bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 24, 2024
Atlantic
salmon
(
Salmo
salar
)
when
exposed
to
heat
stress
have
reduced
voluntary
feeding
and
exhibit
changes
in
digesta
consistency.
This
study
was
performed
determine
what
bacterial
species
occur
the
overall
abundance
gut
microbiome
stressed
salmon.
For
this
seawater
tanks
at
15°C
were
fed
for
2
4
weeks.
Tank
temperatures
increased
19°C
until
abated
which
point
tank
cooled
At
end
of
each
temperature
phase
fish
stripped
feces
profiles
determined
using
16S
rRNA
V1-V3
metabarcoding.
The
experiment
repeated
three
times
successive
years.
Vibrionaceae
comprised
most
reads
after
warm
completed.
prominent
levels
accompanied
by
a
large
predominance
cast
(sloughed
intestinal
mucosa)
containing
fecal
samples.
qPCR
estimated
cell
populations
1.9-3.4
log
units/g
but
slightly
decreased
0.3-1.1
recovery
phase.
results
indicated
induced
inappetence
corresponds
production
Vibrionaceae.
colonizing
should
be
focus
studies
on
microbiology
thermally
dysbiosis
Aquaculture Reports,
Год журнала:
2024,
Номер
37, С. 102211 - 102211
Опубликована: Июль 5, 2024
A
5-level
crowding
intensity
scale
for
directing
and
auditing
the
of
Atlantic
salmon
in
sea
cages
based
on
surface
observations
is
currently
included
standards,
manuals,
guidelines
fish
farmers.
Here
we
test
feasibility
using
this
to
create
distinct
levels,
effects
these
different
levels
upon
welfare,
suitability
a
set
possible
operational
welfare
indicators
(OWIs)
laboratory-based
(LABWIs)
be
toolboxes
monitoring
assessing
relation
cages.
Crowding
level
1
was
not
study
since
very
light
crowding,
also
5
as
clearly
would
harm
lead
mortalities.
We
were
able
use
three
two
separate
events
12×12m2
Although
farm
personnel
experienced,
it
soon
became
evident
that
underwater
behaviour
how
net
tightened
around
important
make
sure
no
pockets
or
irregularities
could
formed
during
crowding.
Despite
evidence
increased
stress
epidermal
damage
with
intensity,
there
clear
indications
led
any
long-term
detrimental
welfare.
In
conclusion
an
OWI-toolbox
should
include
both
observations,
oxygen
conditions,
morphological
injury
data
steer
decisions
prevent
problems
addition,
qualitative
assessment
behaviour,
plasma
cortisol,
skin
histology
can
LABWI-toolbox
if
more
in-depth
information
from
wanted.
Machine Learning with Applications,
Год журнала:
2024,
Номер
16, С. 100562 - 100562
Опубликована: Май 18, 2024
During
the
production
of
salmonids
in
aquaculture,
it
is
common
to
observe
growth-stunted
individuals.
The
cause
for
so-called
"loser
fish
syndrome"
unclear,
which
needs
further
investigation.
Here,
we
present
and
compare
computer
vision
systems
automatic
detection
classification
loser
Atlantic
salmon
images
taken
sea
cages.
We
evaluated
two
end-to-end
approaches
(combined
classification)
based
on
YoloV5
YoloV7,
a
two-stage
approach
transfer
learning
an
ensemble
classifiers
(e.g.,
linear
perception,
Adaline,
C-support
vector,
K-nearest
neighbours,
multi-layer
perceptron)
classification.
To
our
knowledge,
use
classifiers,
considering
consolidated
proposed
literature,
has
not
been
applied
this
problem
before.
Classification
entailed
assigning
every
healthy
class.
results
were
compared
reliability
human
best-performing
was
reached
precision
score
86.30
%,
recall
71.75
F1
78.35
%.
presented
79.7
while
66.05
Human
had
substantial
agreement
strength
(Fleiss'
Kappa
0.68),
highlighting
that
evaluation
by
subjective.
Our
system
will
enable
farmers
researchers
follow
abundance
losers
throughout
period.
provide
dataset
annotated
research.
Reviews in Aquaculture,
Год журнала:
2024,
Номер
17(1)
Опубликована: Дек. 9, 2024
ABSTRACT
As
Atlantic
salmon
depend
on
dissolved
oxygen
(DO)
to
survive
and
grow,
managing
DO
is
essential
for
welfare
growth.
However,
the
dynamics
of
in
open
sea
cages
are
complex,
caused
by
a
range
factors
that
interact
create
compounding
effects.
To
understand
dynamics,
two
pillars
knowledge
essential:
supply
consumption
cages.
The
components
these
vary
greatly
across
both
time
space,
with
including
ambient
temperature
current,
density
stratification,
fish
size,
health
metabolism,
biomass
distribution,
topography,
production
routines
farm
configuration.
In
environment,
often
interact,
making
related
analyses
effects,
causality
effective
management
highly
challenging.
Exacerbating
matters,
existing
fragmented
multiple
disciplines,
it
challenging
end‐users
access.
Using
sensors
necessary
manage
complexity
DO,
interest
implement
digital
technologies
aquaculture
operations
utilize
big
data
growing,
but
succeed
application,
an
in‐depth
understanding
relevant
necessary.
Consequently,
this
review
produced
compile
many
influence
or
cages,
their
relationship
each
other.
narrative
review,
we
explore
known
interactions
effects
between
factors,
synthesize
causal
diagram
holistic
balance.
Environmental Microbiology,
Год журнала:
2024,
Номер
26(10)
Опубликована: Окт. 1, 2024
Abstract
Geosmin
and
2‐methylisoborneol
(MIB)
are
known
to
cause
taste‐and‐odour
problems
in
recirculating
aquaculture
systems
(RAS).
Both
geosmin
MIB
microbial
metabolites
belonging
terpenoids.
Precursors
for
terpenoids
biosynthesized
via
the
methylerythritol
phosphate
(MEP)
mevalonate
(MVA)
pathways.
We
carried
out
a
metagenomic
analysis
of
50
samples
from
five
RAS
investigate
terpenoid
biosynthesis
metabolic
potential
production
microbiomes.
A
total
1008
metagenome‐assembled
genomes
(MAGs)
representing
26
bacterial
three
archaeal
phyla
were
recovered.
Although
most
archaea
thought
use
MVA
pathway
precursor
biosynthesis,
an
Iainarchaeota
MAG
is
shown
harbour
complete
set
genes
encoding
MEP
but
lacking
associated
with
pathway.
In
this
study,
16
MAGs
affiliated
(
Acidobacteriota
,
Actinobacteriota
Bacteroidota
Chloroflexota
Myxococcota
)
identified
as
possessing
or
synthases.
These
putative
taste
odour
producers
diverse,
many
taxonomically
unidentified
at
genus
species
level,
their
relative
abundance
differed
between
investigated
farms.
The
study
microbiomes
revealed
previously
unknown
phylogenetic
diversity
produce
MIB.
