Analysis on Genetic Diversity of 40 Flowering Cherry Cultivars and Construction of Molecular ID Based on SSR Markers DOI Open Access
Chaoren Nie,

Xiaoguo Xu,

Xiaoqin Zhang

и другие.

Agricultural Sciences, Год журнала: 2024, Номер 15(02), С. 256 - 273

Опубликована: Янв. 1, 2024

Studying on the genetic diversity and relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification breeding. Flowering widely cultivated as an woody ornamental plant in worldwide, especially Japan, China. However, owning to morphological similarity, many are distinguished hardly non-flowering season. Here, we evaluated 40 cultivars, which mainly We selected 13 polymorphicprimers amplify allele fragments with fluorescent-labeled capillary electrophoresis technology. The population structure analysis results show that these could be divided into 4 subpopulations. At level, Na Ne were 6.062, 4.326, respectively. Ho He 0.458 0.670, Shannon’s information index (I) was 1.417. Pop3, originated from P. serrulata, had highest Ho, He, I among AMOVA showed only 4% variation came populations, 39% individuals 57% (p < 0.05) intra-individuals. 5 polymorphic SSR primers construct molecular ID code system cultivars. This will help insight background, promote identify similar

Язык: Английский

Functional Analysis of the PoSERK-Interacting Protein PorbcL in the Embryogenic Callus Formation of Tree Peony (Paeonia ostii T. Hong et J. X. Zhang) DOI Creative Commons
Yinglong Song, Jian‐Ge Wang,

Jiale Zhu

и другие.

Plants, Год журнала: 2024, Номер 13(19), С. 2697 - 2697

Опубликована: Сен. 26, 2024

SERK is a marker gene for early somatic embryogenesis. We screened and functionally verified SERK-interacting protein to gain insights into tree-peony Using PoSERK as bait, we identified PorbcL (i.e., the large subunit of Rubisco) from yeast two-hybrid (Y2H) library cDNA developing embryos. The interaction between was by Y2H bimolecular fluorescence complementation analyses. encodes 586-amino-acid acidic non-secreted hydrophobic non-transmembrane that mainly localized in chloroplast plasma membrane. highly expressed roots flowers up-regulated during zygotic embryo development. overexpression caused up-regulation (encoding embryogenesis receptor-like kinase), PoAGL15 agamous-like 15), PoGPT1 glucose-6-phosphate translocator), while it down-regulation PoLEC1 leafy cotyledon 1) callus. led increased indole-3-acetic acid (IAA) content but decreasing contents abscisic (ABA) 6-benzyladenosine (BAPR). changes expression, high IAA levels, ratio ABA, BAPR, 1-Aminocyclopropanecarboxylic (ACC), 5-Deoxystrigol (5DS), brassinolide (BL) promoted These results provide foundation establishing embryogenic callus system.

Язык: Английский

Процитировано

1

Analysis on Genetic Diversity of 40 Flowering Cherry Cultivars and Construction of Molecular ID Based on SSR Markers DOI Open Access
Chaoren Nie,

Xiaoguo Xu,

Xiaoqin Zhang

и другие.

Agricultural Sciences, Год журнала: 2024, Номер 15(02), С. 256 - 273

Опубликована: Янв. 1, 2024

Studying on the genetic diversity and relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification breeding. Flowering widely cultivated as an woody ornamental plant in worldwide, especially Japan, China. However, owning to morphological similarity, many are distinguished hardly non-flowering season. Here, we evaluated 40 cultivars, which mainly We selected 13 polymorphicprimers amplify allele fragments with fluorescent-labeled capillary electrophoresis technology. The population structure analysis results show that these could be divided into 4 subpopulations. At level, Na Ne were 6.062, 4.326, respectively. Ho He 0.458 0.670, Shannon’s information index (I) was 1.417. Pop3, originated from P. serrulata, had highest Ho, He, I among AMOVA showed only 4% variation came populations, 39% individuals 57% (p < 0.05) intra-individuals. 5 polymorphic SSR primers construct molecular ID code system cultivars. This will help insight background, promote identify similar

Язык: Английский

Процитировано

0