Abstract
miR156
play
important
roles
in
regulation
of
plant
growth
and
development,
secondary
metabolite
synthesis
other
biological
processes
by
targeting
the
SQUAMOSA
promoter
binding
protein-like
(SPL)
family.
Our
previous
sequencing
data
analysis
suggested
that
Csn-miR156d
may
regulate
flowering
anthocyanin
accumulation
cleavage
degradation
expression
SPL
tea
plant,
but
it
remains
to
be
elucidated.
In
this
study,
5′RLM-RACE
experiment,
tobacco
transient
transformation,
qRT-PCR
antisense
oligonucleotide
(asODN)
were
used
verify
CsSPL1
is
target
gene
Csn-miR156d.
Stable
transformation
Arabidopsis
revealed
could
delay
negatively
regulating
transcript
levels
FT,
AP1,
FUL
SOC1,
while
overexpression
showed
an
opposite
effect.
Additionally,
enhance
transcription
biosynthesis-related
structural
genes
DFR,
ANS,
F3H,
UGT78D2
LDOX,
as
well
regulatory
PAP1,
MYB113,
GL3,
MYB11
MYB12,
leading
accumulation.
Moreover,
asODN
experiment
increase
content
plant.
These
results
suggest
regulates
suppressing
CsSPL1.
study
provides
new
insights
into
development
lays
a
theoretical
foundation
for
further
research
on
molecular
mechanism
miRNAs
metabolism.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(4), С. 2096 - 2096
Опубликована: Фев. 8, 2024
Basic
helix–loop–helix
(bHLH)
transcription
factors
extensively
affect
various
physiological
processes
in
plant
metabolism,
growth,
and
abiotic
stress.
However,
the
regulation
mechanism
of
bHLH
balancing
anthocyanin
biosynthesis
stress
sweet
potato
(Ipomoea
batata
(L.)
Lam.)
remains
unclear.
Previously,
transcriptome
analysis
revealed
genes
that
were
differentially
expressed
among
purple-fleshed
cultivar
‘Jingshu
6’
its
anthocyanin-rich
mutant
‘JS6-5’.
Here,
we
selected
one
these
potential
genes,
IbMYC2,
which
belongs
to
factor
family,
for
subsequent
analyses.
The
expression
IbMYC2
JS6-5
storage
roots
is
almost
four-fold
higher
than
Jingshu
6
significantly
induced
by
hydrogen
peroxide
(H2O2),
methyl
jasmonate
(MeJA),
NaCl,
polyethylene
glycol
(PEG)6000.
Overexpression
enhances
production
exhibits
a
certain
antioxidant
capacity,
thereby
improving
salt
drought
tolerance.
In
contrast,
reducing
increases
susceptibility.
Our
data
showed
could
elevate
synthesis
pathway
binding
IbCHI
IbDFR
promoters.
Additionally,
overexpressing
activates
encoding
reactive
oxygen
species
(ROS)-scavenging
proline
enzymes
under
conditions.
Taken
together,
results
demonstrate
gene
exercises
significant
impact
on
crop
quality
resistance.
Molecular Horticulture,
Год журнала:
2025,
Номер
5(1)
Опубликована: Фев. 3, 2025
Abstract
5-Aminolevulinic
acid
(ALA),
as
a
natural
plant
growth
regulator,
is
well
known
for
promoting
red
fruit
coloring
by
enhancing
anthocyanin
accumulation.
However,
the
underlying
mechanisms
remain
elusive.
In
this
study,
we
firstly
demonstrated
that
ALA
upregulates
gene
expression
of
transcription
factor
MdMADS1,
which
in
turn
directly
binds
to
and
activates
key
biosynthetic
genes,
MdCHS
MdUFGT
.
Then,
identified
novel
WRKY
factor,
MdWRKY71,
interacts
with
MdMADS1.
Through
manipulation,
revealed
MdWRKY71
plays
pivotal
role
ALA-induced
accumulation,
highlighting
its
regulatory
significance
process.
Further
investigation
unveiled
not
only
MdMADS1
but
also
enhances
transcriptional
activation
on
target
Additionally,
discovered
independently
two
other
MdANS
MdDFR
The
protein–protein
interaction
between
amplifies
these
genes
MdWRKY71.
These
findings
delineate
fine
complex
framework
where
coordinately
regulate
biosynthesis
apples,
providing
new
insights
into
molecular
control
coloration
offering
potential
breeding
aimed
at
quality.
SWEET
(Sugar
Will
Eventually
be
Exported
Transporter)
proteins
play
vital
roles
in
the
transport
of
sugars,
contributing
to
regulation
plant
development,
hormone
signaling,
and
responses
abiotic
stress.
In
this
study,
we
identified
57
NtSWEETs
tobacco
(Nicotiana
tabacum
L.),
then
their
physicochemical
properties,
chromosomal
localization,
synteny,
phylogenetic
relationships,
genomic
structure,
promoter
cis-elements,
protein
interaction
network,
tissue
specificity,
expression
pattern
were
systematically
analyzed.
addition,
NtSWEET12i
improves
drought
saline-alkali
tolerance
by
enhancing
soluble
sugars
transport,
ABA
proline
biosynthesis,
ROS
scavenging.
These
findings
illuminate
role
NtSWEETs,
particularly
NtSWEET12i,
regulating
stresses.
This
study
offers
new
insights
enhance
our
understanding
identify
potential
genes
involved
plants.
Not
applicable.
