ABSTRACT
A
reduced
rate
of
stem
cell
division
is
considered
a
widespread
feature
which
ensures
the
integrity
genetic
information
during
somatic
development
plants
and
animals.
Radial
growth
plant
shoots
roots
cell-driven
process
that
fundamental
for
mechanical
physiological
support
enlarging
bodies.
In
most
dicotyledonous
species,
underlying
niche,
cambium,
generates
xylem
inwards
phloem
outwards.
Despite
importance
intriguing
dynamics
functional
characterization
its
cells
hampered
by
lack
experimental
tools
accessing
distinct
cambium
sub-domains.
Here,
we
use
hypocotyl
Arabidopsis
thaliana
to
map
activity
in
proliferating
cambium.
Through
pulse
labeling
genetically
encoded
lineage
tracing,
find
single
bifacial
both
lineages.
This
characterized
specific
combination
PXY
(TDR),
SMXL5
WOX4
gene
high
comparison
with
tissue-specific
progenitors.
Our
analysis
provides
cellular
fate
radial
growth,
suggests
quiescence
not
general
prerequisite
life-long
tissue
production.
article
has
an
associated
‘The
people
behind
papers’
interview.
New Phytologist,
Год журнала:
2022,
Номер
235(3), С. 848 - 866
Опубликована: Май 5, 2022
Summary
Outer
protective
barriers
of
animals
use
a
variety
bio‐polymers,
based
on
either
proteins
(e.g.
collagens),
or
modified
sugars
chitin).
Plants,
however,
have
come
up
with
particular
solution,
the
polymerisation
lipid‐like
precursors,
giving
rise
to
cutin
and
suberin.
Suberin
is
structural
lipophilic
polyester
fatty
acids,
glycerol
some
aromatics
found
in
cell
walls
phellem,
endodermis,
exodermis,
wound
tissues,
abscission
zones,
bundle
sheath
other
tissues.
It
deposits
as
hydrophobic
layer
between
(ligno)cellulosic
primary
wall
plasma
membrane.
highly
against
biotic
abiotic
stresses,
shows
great
developmental
plasticity
its
chemically
recalcitrant
nature
might
assist
sequestration
atmospheric
carbon
by
plants.
The
aim
this
review
integrate
rapidly
accelerating
genetic
biological
discoveries
recent
years
important
chemical
contributions
obtained
from
very
diverse
organisms
tissue
layers.
We
critically
discuss
order
localisation
enzymatic
machinery
synthesising
presumed
substrates
for
export
apoplastic
polymerisation.
attempt
explain
observed
suberin
linkages
enzyme
activities
spatiotemporal
relationship
lignin
ferulates,
necessary
produce
functional
suberised
wall.
Science,
Год журнала:
2023,
Номер
382(6669), С. 464 - 471
Опубликована: Окт. 26, 2023
Functionally
similar
to
the
tight
junctions
present
in
animal
guts,
plant
roots
have
evolved
a
lignified
Casparian
strip
as
an
extracellular
diffusion
barrier
endodermis
seal
root
apoplast
and
maintain
nutrient
homeostasis.
How
this
is
structured
has
been
partially
defined,
but
its
lignin
polymerization
assembly
steps
remain
elusive.
Here,
we
characterize
family
of
dirigent
proteins
(DPs)
essential
for
both
localized
required
biogenesis
cell
wall
attachment
plasma
membrane
apoplast.
We
reveal
lignification
mechanism
that
requires
cooperation
between
DPs
Schengen
pathway.
Furthermore,
demonstrate
directly
mediate
part
mechanism.
Nature Plants,
Год журнала:
2023,
Номер
9(4), С. 631 - 644
Опубликована: Март 30, 2023
Abstract
Vascular
cambium
contains
bifacial
stem
cells,
which
produce
secondary
xylem
to
one
side
and
phloem
the
other.
However,
how
these
fate
decisions
are
regulated
is
unknown.
Here
we
show
that
positioning
of
an
auxin
signalling
maximum
within
determines
cell
daughters.
The
position
modulated
by
gibberellin-regulated,
PIN1-dependent
polar
transport.
Gibberellin
treatment
broadens
from
towards
phloem.
As
a
result,
xylem-side
daughter
preferentially
differentiates
into
xylem,
while
phloem-side
retains
identity.
Occasionally,
this
broadening
leads
direct
specification
both
daughters
as
consequently,
adjacent
phloem-identity
reverts
being
cell.
Conversely,
reduced
gibberellin
levels
favour
Together,
our
data
provide
mechanism
regulates
ratio
production.
Science,
Год журнала:
2025,
Номер
387(6734), С. 666 - 673
Опубликована: Фев. 6, 2025
Plants
grow
complex
root
systems
to
extract
unevenly
distributed
resources
from
soils.
Spatial
differences
in
soil
moisture
are
perceived
by
tips,
leading
the
patterning
of
new
branches
toward
available
water
a
process
called
hydropatterning.
Little
is
known
about
hydropatterning
behavior
and
its
genetic
basis
crop
plants.
Here,
we
developed
an
assay
measure
maize
revealed
substantial
between
tropical/subtropical
temperate
breeding
germplasm
that
likely
resulted
divergent
selection.
Genetic
analysis
confirmed
regulatory
role
auxin
gaseous
hormone
ethylene
locally
inhibits
branching
air-exposed
tissues.
Our
results
demonstrate
how
distinct
signaling
pathways
translate
spatial
patterns
availability
developmental
programs
determine
architecture.
Developmental Cell,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Reactivation
of
cell
division
is
crucial
for
the
regeneration
damaged
tissues,
which
a
fundamental
process
across
all
multicellular
organisms.
However,
mechanisms
underlying
activation
in
plants
during
remain
poorly
understood.
Here,
we
show
that
single-cell
endodermal
ablation
generates
transient
change
local
mechanical
pressure
on
neighboring
pericycle
cells
to
activate
patterned
tissue
Arabidopsis
roots.
Moreover,
provide
strong
evidence
this
relies
phytohormone
ethylene.
Thus,
our
results
highlight
previously
unrecognized
role
mechano-chemical
control
plants.
Science,
Год журнала:
2020,
Номер
367(6476), С. 431 - 435
Опубликована: Янв. 24, 2020
The
plant
embryonic
cuticle
is
a
hydrophobic
barrier
deposited
de
novo
by
the
embryo
during
seed
development.
At
germination,
it
protects
seedling
from
water
loss
and
is,
thus,
critical
for
survival.
Embryonic
formation
controlled
signaling
pathway
involving
ABNORMAL
LEAF
SHAPE1
subtilase
two
GASSHO
receptor-like
kinases.
We
show
that
sulfated
peptide,
TWISTED
SEED1
(TWS1),
acts
as
ligand.
Cuticle
surveillance
depends
on
action
of
subtilase,
which,
unlike
TWS1
precursor
receptors,
not
produced
in
but
neighboring
endosperm.
Subtilase-mediated
processing
embryo-derived
releases
active
triggering
GASSHO-dependent
reinforcement
embryo.
Thus,
bidirectional
molecular
dialogue
between
endosperm
safeguards
integrity
before
germination.
