Biomonitoring of Waters and Tambacu (Colossoma macropomum × Piaractus mesopotamicus) from the Amazônia Legal, Brazil

Water, Год журнала: 2024, Номер 16(18), С. 2588 - 2588

Опубликована: Сен. 12, 2024

Fish farming is increasingly important globally and nationally, playing a crucial role in fish production for human consumption. Monitoring microbiological chemical contaminants from water discharge essential to mitigate the risk of contaminating This study analyzes physicochemical E. coli parameters tambacu muscles (Colossoma macropomum × Piaractus mesopotamicus) Western Maranhão, Brazil. It also includes qualitative characterization zooplankton ponds. Samples were collected ponds dam system fed by natural watercourses Tocantins River tributaries, located at connection Brazilian savanna Amazon biomes. The did not meet national standards. community included Rotifera, Cladocera, Copepoda, Protozoa representatives, with no prior studies on region, making these findings unprecedented. biological quality freshwater farming, as poor can lead decreased productivity mortality, raising significant food safety concerns. studied related potential influence untreated wastewater source contamination, leaving region still far safe reuse practices. contamination waters concern health emphasize need appropriate regulations.

Язык: Английский

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Spatio-temporal fish gill microbiota analysis as indicators in estuarine fish health monitoring

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 6, 2024

Coastal marine and estuarine systems are subject to enormous endogenous exogenous pressures, particularly climate change, while at the same time being highly productive sources nurseries for fish populations. Interactions between host microbiome increasingly recognized their importance health, with growing evidence indicating that abundance virulence of pathogenic bacteria rapidly increasing. The microbial composition gill mucus reflects environmental conditions represents a major entry route pathogens into body. High-throughput sequencing prokaryotic populations from 250 samples two species different habitat preferences, as well seasonal spatial distributions in Elbe estuary system, allowed us describe variation microbiota along salinity gradient under fluctuating conditions. analysis core relation variable bacterial components indicated dysbiotic states sustained hypoxia high nutrient loads largely driven by takeover opportunistic (Acinetobacter, Shewanella, Aeromonas). By correlating abundances physiological parameters co-occurrence network approach, we plasticity composition, identify biomarkers health reconstruct movement patterns fish. Our results will help shape future non-invasive cost-effective monitoring programs, factors might be controlled promote stock health.

Язык: Английский

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Spatio-temporal plasticity of gill microbiota in estuarine fish

The Science of The Total Environment, Год журнала: 2024, Номер 957, С. 177505 - 177505

Опубликована: Ноя. 17, 2024

Coastal marine and estuarine systems are subject to enormous endogenous exogenous pressures, particularly climate change, while at the same time being highly productive sources nurseries for fish populations. Interactions between host microbiome increasingly recognized their importance health, with growing evidence indicating that increasing environmental pressures impact resilience favor raise of opportunistic bacterial taxa. The microbial composition gill mucus reflects conditions represents an entry route pathogens into body. High-throughput sequencing prokaryotic populations from 250 samples two species different habitat preferences, as well seasonal spatial distributions in Elbe estuary system, allowed us describe variation microbiota along a salinity gradient under fluctuating conditions. analysis core relation variable components indicated dysbiotic states sustained hypoxia high nutrient loads largely driven by increased prevalence facultatively aerobic (Acinetobacter) anaerobic heterotrophs (Shewanella, Aeromonas). By correlating abundances physiological parameters co-occurrence network approach, we plasticity composition, identify potential biomarkers health monitoring reconstruct movement patterns fish. Our results can help shape future minimal-invasive cost-effective programs, factors need be controlled promote stock health.

Язык: Английский

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Unveiling Hidden Allies: In Silico Discovery of Prophages in Tenacibaculum Species

Antibiotics, Год журнала: 2024, Номер 13(12), С. 1184 - 1184

Опубликована: Дек. 5, 2024

Tenacibaculosis, caused by Tenacibaculum species, is a significant disease in aquaculture, leading to high mortality and economic losses. Antibiotic treatment raises concerns about resistance, making phage therapy an interesting alternative. Analyzing traces genomes crucial for developing these bacteriophage-based strategies. Methods: We assessed the presence of prophages 212 genomes/assemblies available NCBI repository, comprising several species global locations, using PHASTEST program. Then, we focused on those regions classified as intact, evaluating most common phages found VICTOR. The protein interest discovered was evaluated ProtParam, DeepTMHMM, InterPro, Phyre2 tools. In addition, antiphage defense systems with intact DefenseFinder tool. Results: identified 25 elements 24 out analyzed, 11% assemblies containing elements. These were concentrated T. maritimum mesophilum, which harbored 10 7 prophage regions, respectively. Of elements, six including four maritimum, belonging Pippivirus Siphoviridae families. Bioinformatic analysis showed that putative endolysin stable 432 amino acids 49.8 kDa, three transmembrane helices CHAP domain, structurally similar lytic domain S. aureus bacteriophage K. Conclusions: Key Tenacibaculum, especially show promise against tenacibaculosis, supporting sustainable, antibiotic-free treatments aquaculture.

Язык: Английский

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Optimising Outbreak Investigation, Surveillance and Discovery of Pathogens in Aquaculture Using Unbiased Metatranscriptomics

Reviews in Aquaculture, Год журнала: 2024, Номер 17(1)

Опубликована: Дек. 19, 2024

ABSTRACT Emerging infectious diseases are a major threat to global aquaculture production and projected increase in frequency due anthropogenic stressors climate change. Our ability screen the aquatic ‘infectome’ is dramatically improving through unbiased metatranscriptomic (i.e., total RNA) sequencing. Despite recent advances molecular diagnostics next‐generation sequencing, outbreak investigation often encounters limitations targeted nature of conventional techniques, which may only capture small portion diverse array disease‐causing agents present nature, sometimes requiring years resolve. Here, we propose that sequencing serves as cost‐effective powerful technique for characterising pathogens during disease outbreaks surveillance programmes. In doing so, offer diagnostic framework mitigate threats aquaculture. We emphasise importance combining rapid metatranscriptomics with traditional techniques holistic examination causes unknown aetiologies animals, how it can be used examine host responses parallel pathogen discovery. suggest integration into will require upskilling, proficiency testing, user‐friendly bioinformatic tools open data sharing. Furthermore, essential establish reporting framework—with analytical guidelines—that ensures protection animal industries while safeguarding trade interests.

Язык: Английский

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