Agricultural Sciences,
Год журнала:
2024,
Номер
15(02), С. 256 - 273
Опубликована: Янв. 1, 2024
Studying
on
the
genetic
diversity
and
relationship
of
flowering
cherry
cultivars
is
extremely
important
for
germplasm
conservation,
cultivar
identification
breeding.
Flowering
widely
cultivated
as
an
woody
ornamental
plant
in
worldwide,
especially
Japan,
China.
However,
owning
to
morphological
similarity,
many
are
distinguished
hardly
non-flowering
season.
Here,
we
evaluated
40
cultivars,
which
mainly
We
selected
13
polymorphicprimers
amplify
allele
fragments
with
fluorescent-labeled
capillary
electrophoresis
technology.
The
population
structure
analysis
results
show
that
these
could
be
divided
into
4
subpopulations.
At
level,
Na
Ne
were
6.062,
4.326,
respectively.
Ho
He
0.458
0.670,
Shannon’s
information
index
(I)
was
1.417.
Pop3,
originated
from
P.
serrulata,
had
highest
Ho,
He,
I
among
AMOVA
showed
only
4%
variation
came
populations,
39%
individuals
57%
(p
<
0.05)
intra-individuals.
5
polymorphic
SSR
primers
construct
molecular
ID
code
system
cultivars.
This
will
help
insight
background,
promote
identify
similar
Plants,
Год журнала:
2024,
Номер
13(19), С. 2697 - 2697
Опубликована: Сен. 26, 2024
SERK
is
a
marker
gene
for
early
somatic
embryogenesis.
We
screened
and
functionally
verified
SERK-interacting
protein
to
gain
insights
into
tree-peony
Using
PoSERK
as
bait,
we
identified
PorbcL
(i.e.,
the
large
subunit
of
Rubisco)
from
yeast
two-hybrid
(Y2H)
library
cDNA
developing
embryos.
The
interaction
between
was
by
Y2H
bimolecular
fluorescence
complementation
analyses.
encodes
586-amino-acid
acidic
non-secreted
hydrophobic
non-transmembrane
that
mainly
localized
in
chloroplast
plasma
membrane.
highly
expressed
roots
flowers
up-regulated
during
zygotic
embryo
development.
overexpression
caused
up-regulation
(encoding
embryogenesis
receptor-like
kinase),
PoAGL15
agamous-like
15),
PoGPT1
glucose-6-phosphate
translocator),
while
it
down-regulation
PoLEC1
leafy
cotyledon
1)
callus.
led
increased
indole-3-acetic
acid
(IAA)
content
but
decreasing
contents
abscisic
(ABA)
6-benzyladenosine
(BAPR).
changes
expression,
high
IAA
levels,
ratio
ABA,
BAPR,
1-Aminocyclopropanecarboxylic
(ACC),
5-Deoxystrigol
(5DS),
brassinolide
(BL)
promoted
These
results
provide
foundation
establishing
embryogenic
callus
system.
Agricultural Sciences,
Год журнала:
2024,
Номер
15(02), С. 256 - 273
Опубликована: Янв. 1, 2024
Studying
on
the
genetic
diversity
and
relationship
of
flowering
cherry
cultivars
is
extremely
important
for
germplasm
conservation,
cultivar
identification
breeding.
Flowering
widely
cultivated
as
an
woody
ornamental
plant
in
worldwide,
especially
Japan,
China.
However,
owning
to
morphological
similarity,
many
are
distinguished
hardly
non-flowering
season.
Here,
we
evaluated
40
cultivars,
which
mainly
We
selected
13
polymorphicprimers
amplify
allele
fragments
with
fluorescent-labeled
capillary
electrophoresis
technology.
The
population
structure
analysis
results
show
that
these
could
be
divided
into
4
subpopulations.
At
level,
Na
Ne
were
6.062,
4.326,
respectively.
Ho
He
0.458
0.670,
Shannon’s
information
index
(I)
was
1.417.
Pop3,
originated
from
P.
serrulata,
had
highest
Ho,
He,
I
among
AMOVA
showed
only
4%
variation
came
populations,
39%
individuals
57%
(p
<
0.05)
intra-individuals.
5
polymorphic
SSR
primers
construct
molecular
ID
code
system
cultivars.
This
will
help
insight
background,
promote
identify
similar