Integrated Transcriptomics and Metabolomics Reveal Key Genes and Metabolic Pathway in Flower and Fruit Color Formation of Cerasus humilis (Bge.) Sok

Plants, Год журнала: 2025, Номер 14(7), С. 1103 - 1103

Опубликована: Апрель 2, 2025

Anthocyanins play a pivotal role in determining the color diversity flowers and fruits of Cerasus humilis (Bge.) Sok. This study performed metabolomic analysis two varieties differing pigmentation phenotypes ('Jinou 1' 'Nongda 5'), results indicated that cyanidin, pelargonidin, paeonidin, delphinidin were main substances serving as primary pigments contributing to their striking chromatic divergence between varieties. Transcriptome profiling revealed several key structural genes (ChCHS1, ChDFR, ChF3H, ChF3'H) anthocyanin biosynthesis pathway exhibited significantly elevated expression levels 'Jinou compared 5'. Further transcriptomic correlation analyses identified ChMYB9 ChMYB12 strong positive associations with metabolites both floral fruit tissues. Notably, displayed strongest metabolite profiles, suggesting it may serve core regulatory component biosynthesis. research provides new insights into mechanisms C. humilis.

Язык: Английский

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Sugar transporters: mediators of carbon flow between plants and microbes

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Апрель 16, 2025

Pathogens and symbiotic microorganisms significantly influence plant growth crop productivity. Enhancing disease resistance maximizing the beneficial role of in agriculture constitute critical areas scientific investigation. A fundamental aspect plant-microorganisms interactions revolves around nutritional dynamics, characterized by either "food shortage" or supply" scenarios. Notably, pathogenic predominantly utilize photosynthetic sugars as their primary carbon source during host colonization. This phenomenon has generated substantial interest regulatory mechanisms governing sugar transport redistribution at plant-microorganism interface. Sugar transporters, which primarily mediate allocation to various sink organs, have emerged crucial players plant-pathogen establishment associations. review systematically categorized transporters highlighted functional significance mediating with microorganisms. Furthermore, we synthesized recent advancements understanding molecular these identified key questions warranting further Elucidating roles offers novel strategies for enhancing health productivity, thereby contributing agricultural sustainability global food security.

Язык: Английский

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MYB transcription factor MdMYB44 positively regulates fruit crispness by directly activating the expression of pectin methylesterase MdMPE3 in apple

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 224, С. 109936 - 109936

Опубликована: Апрель 17, 2025

Язык: Английский

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CsbHLH122/CsMYBS3‐CsSUT2 contributes to the rapid accumulation of sugar in the ripening stage of sweet orange (Citrus sinensis)

The Plant Journal, Год журнала: 2025, Номер 122(1)

Опубликована: Апрель 1, 2025

SUMMARY The sugar content serves as the fundamental metabolic component that determines both flavor quality and nutritional value of fruits. Nevertheless, regulatory mechanism underlying rapid accumulation sugars during citrus fruit maturation remains elusive. In this study, we demonstrated expression level sucrose transporter CsSUT2 is increased ripening accumulation. Functional assays confirmed localized in plasma membrane exhibits activity. Homologous heterologous overexpression juice sacs, calli, tomato resulted an increase content. Conversely, virus‐induced gene silencing RNAi‐mediated led to a decrease levels transgenic tissues. We further identified CsMYBS3 upstream transcription factor positively regulates . Transgenic evidence supported induction by depends on transcript Additionally, found CsbHLH122 physically interacts with form complex, enhancing promoter transcriptional activity This study expands our understanding function citrus, providing valuable insights for regulating control fruit.

Язык: Английский

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Multi-omics analysis provides new insights into the changes of important nutrients and fructose metabolism in loquat bud sport mutant

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 28, 2024

Bud sport is a common and stable somatic variation in perennial fruit trees, often leads to significant modification of traits affects the breeding value. To investigate impact bud on main metabolites white-fleshed loquat, we conducted multi-omics analysis loquat fruits at different developmental stages mutant Dongting (TBW) its wild type (TBY). The findings from detection quality indices suggested that resulted reduction accumulation carotenoids, fructose, titratable acid terpenoids mature stage TBW, while leading flavonoids, phenolic acids, amino acids lipids. comparably low content further enhances balanced pleasent taste profile TBW. Expression patterns differentially expressed genes involved fructose metabolism exhibited increase expression level S6PDH ( EVM0006243 , EVM0044405 ) prior maturation. comparison protein sequences promoter region between TBY TBW revealed no structural variations would gene function or expression, indicating transcription factors may be responsible for rapid up-regulation before Furthermore, correlation helped construct comprehensive regulatory network including 23 factors, six genes, nine saccharides. Based existing studies, it could inferred such as ERF, NAC, MYB, GRAS, bZIP promote flesh by positively regulating . These improve our understanding nutritional value potential mutant, well serve foundation exploring regulate loquat.

