Research Square (Research Square),
Год журнала:
2022,
Номер
unknown
Опубликована: Авг. 19, 2022
Abstract
Background:
Long
non-coding
RNAs
(lncRNAs),
as
important
regulators,
play
roles
in
plant
growth
and
development.
The
expression
epigenetic
regulation
of
lncRNAs
remain
uncharacterized
generally
seeds,
especially
the
transient
endosperm
dicotyledons.
Results:
In
this
study,
we
identified
11,840
candidate
12
day-after-pollination
sunflower
by
analyzing
RNA-seq
data.
These
were
evenly
distributed
all
chromosomes
had
specific
features
that
distinct
from
mRNAs
including
tissue-specificity
expression,
shorter
fewer
exons.
By
GO
analysis
protein
coding
genes
showing
strong
correlation
with
lncRNAs,
revealed
these
potential
function
many
biological
processes
seed
Additionally,
genome-wide
DNA
methylation
analyses
level
at
transcription
start
sites
was
negatively
correlated
gene
levels
lncRNAs.
Finally,
36
imprinted
32
maternally
expressed
four
paternally
CG
CHG
context,
upstream
body
regions
slightly
lower
than
embryo
tissues,
which
indicated
maternal
demethylation
potentially
induce
bias
endosperm.
Conclusion:
Our
findings
not
only
characterized
on
a
scale
development
endosperm,
but
also
provide
novel
insights
into
parental
effects
dicotyledonous
seeds.
Biotechnology Advances,
Год журнала:
2023,
Номер
69, С. 108248 - 108248
Опубликована: Сен. 2, 2023
Cereal
crops,
including
triticeae
species
(barley,
wheat,
rye),
as
well
edible
cereals
(wheat,
corn,
rice,
oat,
rye,
sorghum),
are
significant
suppliers
for
human
consumption,
livestock
feed,
and
breweries.
Over
the
past
half-century,
modern
varieties
of
cereal
crops
with
increased
yields
have
contributed
to
global
food
security.
However,
presently
cultivated
elite
crop
were
developed
mainly
optimal
environmental
conditions.
Thus,
it
has
become
evident
that
taking
into
account
ongoing
climate
changes,
currently
a
priority
should
be
given
developing
new
stress-tolerant
cultivars.
It
is
necessary
enhance
accuracy
methods
time
required
generate
cultivars
desired
features
adapt
change
keep
up
world
population
expansion.
The
CRISPR/Cas9
system
been
powerful
versatile
genome
editing
tool
achieve
desirable
traits,
such
high-yielding,
stress-tolerant,
disease-resistant
transgene-free
lines
in
major
cereals.
Despite
recent
advances,
application
faces
several
challenges,
amount
develop
lines,
laboriousness,
limited
number
genotypes
may
used
transformation
vitro
regeneration.
Additionally,
through
restricted
many
countries,
especially
Europe
New
Zealand,
due
lack
flexibility
GMO
regulations.
This
review
provides
comprehensive
update
researchers
interested
improving
using
gene-editing
technologies,
CRISPR/Cas9.
We
will
some
critical
studies
on
improvements
their
contributing
factors
superior
technologies.
Pathogens,
Год журнала:
2025,
Номер
14(3), С. 256 - 256
Опубликована: Март 5, 2025
Long
non-coding
RNAs
(lncRNAs)
represent
a
class
of
RNAs.
In
the
study
Pseudomonas
putida
Sneb821-induced
tomato
resistance
to
Meloidogyne
incognita,
reverse
transcription
polymerase
chain
reaction
(RT-PCR)
was
employed
validate
12
lncRNAs
in
tomato.
Among
them,
lncRNA47258/miR319b/TCP
molecular
regulatory
module
likely
implicated
process
against
M.
incognita.
Through
application
hairy
root
and
virus-induced
gene
silencing
(VIGS)
technologies
for
investigation
lncRNA47258,
it
determined
that
lncRNA47258
could
target
TCP
(Solyc07g062681.1)
modulate
metabolic
pathway
jasmonic
acid-related
indices,
thereby
impeding
infection
Moreover,
overexpression
using
technology
demonstrated
regulate
acid
synthesis
tomato,
consequently
obstructing
suppressing
development
Collectively,
preliminarily
verified
be
involved
incognita
PLANT PHYSIOLOGY,
Год журнала:
2024,
Номер
195(2), С. 940 - 957
Опубликована: Фев. 28, 2024
Long
noncoding
RNAs
(lncRNAs)
play
important
roles
in
various
biological
processes.
However,
the
regulatory
of
lncRNAs
underlying
fruit
development
have
not
been
extensively
studied.
