Qilongtian Ameliorate Bleomycin-induced Pulmonary Fibrosis in Mice via Inhibiting IL-17 signal pathway DOI Creative Commons
Qiang Zhang, Ting Luo,

Dezheng Yuan

и другие.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Ноя. 21, 2022

Abstract Pulmonary fibrosis (PF) is a special type of pulmonary parenchymal disease, with chronic, progressive, fibrosis, high mortality. There lack safe, effective, and affordable treatment methods. Qilongtian (QLT) traditional Chinese prescription that composed Panax notoginseng, Earthworm, Rhodiola, shows the remarkable clinical curative effect PF. However, mechanism QLT remains to be clarified. Therefore, we studied effectivity in treating Bleomycin (BLM) induced PF mice. Our studies have shown significantly reduced inflammatory injury, hydroxyproline content collagen deposition lung tissue BLM mice downregulated cytokine related inflammation expression on mRNA protein level To identify QLT, Transcriptome was measured, IL-17 signal pathway screened out for further research. Further indicated CCL12, CXCL5, FOSL1, MMP9 , AREG which are fibrosis-related genes pathway. In summary, results potential preventing progress by inhibiting resulting study provides novel scientific basis represents new therapeutics clinical.

Язык: Английский

IL-39 increases ROS production and promotes the phosphorylation of p38 MAPK in the apoptotic cardiomyocytes DOI Creative Commons
Wei Xiong, Hua Chen,

Jiequan Lu

и другие.

Folia Histochemica et Cytobiologica, Год журнала: 2021, Номер 59(3), С. 195 - 202

Опубликована: Сен. 20, 2021

Introduction. The cytokine interleukin (IL)-39 is a novel member of the IL-12 family. Our previous study found that serum level IL-39 significantly increased in patients with acute myocardial infarction. However, role cardiomyocyte apoptosis remains unclear. Material and methods. In this study, cultured mouse HL-1 cardiomyocytes were incubated PBS, 0-100 ng/mL IL-39, 200 μM H2O2 or 20 Trolox. Results. promoted production intracellular reactive oxygen species (ROS) concentration dependent manner cardiomyocytes. both ROS, CCL2, stimulated apoptotic progress cardiomyocytes, mRNA protein expression levels Bax, caspase-3, p-p38 MAPK, decreased Bcl-2. ROS production, CCL2 level, apoptosis, MAPK amplified by administration combined H2O2, these processes alleviated an antioxidant Conclusion. This was revealing stimulating phosphorylation p38

Язык: Английский

Процитировано

5
IL-17 in skin infections and homeostasis DOI Creative Commons

María Guadalupe García-Patiño,

Cleofas Marcial-Medina, Blanca E. Ruiz‐Medina

и другие.

Clinical Immunology, Год журнала: 2024, Номер 267, С. 110352 - 110352

Опубликована: Авг. 30, 2024

Interleukin (IL) 17 is a proinflammatory cytokine belonging to structurally related group of cytokines known as the IL-17 family. It has been profoundly studied for its contribution pathology autoimmune diseases. However, it also plays an important role in homeostasis and defense against extracellular bacteria fungi. epithelial barriers, including skin, where some cellular targets reside. Most research work on focused effects skin within context diseases or sterile inflammation, despite having impact other conditions. In recent years, studies pathogens maintenance mediated by microbiota have grown importance. Here we review discuss cumulative evidence regarding main integrity well protective pathogenic during infections.

Язык: Английский

Процитировано

0
Pasteurella multocida Serotype D Infection Induces Activation of the IL-17 Signaling Pathway in Goat Lymphocytes DOI Creative Commons

Yujing Fu,

Yong Meng,

Hejie Qian

и другие.

