Fish & Shellfish Immunology,
Год журнала:
2023,
Номер
137, С. 108773 - 108773
Опубликована: Апрель 25, 2023
Misuse
and
overuse
of
antibiotics
in
aquaculture
has
proven
to
be
an
unsustainable
practice
leading
increased
bacterial
resistance.
An
alternative
strategy
involves
the
inclusion
immunostimulants
fish
diets,
especially
fungal
herbal
compounds
already
authorized
for
human
consumption,
hence
without
environmental
or
public
health
concerns.
In
this
study,
we
used
a
holistic
cross-disciplinary
pipeline
assess
immunostimulatory
properties
two
fungi:
Trametes
versicolor
Ganoderma
lucidum;
one
supplement,
capsaicin
form
Espelette
pepper
(Capsicum
annuum),
combination
these
additives
on
rainbow
trout
(Oncorhynchus
mykiss).
We
investigated
impact
diet
supplementation
7
weeks
survival,
growth
performance,
cellular,
humoral,
molecular
immune
parameters,
as
well
intestinal
microbial
composition
fish.
Uptake
influenced
expression
related
genes,
generating
inflammatory
response.
Significant
differences
were
detected
spleen-tlr2
gene
expression.
Supplementation
with
correlated
structural
changes
microbiota
enhanced
overall
diversity.
Results
demonstrated
that
different
treatments
had
no
adverse
effect
performance
suggesting
safety
feed
at
tested
concentrations.
While
mechanisms
multifactorial
interactions
remain
unclear,
study
provides
insights
not
only
regard
nutrition
compounds,
but
also
how
combined
gut
approach
can
shed
light
efficacy
immunostimulant
potential
commercial
supplements.
Journal of Fish Diseases,
Год журнала:
2024,
Номер
47(9)
Опубликована: Июнь 24, 2024
Abstract
Fish
meal
(FM)
replacement
is
essential
for
the
sustainable
expansion
of
aquaculture.
This
study
focussed
on
feasibility
replacing
FM
with
a
single‐cell
protein
(SCP)
derived
from
methanotrophic
bacteria
(
Methylococcus
capsulatus
,
Bath)
in
barramundi
fry
Lates
calcarifer
).
Three
isonitrogenous
and
isoenergetic
diets
were
formulated
0%,
6.4%
12.9%
inclusion
SCP,
by
25%
50%.
Barramundi
(initial
body
weight
2.5
±
0.1
g)
fed
experimental
21
days
to
assess
growth
performance,
gut
microbiome
composition
histopathology.
Our
findings
revealed
that
both
levels
SCP
induced
detrimental
effects
fry,
including
impaired
reduced
survival
compared
control
group
(66.7%
71.7%
50%,
respectively;
p
<
.05).
Both
dietary
treatments
presented
mild
necrotizing
enteritis
subepithelial
oedema
accumulation
PAS
positive,
diastase
resistant
droplets
within
hepatocytes
(ceroid
hepatopathy)
pancreatic
atrophy.
Microbiome
analysis
marked
shift
microbial
community
potential
opportunistic
genus
Aeromonas
.
Reduced
overall
performance
highest
level
50%
)
was
primarily
associated
feed
intake,
likely
related
palatability
issues,
albeit
pathological
changes
observed
liver
may
also
play
role.
highlights
importance
meticulous
optimization
aquafeed
formulations,
need
species
life‐stage
specific
assessments
ensure
health
welfare
fish
aquaculture
practices.
