Genome Manipulation Advances in Selected Aquaculture Organisms

Reviews in Aquaculture, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 15, 2024

ABSTRACT With the rising global demand for seafood and challenges posed by overfishing climate change, aquaculture sector has become increasingly important in providing high‐quality protein human consumption. Although traditional selection breeding programs have made great strides genetic improvement of species over past decades, faster more precise tools, such as genome manipulation, are needed performance enhancement stock. This review presents a comprehensive overview current status three major manipulation including RNA interference (RNAi), gene transfer, editing species, discusses advances made, faced, potential future directions this fast‐developing field. Taking catfish an example, paper reviews specific applications these techniques to improve traits growth, disease resistance, reproduction, nutritional profiles various commercially fishes crustaceans, highlighting successful ongoing research efforts. We also propose CRISPR/Cas9‐mediated multiplex knockout or replacement multiple genes parallel fish. Collectively, provides insights into evolving landscape sheds light on its implications sustainable practices responsible innovation.

Язык: Английский

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Editing the Melanocortin-4 Receptor Gene in Channel Catfish Using the CRISPR-Cas9 System

Fishes, Год журнала: 2023, Номер 8(2), С. 116 - 116

Опубликована: Фев. 18, 2023

The melanocortin-4 receptor (MC4R) plays a critical role in homeostasis and the regulation of body weight. Polymorphisms mc4r gene have been discovered linked to growth, carcass composition, meat quality traits. Therefore, we used CRISPR-Cas9 system target most important freshwater aquaculture species USA, channel catfish, Ictalurus punctatus. Guide RNAs were designed direct Cas9 coding sequence catfish gene. gRNA(s)-Cas9 mixtures delivered into one-cell embryos using electroporation microinjection. For each treatment, nature rate mutations analyzed. Hatching survival rates calculated. overall mutation 30.6% 66.7–90.6% for microinjection, respectively. Mutated fish generated via or microinjection exhibited 38% 20% improvement weight, respectively, when compared with full-sib control. mean feed conversion ratio mutants was 1.18 1.57 control fish. improved growth indicate that generation mc4r-edited could economically benefit production.

Язык: Английский

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The food safety assessment of all-female common carp (Cyprinus carpio) (cyp17a1+/−;XX genotype) generated using genome editing technology

Food and Chemical Toxicology, Год журнала: 2023, Номер 181, С. 114103 - 114103

Опубликована: Окт. 16, 2023

There are several technical challenges and public issues concerning genome editing applications before they become viable in commercial aquaculture. Recently, we developed a novel strategy to generate all-female (AF) common carp, which exhibited growth advantage over the control using genetic through single gene-targeting manipulation. Here, found that body weight of AF carp was higher by 22.58% than carp. Because genotype cyp17a1+/-;XX, contents sex steroids were normally synthesized, as comparable female To evaluate food safety Wistar rats fed diet containing (control, C) or at an incorporation rate 5, 10 20% (w/w) for 90 days. Compared with those no significant difference weight, intake, feed conversion ratio, hematology, serum biochemistry, urine test, relative organ gross necropsy, histopathological examination. This is first assessment farmed fish strain cultured CRISPR/Cas9, will further advance fishery development genome-edited animals.

Язык: Английский

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Generation of eco-friendly channel catfish,Ictalurus punctatus, harboring alligator cathelicidin gene with robust disease resistance by harnessing different CRISPR/Cas9-mediated systems

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Янв. 5, 2023

Abstract The CRISPR/Cas9 platform holds promise for modifying fish traits of interest as a precise and versatile tool genome manipulation. To reduce introgression transgene control reproduction, catfish species have been studied upscaled disease resistance intervening reproduction to lower the potential environmental risks escapees’ transgenic animals. Taking advantage CRISPR/Cas9-mediated system, we succeeded in integrating cathelicidin gene from an alligator ( Alligator sinensis ; As-Cath ) into target luteinizing hormone LH locus channel Ictalurus punctatus using two delivery systems assisted by double-stranded DNA (dsDNA) single-stranded oligodeoxynucleotides (ssODNs), respectively. In this study, high knock-in (KI) efficiency (22.38%, 64/286) but low on-target was achieved ssODN strategy, whereas adopting dsDNA donor template led efficient KI (10.80%, 23/213). On-target instrumental establishing knockout (LH − _As-Cath + line, which displayed heightened reduced fecundity compared wild-type sibling fish. Furthermore, implanting with HCG LHRHa can restore fecundity, spawnability hatchability new line. Overall, replaced then administered therapy gain complete reproductive disease-resistant environmentally sound manner. This strategy not only effectively improves consumer-valued traits, also guards against genetic contamination. is breakthrough aquaculture genetics confine prevent establishment or domestic genotypes natural environment.

Язык: Английский

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A social and ethical game-changer? An empirical ethics study of CRISPR in the salmon farming industry

Environmental Values, Год журнала: 2023, Номер 33(5), С. 476 - 494

Опубликована: Дек. 8, 2023

The genome editing technology CRISPR is described as a technological game-changer because of its flexibility and precision, an ethical due to ability engineer traits in living organisms without crossing species, avoiding significant objection genetically modified (GMOs). In salmon farming, applications breeding hold the promise handling environmental fish welfare challenges yet require social acceptance. Adopting empirical bioethics framework, this stakeholder interview study shows that respecting species borders important, but not decisive, for acceptance among Norwegian stakeholders. main objections are based on moral reflections about use outcomes. These combine principles pragmatic deliberations costs benefits, suggesting with environmentally ethically benefits can be socially acceptable. This indicates game-changing potential relies characteristics context which application takes place.

Язык: Английский

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Salma ved reisens slutt

Nytt Norsk Tidsskrift, Год журнала: 2024, Номер 41(3-4), С. 269 - 281

Опубликована: Дек. 11, 2024

Oppdrettsnæringen preges av store miljø- og velferdsutfordringer. Vi diskuterer en mulig løsning, nemlig genredigering oppdrettslaks ved bruk CRISPR. Pågående debatter om CRISPR handler hvorvidt slike teknologier skal klassifiseres som avl eller genmodifisering, hvordan de reguleres. argumenterer for at denne diskusjonen avsporer fra spørsmålet vi behandler laksen, må være konsistent med respektfull behandling laks, forstått våre medskapninger.

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