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2618 - 2618

Опубликована: Дек. 18, 2024

(1) Background: Pasteurellosis is a global zoonotic bacterial disease, which has caused significant economic impacts in animal husbandry. Nevertheless, there limited understanding of the immune response between goat peripheral blood lymphocytes (PBLs) and goat-derived Pasteurella multocida (P. multocida). (2) Methods: To investigate host PBLs during infection with P. type D, we established an vitro cell model utilizing isolated primary PBLs. Utilizing this model, employed enzyme-linked immunosorbent assay (ELISA) to assess cytokine profile variation following infection. Meanwhile, RNA sequencing quantitative PCR (qPCR) methods were analyze gene expression profile. (3) Results: The ELISA test results indicated that levels pro-inflammatory cytokines, such as IL-6, IFN-γ, CXCL10, IL-17A, significantly elevated within 12 h after multocida. In contrast, anti-inflammatory IL-10 found be reduced. functional enrichment analysis identified 2114 differentially expressed genes (DEGs) primarily associated cytokine-cytokine receptor interactions, viral protein-cytokine IL-17 signaling pathway. Furthermore, protein-protein interaction (PPI) network qPCR highlighted CD86, CCL5, CD8A, CXCL8, CTLA4, TNF, CD274, IL-10, IFNG, IL-17A crucial for (4) Conclusions: This study systematically revealed characteristics goats D through cytokines expression, providing important theoretical insights deeper defense mechanisms against

Язык: Английский

Процитировано

0
Identification of genes associated with sudden cardiac death: a network- and pathway-based approach DOI Open Access
Jinhuan Wei, Xuejun Ni, Yanfei Dai

и другие.

Journal of Thoracic Disease, Год журнала: 2021, Номер 13(6), С. 3610 - 3627

Опубликована: Июнь 1, 2021

Sudden cardiac death (SCD) accounts for a large proportion of the total deaths across different age groups. Although numerous candidate genes related to SCD have been identified by genetic association studies and genome wide (GWAS), molecular mechanisms underlying are still unclear, biological functions interactions these remain obscure. To clarify this issue, we performed comprehensive systematic analysis SCD-related network pathway-based approach.By screening publications deposited in PubMed Gene-Cloud Biotechnology Information (GCBI) databases, collected genetically associated with SCD, which were referred as gene set (SCDgset). analyze processes biochemical pathways genes, functional was performed. explore interlinks enriched pathways, pathway crosstalk implemented. construct SCD-specific networks, Markov cluster algorithm Steiner minimal tree employed.We 257 that reported be summarized them SCDgset. Most heart diseases, while some may noncardiac causes SCD. The could roughly grouped into two modules. One module calcium signaling other MAPK pathway. Moreover, networks inferred, 23 novel potentially also identified.In summary, means methodology, explored pathogenetic mechanism Our results provide valuable information understanding pathogenesis include biomarkers diagnosing potential patients diseases; help reducing corresponding risks even aid preventing

Язык: Английский

Процитировано

1
Qilongtian Ameliorate Bleomycin-induced Pulmonary Fibrosis in Mice via Inhibiting IL-17 signal pathway DOI Creative Commons
Qiang Zhang, Ting Luo,

Dezheng Yuan

и другие.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Ноя. 21, 2022

Abstract Pulmonary fibrosis (PF) is a special type of pulmonary parenchymal disease, with chronic, progressive, fibrosis, high mortality. There lack safe, effective, and affordable treatment methods. Qilongtian (QLT) traditional Chinese prescription that composed Panax notoginseng, Earthworm, Rhodiola, shows the remarkable clinical curative effect PF. However, mechanism QLT remains to be clarified. Therefore, we studied effectivity in treating Bleomycin (BLM) induced PF mice. Our studies have shown significantly reduced inflammatory injury, hydroxyproline content collagen deposition lung tissue BLM mice downregulated cytokine related inflammation expression on mRNA protein level To identify QLT, Transcriptome was measured, IL-17 signal pathway screened out for further research. Further indicated CCL12, CXCL5, FOSL1, MMP9 , AREG which are fibrosis-related genes pathway. In summary, results potential preventing progress by inhibiting resulting study provides novel scientific basis represents new therapeutics clinical.

Язык: Английский

Процитировано

0