Язык: Английский

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Comparative transcriptome analysis identifies candidate genes related to sucrose accumulation in longan (Dimocarpus longan Lour.) pulp

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 5, 2024

Sucrose content is one of the important factors to determine longan fruit flavor quality. To gain deep insight molecular mechanism on sucrose accumulation in longan, we conducted comparative transcriptomic analysis between low cultivar ‘Qingkebaoyuan’ and high ‘Songfengben’. A total 12,350 unique differentially expressed genes (DEGs) were detected across various development stages different varieties, including hexokinase (HK) sucrose-phosphate synthase (SPS), which are intricately linked soluble sugar metabolism. Weighted gene co-expression network (WGCNA) identified magenta module, DlSPS gene, was significantly positively correlated with content. Furthermore, transient expression unveiled play crucial role accumulation. Moreover, 5 transcription (MYB, ERF, bHLH, C2H2, NAC) potentially involved regulation. Our findings provide clues for metabolism, lay foundation breeding future.

Язык: Английский

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An anthocyanin activation gene underlies the purple central flower pigmentation in wild carrot

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 196(2), С. 1147 - 1162

Опубликована: Июль 24, 2024

Abstract Many organisms have complex pigmentation patterns. However, how these patterns are formed remains largely unknown. In wild carrot (Daucus carota subsp. carota), which is also known as Queen Anne's lace, one or several purple central flowers occur in white umbels. Here, we investigated the unique flower pattern Using and cultivated (D. sativus L.) accessions, transcriptome analysis, protein interaction, stable transformation, CRISPR/Cas9-mediated knockout, an anthocyanin-activating R2R3-myeloblastosis (MYB) gene, Purple Central Flower (DcPCF), was identified causal gene that triggers only to possess phenotype. The expression of DcPCF detected tiny flowers. We propose transition from nonpurple center umbel occurred after 3 separate adverse events: insertion transposons promoter region, premature termination coding sequence (caused by a C–T substitution open reading frame), emergence unknown anthocyanin suppressors. These events could either consecutively independently. intriguing its underlying mechanism may provide evidence it remnant ancient conditions species, reflecting original appearance Umbelliferae (also called Apiaceae) when single present.

Язык: Английский

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ABIOTIC STRESS GENE 1 mediates aroma volatiles accumulation by activating MdLOX1a in apple

Horticulture Research, Год журнала: 2024, Номер 11(10)

Опубликована: Авг. 8, 2024

Fruit aroma is an important organoleptic quality, which influences consumer preference and market competitiveness. Aroma compound synthesis pathways in plants have been widely identified, among the lipoxygenase pathway crucial for fatty acid catabolism to form esters apple. However, regulatory mechanism of this remains elusive. In study, linear regression analysis transgene verification revealed that MdLOX1a involved ester biosynthesis. Yeast one-hybrid library screening indicated a protein, MdASG1 (ABIOTIC STRESS GENE 1), was positive regulator

Язык: Английский

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Regulation of Proanthocyanidin Accumulation by Vvmyb1 in Response to Vine Water Constraint in 'Marselan' Grape Berries

Опубликована: Янв. 1, 2025

Язык: Английский

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Systematic identification of R2R3-MYB S6 subfamily genes in Brassicaceae and its role in anthocyanin biosynthesis in Brassica crops

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 6, 2025

The Brassicaceae family includes Arabidopsis thaliana, various vegetables and oil crops. R2R3-MYB genes of the S6 subfamily are crucial for regulating anthocyanin biosynthesis, however, their systematic identification in plants is still incomplete. Here, we systematically identified homologous transcription factors from across 31 species. A total 92 were identified, with species representation ranging 0 to 10 per Phylogenetic analysis classified these into six distinct groups. Notably, approximately 70% found within G6 group, indicating a high degree evolutionary conservation. Furthermore, phylogenetic was conducted on 35 obtained U's triangle Brassica plants. findings provided evidence significant conservation among orthologous demonstrated strong collinearity subgenomic chromosomes, notable tandem duplications observed chromosomes A7 C6. Subsequently, predicted cis-acting elements genes, analyzed structures, conserved motifs, characteristic domains, confirming similarities between genes. Additionally, employed white purple flower rapeseed specimens conduct qRT-PCR validation key transcriptional regulators associated synthesis pathway. results revealed differential expression BnaPAP2.A7.b flowers, alongside BnaPAP2.C6.d. Ultimately, based previous research this study, propose regulatory framework govern accumulation tissues or organs B. napus. Our offer novel perspective functional diversification while also shedding light network governing biosynthesis

Язык: Английский

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