What is the importance of sperm subpopulations?

Animal Reproduction Science, Год журнала: 2021, Номер 246, С. 106844 - 106844

Опубликована: Сен. 11, 2021

The study of sperm subpopulations spans three decades. origin, meaning, and practical significance, however, are less clear. Current technology for assessing morphology (CASA-Morph) motility (CASA-Mot) has enabled the accurate evaluation these features, there many options data classification. Subpopulations could occur as a result stage development each spermatozoon in subpopulation. Spermatogenesis might contribute to production subpopulations. Insights from evolutionary biology recent molecular research indicative diversity among male gametes that unequal sharing transcripts other elements through cytoplasmic bridges between spermatids. Sperm cohorts exiting gonads would contain different RNA protein contents, affecting physiology associations with surrounding environmental milieu. Subsequently, differences affect how spermatozoa interact milieu (maturation, mixing seminal plasma, interacting milieu, or female genital tract gamete). emergence an outcome evolution, related reproductive strategies species, structures, copulatory fertilization processes. This kind approach determining importance capacity should have impact conducting technologies, inspiring enabling new ways more efficient use medical, animal breeding, conservation fields. manuscript is contribution Special Issue memory Dr. Duane Garner.

Язык: Английский

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CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement

Current Biology, Год журнала: 2023, Номер 33(16), С. 3371 - 3388.e7

Опубликована: Июль 25, 2023

The molecular mechanism underlying asymmetric axonemal complexes in sperm flagella is still largely unknown. Here, we showed that the knockout of coiled-coil domain-containing 176 (CCDC176) mice led to male infertility due decreased motility. Ccdc176 specifically destabilized microtubule doublets (MTDs) 1 and 9 during maturation corpus epididymis. Single-sperm immunofluorescence most CCDC176 was distributed along axoneme, further super-resolution imaging revealed asymmetrically localized axoneme. could cooperate with radial spoke proteins stabilize MTDs 9, its results destabilization some flagella. Furthermore, as predicted by multibody dynamics (MBD) model, found jutted out from flagellum annulus region

Язык: Английский

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Atypical Centriolar Composition Correlates with Internal Fertilization in Fish

Cells, Год журнала: 2022, Номер 11(5), С. 758 - 758

Опубликована: Фев. 22, 2022

The sperm competition theory, as proposed by Geoff Parker, predicts that evolve through a cascade of changes. As an example, internal fertilization is followed morphology diversification. However, little known about the evolution structures. centriole has ancient and evolutionarily conserved canonical structure with signature 9-fold, radially symmetric microtubules form cell’s centrosomes, cilia, flagella. Most animal spermatozoa have two centrioles, one which forms spermatozoan flagellum. Both are delivered to egg constitute embryo’s first centrosomes. mammals insects only recognizable structure. A second atypical was recently reported in both groups which, prior this, eluded discovery standard techniques criteria. Because ancestors reproduced fertilization, we hypothesized transition from centrioles composition ancestral centriolar characterized evolved preferentially after fertilization. We examined fish because diversity species available test this hypothesis–as some reproduce via others external fertilization–and their ultrastructure been extensively studied. Our literature search reports on 277 species. Species specifically enriched among fertilizers compared (7/34, 20.6% versus 2/243, 0.80%; p < 0.00001, odds ratio = 32.4) represent phylogenetically unrelated fish. Atypical present subfamily Poeciliinae. Therefore, internally fertilizing independently multiple times, agreeing Parker’s theory.

Язык: Английский

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Sexual selection drives the coevolution of male and female reproductive traits in Peromyscus mice

Journal of Evolutionary Biology, Год журнала: 2022, Номер 36(1), С. 67 - 81

Опубликована: Дек. 8, 2022

Abstract When females mate with multiple partners within a single reproductive cycle, sperm from rival males may compete for fertilization of limited number ova, and bias the their ova by particular sperm. Over evolutionary timescales, these two forms selection shape both male female physiology when multiply, yet in monogamous systems, post‐copulatory sexual is weak or absent. Here, we examine how divergent mating strategies genus closely related mice, Peromyscus , have shaped evolution traits. We show that promiscuous species, exhibit traits associated increased production swimming performance, are predicted to limit access including oviduct length larger cumulus cell mass surrounding compared species. Importantly, found across density significantly correlated velocity, but not count relative testes size, suggesting coevolved quality rather than quantity. Taken together, our results highlight evolve concert respond changes level selection.

Язык: Английский

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New Insights Into Sperm Ultrastructure Through Enhanced Scanning Electron Microscopy

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9

Опубликована: Апрель 22, 2021

The analysis of spermatozoa morphology is fundamental to understand male fertility and the etiology infertility. Traditionally scanning electron microscopy (SEM) has been used define surface topology. Recently, however, it become a critical tool for three-dimensional internal cellular ultrastructure. Modern SEM provides nanometer-scale resolution, but meaningfulness such information proportional quality sample preservation. In this study, we demonstrate that sperm quickly robustly adhere gold-coated surfaces. Leveraging property, developed three step-by-step protocols fulfilling different needs imaging: chemically fixed monolayers examination external morphology, two high-pressure freezing-based fast full cell focused ion-beam tomography. These analyses allow previously unappreciated insights into mouse ultrastructure, including identification novel structures within fibrous sheath domain-specific interactions between plasma membrane exosome-like structures.

