The Plant Journal,
Год журнала:
2024,
Номер
120(4), С. 1591 - 1604
Опубликована: Окт. 12, 2024
SUMMARY
The
cuticle
is
the
first
physical
barrier
covering
surface
of
tomatoes
and
plays
an
important
role
in
multiple
stress
responses.
But
molecular
regulatory
networks
formation
are
not
fully
understood.
In
this
study,
we
found
that
SlMYB72
can
interact
with
SlTAGL1
to
regulate
fruit
tomato.
Downregulating
expression
inhibits
cuticle,
resulting
a
reduced
thickness,
accelerated
postharvest
water
loss,
increased
susceptibility
Botrytis
cinerea
.
RNA
sequencing
analysis
showed
downregulation
gene
decreased
levels
genes
related
fatty
acid
metabolism.
regulates
by
directly
binding
promoter
long‐chain
acyl‐coA
synthetases
(
SlLACS1
)
medium‐chain
alkane
hydroxylase
SlMAH1
).
Moreover,
interacts
SlTAGL1,
which
enhance
transcriptional
activation
on
promoter.
Overall,
our
study
expands
understanding
regulation
provides
new
insights
into
shelf
life
extension
via
manipulation
content.
New Phytologist,
Год журнала:
2021,
Номер
231(5), С. 2050 - 2064
Опубликована: Май 27, 2021
Summary
Plant
glandular
secretory
trichomes
(GSTs)
produce
various
specialized
metabolites.
Increasing
GST
density
represents
a
strategy
to
enhance
the
yield
of
these
chemicals;
however,
gene
regulatory
network
that
controls
initiation
remains
unclear.
In
previous
study
Artemisia
annua
L.,
we
found
HD‐ZIP
IV
transcription
factor,
AaHD1,
promotes
by
directly
regulating
AaGSW2
.
Here,
identified
two
AaHD1‐interacting
factors,
namely
AaMIXTA‐like
2
(AaMYB16)
and
AaMYB5.
Through
generation
characterization
transgenic
plants,
AaMYB16
is
positive
regulator
initiation,
whereas
AaMYB5
has
opposite
effect.
Notably,
neither
them
regulates
formation
independently.
Rather,
they
act
competitively,
interacting
modulating
AaHD1
promoter
binding
activity.
Additionally,
phytohormone
jasmonic
acid
(JA)
was
shown
be
associated
with
AaHD1‐AaMYB16/AaMYB5
through
transcriptional
regulation
via
JASMONATE‐ZIM
DOMAIN
(JAZ)
protein
repressor.
These
results
bring
new
insights
into
mechanism
complexes,
which
appear
have
similar
functions
in
range
vascular
plant
taxa.
The Plant Cell,
Год журнала:
2023,
Номер
35(6), С. 2251 - 2270
Опубликована: Фев. 21, 2023
Abstract
The
plant
cuticle,
a
structure
primarily
composed
of
wax
and
cutin,
forms
continuous
coating
over
most
aerial
surfaces.
cuticle
plays
important
roles
in
tolerance
to
environmental
stress,
including
stress
imposed
by
drought.
Some
members
the
3-KETOACYL-COA
SYNTHASE
(KCS)
family
are
known
act
as
metabolic
enzymes
involved
cuticular
production.
Here
we
report
that
Arabidopsis
(Arabidopsis
thaliana)
KCS3,
which
was
previously
shown
lack
canonical
catalytic
activity,
instead
functions
negative
regulator
metabolism
reducing
enzymatic
activity
KCS6,
key
KCS
We
demonstrate
role
KCS3
regulating
KCS6
involves
physical
interactions
between
specific
subunits
fatty
acid
elongation
complex
is
essential
for
maintaining
homeostasis.
also
show
KCS3–KCS6
module
synthesis
highly
conserved
across
diverse
taxa
from
moss
Physcomitrium
patens,
pointing
critical
ancient
basal
function
this
finely
synthesis.
Agronomy,
Год журнала:
2020,
Номер
10(3), С. 411 - 411
Опубликована: Март 19, 2020
The
tomato
(Solanum
lycopersicum)
is
one
of
the
most
popular
vegetables
in
world.
In
production,
due
to
effects
diseases,
insect
pests,
drought,
and
cold
damage,
large-scale
production
reduction
often
caused.
Plant
trichomes
are
protruding
attachments
distributed
on
surface
different
plants,
providing
protection
for
plants.
