Emerging roles and mechanisms of lncRNAs in fruit and vegetables

Horticulture Research, Год журнала: 2024, Номер 11(4)

Опубликована: Фев. 23, 2024

With the development of genome sequencing technologies, many long non-coding RNAs (lncRNAs) have been identified in fruit and vegetables. lncRNAs are primarily transcribed spliced by RNA polymerase II (Pol II) or plant-specific Pol IV/V, exhibit limited evolutionary conservation. intricately regulate various aspects vegetables, including pigment accumulation, reproductive tissue development, ripening, responses to biotic abiotic stresses, through diverse mechanisms such as gene expression modulation, interaction with hormones transcription factors, microRNA regulation, involvement alternative splicing. This review presents a comprehensive overview lncRNA classification, basic characteristics, and, most importantly, recent advances understanding their functions regulatory mechanisms.

Язык: Английский

---

After silencing suppression: miRNA targets strike back

Trends in Plant Science, Год журнала: 2024, Номер 29(11), С. 1266 - 1276

Опубликована: Май 28, 2024

Язык: Английский

---

The regulatory roles of plant miRNAs in biotic stress responses

Biochemical and Biophysical Research Communications, Год журнала: 2025, Номер 755, С. 151568 - 151568

Опубликована: Март 1, 2025

Язык: Английский

---

Combination of PAMP‐induced peptide signaling and its regulator SpWRKY65 boosts tomato resistance to Phytophthora infestans

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

SUMMARY Late blight, caused by Phytophthora infestans ( P. ), seriously compromises tomato growth and yield. PAMP‐induced peptides (PIPs) are secreted that act as endogenous elicitors, triggering plant immune responses. Our previous research indicated the exogenous application of PIP1 from Solanum pimpinelifolium L3708, named SpPIP1, enhances resistance to . However, little is known about roles additional family members in In addition, there remains a significant gap understanding receptors SpPIPs transcription factors (TFs) regulate signaling defense, combination TFs defending against pathogens rarely studied. This study demonstrates SpPIP‐LIKE1 (SpPIPL1) also strengthens affecting phenylpropanoid biosynthesis pathway. Both SpPIP1 SpPIPL1 trigger defense responses manner dependent on RLK7L. Tomato plants overexpressing precursors (SpprePIP1 SpprePIPL1) exhibited enhanced expression pathogenesis‐related genes, elevated H 2 O ABA levels, increased lignin accumulation. Notably, SpWRKY65 was identified transcriptional activator SpprePIP1 SpprePIPL1 Disease assays gene analyses revealed overexpression (OEWRKY65) confers , while wrky65 knockout led opposite effect. Intriguingly, transgenic studies showed either spraying OEWRKY65 with or co‐overexpressing further augmented resistance, underscoring potential stacking enhancing disease resistance. summary, this offers new perspectives controlling late blight developing varieties improved The results emphasize an eco‐friendly strategy for crop protection, laying theoretical foundation advancing breeding.

Язык: Английский

---

Long Non-Coding RNAs, the Dark Matter: An Emerging Regulatory Component in Plants

International Journal of Molecular Sciences, Год журнала: 2020, Номер 22(1), С. 86 - 86

Опубликована: Дек. 23, 2020

Long non-coding RNAs (lncRNAs) are pervasive transcripts of longer than 200 nucleotides and indiscernible coding potential. lncRNAs implicated as key regulatory molecules in various fundamental biological processes at transcriptional, post-transcriptional, epigenetic levels. Advances computational experimental approaches have identified numerous plants. been found to act prime mediators plant growth, development, tolerance stresses. This review summarizes the current research status planta, their classification based on genomic context, mechanism action, specific bioinformatics tools resources for identification characterization. Our overarching goal is summarize recent progress understanding role developmental such flowering time, reproductive abiotic We also lncRNA nutrient stress ability improve biotic Given pivotal processes, functional characterization agriculturally essential crop plants crucial bridging gap between phenotype genotype.

Язык: Английский

---

Interactions and links among the noncoding RNAs in plants under stresses

Theoretical and Applied Genetics, Год журнала: 2020, Номер 133(12), С. 3235 - 3248

Опубликована: Окт. 6, 2020

Язык: Английский

---

Late blight in tomato: insights into the pathogenesis of the aggressive pathogen Phytophthora infestans and future research priorities

Planta, Год журнала: 2021, Номер 253(6)

Опубликована: Май 8, 2021

Язык: Английский

---

Function identification of miR394 in tomato resistance to Phytophthora infestans

Plant Cell Reports, Год журнала: 2021, Номер 40(10), С. 1831 - 1844

Опубликована: Июль 6, 2021

Язык: Английский

---

Bacillus subtilis SL18r Induces Tomato Resistance Against Botrytis cinerea, Involving Activation of Long Non-coding RNA, MSTRG18363, to Decoy miR1918

Frontiers in Plant Science, Год журнала: 2021, Номер 11

Опубликована: Фев. 3, 2021

Mounting evidence has indicated that beneficial rhizobacteria can suppress foliar pathogen invasion via elicitation of induced systemic resistance (ISR). However, it remains elusive whether long non-coding RNAs (lncRNAs) are involved in the mediation rhizobacteria-primed ISR processes plants. Herein, we demonstrated ability rhizobacterial strain Bacillus subtilis SL18r to trigger tomato plants against Botrytis cinerea . Comparative transcriptome analysis was conducted screen differentially expressed lncRNAs (DELs) between non-inoculated and SL18r-inoculated Among these DELs, four variants MSTRG18363 possessed conserved binding sites for miR1918, which negatively regulates immune systems The expression leaves significantly by inoculation. transcription correlated with but displayed a positive correlation RING-H2 finger gene SlATL20 (a target miR1918). Moreover, MSTRG18363-overexpressing exhibited enhanced disease resistance, reduction miR1918 transcripts, marked increases expression. SL18r-induced largely impaired MSTRG18363-silenced VIGS-mediated silencing also greatly weakened resistance. Collectively, our results suggested induction conducive promoting decoy regulating , thereby provoking responses infection.

Язык: Английский

---

NaWRKY3 is a master transcriptional regulator of the defense network against brown spot disease in wild tobacco

Journal of Experimental Botany, Год журнала: 2023, Номер 74(14), С. 4169 - 4188

Опубликована: Апрель 24, 2023

Abstract WRKY transcription factors are involved in plant defense against pathogens. No WRKYs have been reported to be resistance tobacco brown spot disease caused by Alternaria alternata. Here, we found that NaWRKY3 plays a critical role Nicotiana attenuata A. bound and regulated many genes, including: lipoxygenase 3, ACC synthase 1, oxidase three jasmonate- ethylene-biosynthetic genes; feruloyl-CoA 6ʹ-hydroxylase 1 (NaF6ʹH1), the biosynthetic gene for phytoalexins scopoletin scopolin; alternata long non-coding RNA L2, NADPH (NaRboh D), berberine bridge-like (NaBBL28). Silencing L2 reduced jasmonate concentrations NaF6ʹH1 expression. NaRboh D-silenced plants were severely impaired reactive oxygen species production stomatal closure responses. NaBBL28 was first BBL identified hydroxylation of 17-hydroxygeranyllinalool diterpene glycosides. its own promoter but repressed Thus, demonstrated is fine-tuned master regulator network N. regulating several signaling pathways metabolites. This time such an important has species, providing new insights into

Язык: Английский

---