Methods in enzymology on CD-ROM/Methods in enzymology, Год журнала: 2022, Номер unknown, С. 279 - 303
Опубликована: Янв. 1, 2022
Язык: Английский
Plant Direct, Год журнала: 2024, Номер 8(8)
Опубликована: Авг. 1, 2024
Light/dark (LD) cycles are responsible for oscillations in gene expression, which modulate several aspects of plant physiology. Those can persist under constant conditions due to regulation by the circadian oscillator. The response transcriptome light regimes is dynamic and allows plants adapt rapidly changing environmental conditions. We compared Arabidopsis LD (LL) 3 days identified different co-expression networks two regimes. Our studies yielded unforeseen insights into regulation. Intuitively, we anticipated that clusters regulated oscillator would display cycles. However, found transcripts encoding components flavonoid metabolism pathway were rhythmic LL but not LD. also discovered expressions many stress-related genes significantly increased during dark period relative subjective night LL, whereas expression these was similar. nocturnal pattern suggested a form "skotoprotection." transcriptomics data made available web application named
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 30, 2024
Abstract Cadmium (Cd) is a major environmental pollutant with high toxicity potential. Even though reduction of growth, including the primary root, clear consequence Cd exposure, profound understanding impact on root apical meristem (RAM) and elongation/differentiation zone (EDZ) still lacking. In this study, Arabidopsis thaliana roots were subjected to divided into tips (RT) remaining (RR) separately assess effect using transcriptomics, ionomics metabolomics. Elemental profiling revealed lower accumulation in RT differences mineral contents between RR. Transcriptomic analysis demonstrated distinct gene expression patterns RR, having less RT. Functional enrichment genes associated iron sulfur homeostasis as well response light both RR RT-specific responses included several regulated by transcription factor ELONGATED HYPOCOTYL 5 (HY5) notably, hy5 mutant showed increased sensitivity compared wild type. This study provides comprehensive insights inhibitory effects elucidating molecular mechanisms involved, particularly highlighting role HY5 accumulation.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 31, 2024
Summary Zinc (Zn) excess negatively impacts primary root growth in Arabidopsis. Yet, the effects of Zn on specific processes tip remain largely unexplored. Transcriptomics, ionomics and metabolomics were used to examine impact (RT) compared remaining (RR). exposure resulted shortened apical meristem elongation zone, with differentiation initiating closer root. accumulated at a lower concentration RT than RR. This pattern was associated expression homeostasis Fe deficiency response genes. A distinct distribution RR highlighted by Laser Ablation ICP-MS analysis. Specialized Trp-derived metabolism genes, typically redox biotic stress responses, specifically up-regulated upon excess, among those Phytoalexin Deficient 3 ( PAD3 ) encoding last enzyme camalexin synthesis. In roots wild-type seedlings, increased 6-fold pad3 mutant displayed sensitivity an altered ionome. Our results indicate that iron mechanisms are key elements RT.
Язык: Английский
Процитировано
0
Methods in enzymology on CD-ROM/Methods in enzymology, Год журнала: 2022, Номер unknown, С. 279 - 303
Опубликована: Янв. 1, 2022
Язык: Английский
Процитировано
1