английский

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Treponema denticola major surface protein (Msp): a key player in periodontal pathogenicity and immune evasion

Archives of Microbiology, Год журнала: 2025, Номер 207(2)

Опубликована: Янв. 18, 2025

Язык: Английский

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Advances in periodontal healing biomarkers

Advances in clinical chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Development and Evaluation of Gelatin-Based Gummy Jellies Enriched with Oregano Oil: Impact on Functional Properties and Controlled Release

Foods, Год журнала: 2025, Номер 14(3), С. 479 - 479

Опубликована: Фев. 2, 2025

Functional foods play a crucial role in contemporary dietary strategies. This study investigates the incorporation of oregano oil, bioactive extract that is known for its antimicrobial and antioxidant properties, into gelatin-based gummy jellies to develop functional food products with controlled release properties. The were evaluated mass uniformity, swelling index, disintegration time, tensile strength under simulated oral gastric conditions. results showed oil significantly reduced index (e.g., 128.76 ± 0.67% at pH 5) prolonged time 6–18 min across environments), highlighting potential release. mechanical remained stable (5.2 0.3 N), ensuring structural integrity. These findings suggest oregano-oil-enriched offer health benefits, although further studies are needed explore their long-term stability bioavailability.

Язык: Английский

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The EUCAST Disk Diffusion Method for Antimicrobial Susceptibility Testing of Oral Anaerobes

Apmis, Год журнала: 2025, Номер 133(2)

Опубликована: Фев. 1, 2025

There is a need for standardized methods antimicrobial susceptibility testing (AST) of anaerobic bacteria involved in oral and extra-oral infections. We tested the recently published EUCAST disk diffusion method rapidly growing anaerobes on selected anaerobes. AST 20 strains Prevotella spp., 11 Porphyromonas gingivalis, six Fusobacterium nucleatum complex was performed with amoxicillin metronidazole disks using guidelines. Plates were incubated anaerobically, inhibition zones evaluated after h (EUCAST recommendations) again 44 h. The recommended agar supported growth all 38 strains. Twenty-hour incubation sufficient assessment zone diameters Although approved Prevotella, an extended study species showed inconsistent within time limit some All P. gingivalis required evaluation zones. applicable to members complex. several needed enable reading diameter. Further studies are necessary validate prolonged slow-growing

Язык: Английский

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Advanced biomaterials for targeting mature biofilms in periodontitis therapy

Bioactive Materials, Год журнала: 2025, Номер 48, С. 474 - 492

Опубликована: Фев. 27, 2025

Язык: Английский

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Oral Medicine and Oral Clinical Chemistry Game Changers for Future Plaque Control and Maintenance: PerioSafe® aMMP-8 POCT, Lumoral® 2× PDT- and Lingora® Fermented Lingonberry Oral Rinse-Treatments

Dentistry Journal, Год журнала: 2025, Номер 13(3), С. 127 - 127

Опубликована: Март 13, 2025

Background: Periodontitis is a global health crisis that affects almost half of the world’s population and commonly goes unnoticed because its asymptomatic pain-free nature. For early easy detection treatment, safe non-invasive chair-side oral fluid biomarker (aMMP-8) diagnostics new anti-microbial, anti-inflammatory anti-proteolytic treatment modalities have been developed, which this review aims to introduce. Methods: convenient diagnosis tackling periodontitis, adoption an aMMP-8 point-of-care rapid diagnostic test (POCT) has proposed, comparable home pregnancy COVID-19 antigen tests, be conveniently used by healthcare professionals patients themselves. To improve detected Finnish scientists also developed potentially industry-altering, biofilm-modulating, anti-inflammatory, (i) dual-light-activated photodynamic-therapy (2×PDT) (ii) fermented lingonberry juice (FLJ) rinse designed for personalized medicine professional use. These technologies are reviewed some unpublished results presented. Results: superior grade periodontitis (progression rate) when compared total latent/proform MMP-8 (total-MMP-8) microbial lipopolysaccharide (LPS/LAL) activity. Cut-off 20 ng/mL optimal cut-off POCT does not make false positives. Antibacterial 2× PDT light anti-microbial FLJ treatments can eliminate reduce problem-causing bacteria Candida-yeasts from mouth. Conclusions: shown promising could potential revolutionize diagnosis, prevention, care, plaque control maintenance. game-changer launched clinical field in dentistry: chemistry.

Язык: Английский

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The Global Burden of Periodontal Disease: A Narrative Review on Unveiling Socioeconomic and Health Challenges

International Journal of Environmental Research and Public Health, Год журнала: 2025, Номер 22(4), С. 624 - 624

Опубликована: Апрель 16, 2025

Periodontal disease is a prevalent chronic inflammatory condition that impacts over billion people worldwide, leading to substantial tooth loss, reduced quality of life, and heightened systemic health risks. This narrative review synthesizes current evidence regarding the global burden periodontal disease, its established associations with conditions including cardiovascular diabetes, adverse pregnancy outcomes, respiratory infections, neurodegenerative disorders, significant socioeconomic implications. The focused on following research question: What how do implications necessitate integrated public strategies? A structured search PubMed, Scopus, WHO databases from 2000 2024 was conducted identify relevant literature using key terms, “periodontal disease”, “global burden”, “systemic inflammation”, “public strategies”. Out 312 initially identified articles, 175 satisfied inclusion criteria for final synthesis. findings underscore significance as modifiable risk factor various noncommunicable diseases, influence healthcare disparities progression, critical necessity strategies mitigate consequences. concludes coordinated policy reform, system integration, enhanced efforts are crucial mitigating advancing equity.

Язык: Английский

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Microbiological and Salivary Biomarkers Successfully Predict Site-Specific and Whole-Mouth Outcomes of Nonsurgical Periodontal Treatment

Journal of Clinical Medicine, Год журнала: 2024, Номер 13(14), С. 4256 - 4256

Опубликована: Июль 21, 2024

Background/Objectives: Nonsurgical periodontal treatment (NSPT) is the gold-standard technique for treating periodontitis. However, an individual's susceptibility or inadequate removal of subgingival biofilms could lead to unfavorable responses NSPT. This study aimed assess potential salivary and microbiological biomarkers in predicting site-specific whole-mouth outcomes Methods: A total 68 periodontitis patients exhibiting 1111 pockets 4 6 mm depth completed active phase treatment. Clinical parameters, saliva, biofilm samples were collected from each patient at baseline three months after quantitative PCR assay was used detect presence Fusobaterium nucleatum Porphyromonas gingivalis samples. Salivary including matrix metalloproteinase (MMP)-9, glutathione S-transferase (GST), Annexin-1 assayed both qualitatively (Western blot analysis) quantitively (ELISA). Results: NSPT yielded significant improvements all clinical a reduction bacterial load decreased levels MMP-9 together with increased concentrations GST Annexin-1. The binary logistic regression suggested that overall accuracy P. identification, probing pocket depth, interproximal sites 71.1% successful outcomes. biomarker model 79.4% following Conclusions: At baseline, shallow interdental locations lower abundance predictive favorable response site level. Decreased associated can predict

Язык: Английский

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