Язык: Английский

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CCDC113 stabilizes sperm axoneme and head-tail coupling apparatus to ensure male fertility

eLife, Год журнала: 2024, Номер 13

Опубликована: Июнь 5, 2024

The structural integrity of the sperm is crucial for male fertility, defects in head-tail linkage and flagellar axoneme are associated with acephalic spermatozoa syndrome (ASS) multiple morphological abnormalities flagella (MMAF). Notably, impaired coupling apparatus (HTCA) often accompanies flagellum structure, however, molecular mechanisms underlying this phenomenon remain elusive. Here, we identified an evolutionarily conserved coiled-coil domain-containing (CCDC) protein, CCDC113, found disruption CCDC113 produced disorganized HTCA, which caused infertility. Further analysis revealed that could bind to CFAP57 CFAP91, function as adaptor protein connection radial spokes, nexin-dynein regulatory complex (N-DRC), doublet microtubules (DMTs) axoneme. Moreover, was a component collaborating SUN5 CENTLEIN connect head tail during spermiogenesis. Together, our studies reveal serve critical hub HTCA stabilization mice, providing insights into potential pathogenesis infertility human mutations.

Язык: Английский

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Effect of Chemical Pollution on the Fertility of Male Rodents from Natural Populations: Сomparing the Response of Sperm Morphology, Motility, and Concentration

Russian Journal of Ecology, Год журнала: 2024, Номер 55(3), С. 201 - 217

Опубликована: Июнь 1, 2024

Язык: Английский

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Microenvironment of the male and female reproductive tracts regulate sperm fertility: Impact of viscosity, pH, and osmolality

Andrology, Год журнала: 2021, Номер 10(1), С. 92 - 104

Опубликована: Авг. 22, 2021

Terminally differentiated mammalian sperm are exposed to gradients of viscosity, pH, and osmolality both in the male female reproductive tract during their perilous journey quest ovum. The complex physicochemical factors play an integral role preparing for fertilization process.To elucidate influence microenvironment especially regulating functional competence.The data used this review were collected from research papers online databases focusing on function.The exist across various segments tract. changes viscosity create a physical barrier, pH aid capacitation hyperactivation, osmotic stress selects progressive subpopulation accomplishing fertilization. function tests developed based concept that genotype is major contributor outcome. However, recent studies demonstrate significance genotype-environment interactions essentially contributing success. Hence, it imperative assess impact stresses adaptive ability terminally sperm, which turn would improve outcome assisted technologies fertility assessment.Elucidating provides newer insights into procedures need be adopted selecting fertile males breeding, ejaculates technologies.

Язык: Английский

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Differences in sperm morphology between Alouatta palliata and Alouatta pigra are consistent with the intensity of sperm competition in each species

American Journal of Primatology, Год журнала: 2023, Номер 85(10)

Опубликована: Июль 24, 2023

The intensity of sperm competition, in which compete within the female reproductive tract to reach and fertilize her eggs, varies species with different mating systems. Sperm competition is more intense where males cannot monopolize access females mate multiple males. In this scenario, a morphological change that increases ability eggs should rapidly spread population, leading differences between closely related species. Differences morphology have been reported among primate However, due inherent logistical ethical difficulties sample from wild primates, extent variation remains understudied. Here, we compared traits two sister howler monkey (Alouatta palliata Alouatta pigra) systems investigate effect on traits. We predicted A. palliata, opportunities males, would show associated increase competitiveness pigra mostly central male. used linear mixed models determine morphology, controlling for individual variation. found midpieces heads were average 26.2% 11.0% longer, respectively, than those pigra. these are important speed hydrodynamic movement other can affect fertilization success. This study provides empirical evidence evolved through sexual selection

Язык: Английский

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Effect of High Viscosity on Energy Metabolism and Kinematics of Spermatozoa from Three Mouse Species Incubated under Capacitating Conditions

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(23), С. 15247 - 15247

Опубликована: Дек. 3, 2022

In order to sustain motility and prepare for fertilization, sperm require energy. The characterization of ATP production usage in mouse species revealed substantial differences metabolic pathways that can be differentially affected by capacitation. Moreover, spermatozoa encounter different environments with varying viscoelastic properties the female reproductive tract. Here, we examine whether viscosity affects levels kinematics during capacitation vitro. Sperm from three (Mus musculus, M. spretus, spicilegus) were incubated under capacitating conditions a modified Tyrode's medium containing bicarbonate, glucose, pyruvate, lactate, bovine serum albumin (mT-BH) or bicarbonate-free as non-capacitating control. Viscosity was increased inclusion polyvinylpyrrolidone. measured bioluminescence kit, examined computer-aided analysis system. musculus sperm, declined capacitation, but no found between sperm. contrast, spretus spicilegus, decreased Increasing did not modify timing proportion cells undergoing result additional time- concentration-dependent decreases spicilegus conditions. Additionally, altered both velocity trajectory descriptors. limited impact higher on could related low intensity postcopulatory sexual selection this species. Responses seen other two linked ability their perform better enhanced selective pressures.

Язык: Английский

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