When
plant
under
external
stress,
can
play
an
important
role
protecting
from
damage
through
its
physical
structure.
density
type
closely
related
stress
resistance
tomatoes.
wo
mutant
LA3186
(referred
herein
as
“3186M”),
“3186L”),
ln
LA3-071
“3-071”),
cultivar
Jia
Ren
“JR”,
used
control),
which
possess
numbers
leaves,
were
materials;
glandular
characteristics,
types,
densities
observed
a
scanning
electron
microscope
(SEM);
transmission
microscopy
(TEM)
was
observe
subcellular
structure
leaves.
relationship
between
investigated
with
treatments
low
temperature,
disease,
insects.
This
study
provides
theoretical
foundation
practical
basis
further
utilization
regulation
trichome-related
characteristics
Molecular Horticulture,
Год журнала:
2022,
Номер
2(1)
Опубликована: Янв. 20, 2022
Abstract
As
the
most
valuable
organ
of
tomato
plants,
fruit
has
attracted
considerable
attention
which
focus
on
its
quality
formation
during
ripening
process.
A
amount
research
reported
that
is
affected
by
metabolic
shifts
are
under
coordinated
regulation
both
structural
genes
and
transcriptional
regulators.
In
recent
years,
with
development
next
generation
sequencing,
molecular
genetic
analysis
methods,
lots
involved
in
chlorophyll,
carotenoid,
cell
wall,
central
secondary
metabolism
have
been
identified
confirmed
to
regulate
pigment
contents,
softening
other
aspects
flavor
quality.
Here,
concerning
dissection
related
changes,
post-translational
these
pathways
reviewed.
Furthermore,
a
weighted
gene
correlation
network
representative
carried
out
potential
combined
application
analysis,
fine-mapping
strategies
sequencing
identify
novel
candidate
determinants
discussed.
Horticulture Research,
Год журнала:
2024,
Номер
11(3)
Опубликована: Янв. 31, 2024
Tomato
(Solanum
lycopersicum)
is
a
globally
cultivated
crop
with
great
economic
value.
The
exocarp
determines
the
appearance
of
tomato
fruit
and
protects
it
from
various
biotic
abiotic
challenges
at
both
pre-harvest
post-harvest
stages.
However,
no
exocarp-specific
promoter
currently
available,
which
hinders
exocarp-based
genetic
engineering.
Here,
we
identified
by
RNA
sequencing
reverse
transcription-quantitative
PCR
analyses
that
gene
SlPR10
(PATHOGENESIS
RELATED
10)
was
abundantly
predominantly
expressed
in
exocarp.
A
fluorescent
reporter
2087-bp
(pSlPR10)
mainly
detected
transgenic
plants
Ailsa
Craig
Micro-Tom
cultivars.
This
further
utilized
for
expression
SlANT1
SlMYB31
tomato,
are
master
regulators
anthocyanin
cuticular
wax
biosynthesis,
respectively.
pSlPR10-driven
resulted
accumulation
exocarp,
conferring
gray
mold
resistance
extended
shelf
life
to
fruit,
while
led
waxy
thickening
skin,
delaying
water
loss
also
extending
life.
Intriguingly,
pSlPR10
two
other
weaker
exocarp-preferential
promoters
exhibited
coincided
specificities
gynophore
Arabidopsis
(Arabidopsis
thaliana)
plants,
providing
not
only
an
inkling
evolutionary
homology
between
but
useful
studying
biology
Arabidopsis.
Collectively,
this
work
reports
desirable
enabling
targeted
demonstrates
its
usefulness
improvement
quality.
Molecular Horticulture,
Год журнала:
2024,
Номер
4(1)
Опубликована: Март 19, 2024
Abstract
Artemisinin
is
primarily
synthesized
and
stored
in
the
subepidermal
space
of
glandular
trichomes
Artemisia
annua
.
The
augmentation
trichome
density
has
been
demonstrated
to
enhance
artemisinin
yield.
However,
existing
literature
lacks
insights
into
correlation
between
stratum
corneum
trichomes.
This
study
aims
unravel
involvement
TrichomeLess
Regulator
3
(
TLR3
),
which
encodes
transcription
factor,
biosynthesis
its
potential
association
with
corneum.
was
identified
as
a
candidate
gene
through
transcriptome
analysis.
role
development
morphology
investigated
using
yeast
two-hybrid,
pull-down
analysis,
RNA
electrophoresis
mobility
assay.
Our
research
revealed
that
negatively
regulates
development.
It
modulates
Arabidopsis
thaliana
by
inhibiting
branching
inducing
formation
abnormal
Overexpression
disrupts
arrangement
reduces
content.
Simultaneously,
possesses
capacity
regulate
follicle
interacting
TRICHOME
AND
ARTEMISININ
REGULATOR
1,
CycTL.
Consequently,
our
findings
underscore
pivotal
biosynthesis,
thereby
influencing