The
pumpkin
(Cucurbita
spp.)
is
a
preferred
model
for
understanding
molecular
mechanisms
regulating
because
its
variable
shape
and
size
large
inferior
ovary.
Here,
we
performed
strand-specific
transcriptome
sequencing
on
maxima
"Rimu")
fruits
at
6
developmental
stages
identified
5,425
reliably
expressed
lncRNAs.
Among
332
that
were
differentially
during
development,
lncRNA
MSTRG.44863.1
was
as
negative
regulator
development.
showed
relatively
high
expression
level
an
obvious
period-specific
pattern.
Transient
overexpression
silencing
significantly
increased
decreased
content
1-aminocyclopropane
carboxylic
acid
(a
precursor
ethylene)
ethylene
production,
respectively.
RNA
pull-down
microscale
thermophoresis
assays
further
revealed
can
interact
with
S-adenosyl-L-methionine
synthetase
(SAMS),
enzyme
synthesis
pathway.
Considering
negatively
regulates
these
results
indicate
plays
role
regulation
possibly
through
interacting
SAMS
affecting
synthesis.
Overall,
our
findings
provide
rich
resource
study
fruit-related
while
offering
insights
into
plants.
Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Окт. 26, 2023
Eukaryotic
genomes
encode
thousands
of
RNA
molecules;
however,
only
a
minimal
fraction
is
translated
into
proteins.
Among
the
non-coding
elements,
long
RNAs
(lncRNAs)
play
important
roles
in
diverse
biological
processes.
LncRNAs
are
associated
mainly
with
regulation
expression
genome;
nonetheless,
their
study
has
just
scratched
surface.
This
somewhat
due
to
lack
widespread
conservation
at
sequence
level,
addition
relatively
low
and
highly
tissue-specific
patterns,
which
makes
exploration
challenging,
especially
plant
where
few
these
molecules
have
been
described
completely.
Recently
published
high-quality
crop
plants,
along
new
computational
tools,
considered
promising
resources
for
studying
plants.
review
briefly
summarizes
characteristics
lncRNAs,
presence
conservation,
different
protocols
find
limitations
protocols.
Likewise,
it
describes
physiological
phenomena.
We
believe
that
lncRNAs
can
help
design
strategies
reduce
negative
effect
biotic
abiotic
stresses
on
yield
plants
and,
future,
create
fruits
vegetables
improved
nutritional
content,
higher
amounts
compounds
positive
effects
human
health,
better
organoleptic
characteristics,
longer
postharvest
shelf
life.
Journal of Experimental Botany,
Год журнала:
2023,
Номер
74(19), С. 6158 - 6175
Опубликована: Авг. 7, 2023
Abstract
The
development
of
the
clustered
regularly
interspaced
short
palindromic
repeats/CRISPR-associated
protein
(CRISPR/Cas)
system
has
provided
precise
and
efficient
strategies
to
edit
target
genes
generate
transgene-free
crops.
Significant
progress
been
made
in
editing
protein-coding
genes;
however,
studies
on
non-coding
DNA
with
regulatory
roles
lags
far
behind.
Non-coding
DNAs,
including
those
which
can
be
transcribed
into
long
RNAs
(lncRNAs),
miRNAs,
together
cis-regulatory
elements
(CREs),
play
crucial
regulating
plant
growth
development.
Therefore,
combination
CRISPR/Cas
technology
great
potential
novel
alleles
that
affect
various
agronomic
traits
crops,
thus
providing
valuable
genetic
resources
for
crop
breeding.
Herein,
we
review
recent
advances
DNA,
attempts
improvement,
application
tools
modulating
DNA.
Finally,
existing
problems,
possible
solutions,
future
applications
gene
modern
breeding
practice
are
also
discussed.
Journal of Agricultural and Food Chemistry,
Год журнала:
2023,
Номер
71(43), С. 15895 - 15907
Опубликована: Окт. 20, 2023
Plant
height
is
an
important
agronomic
trait
that
closely
associated
with
crop
yield
and
quality.
Gibberellins
(GAs),
a
class
of
highly
efficient
plant
growth
regulators,
play
key
roles
in
regulating
height.
Increasing
reports
indicate
transcriptional
regulation
major
point
the
GA
pathways.
Although
substantial
knowledge
has
been
gained
regarding
biosynthetic
signaling
pathways,
factors
contributing
to
regulatory
mechanisms
homeostatically
controlling
levels
remain
be
elucidated.
Here,
we
provide
overview
current
network
involving
transcription
factors,
noncoding
RNAs,
histone
modifications
involved
We
also
discuss
interaction
between
GAs
other
hormones
development.
Finally,
future
directions
for
applying
hormone
breeding
are
described.
