Partial unidirectional translocation from 5AL to 7BS leads to dense spike in an EMS-induced wheat mutant DOI Creative Commons
Xiaoyu Zhang, Yongfa Wang, Yongming Chen

и другие.

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Ноя. 12, 2024

As the inflorescence of wheat, spike architecture largely determines grain productivity. Dissecting genetic basis for morphology wheat can contribute to designation ideal improve production. The present study characterizes a dense spike1 (ds1) mutant, derived from Nongda3753, induced by EMS treatment, which exhibits and reduced plant height. Through bulked segregant analysis sequencing (BSA-Seq) two segregating populations, ds1 was mapped short arm chromosome 7B. Further genotypic phenotypic analyses residual heterozygous lines F3 F6 Yong3002×ds1 revealed that there 0-135 Mb deletion in 7B associated with phenotype. reads count bulks BSA-Seq, along cytological ds1, ND3753, NIL-ds1 NIL-Y3002, confirmed partial unidirectional translocation 5AL (543–713 Mb) 7BS (0-135 exists ds1. This led an increase both copy number expression Q gene, is one reasons phenotype observed Partial identified EMS-induced mutant research illustrates effect structure variation on morphology, provides new materials several variations future breeding.

Язык: Английский

Development and characterization of small translocations between the Pm13 introgression from Aegilops longissima and wheat homoeologous A, B and D chromosomes DOI Creative Commons
Shunzong Ning,

Xiyou Wang,

Chunfang Ma

и другие.

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 11, 2025

Wild relatives have substantial impacts on the resistance of wheat to biotic and abiotic stresses. The genetic diversity these wild varieties can be employed widen gene pool by introducing allele introgression genome structure variations. powdery mildew Pm13 was derived from relative species Aegilops longissima (S1S1, 2n = 2x 14) transferred into chromosome 3BS over 30 years. Until recently, it has not been widely used in breeding, possibly because linkage drag associated with or poor alien segment compensation 3BS. In this study, translocations between homoeologous A, B D chromosomes were developed using a ph1b mutant. 3Sl (3B) substitution line hybridized CS nullisomic-tetrasomic N3AT3B, then F1 CSph1b Individuals homozygous for mutant carrying identified segregating populations via molecular markers cytological analysis. Whole-arm recombinant T3SlS.3AL T3SlS.3BL small T3SlS-3AS.3AL, T3SlS-3BS.3BL T3DS-3SlS-3DS.3DL subsequently Furthermore, 4 disomic recombinants identified. All nearly immune mildew. Our results provide novel improving insights interactions different genomes segments.

Язык: Английский

Процитировано

0
Analysis of the molecular karyotype of Kengyilia grandiglumis on the Qinghai-Tibet Plateau via sequential FISH and GISH DOI
Ruijuan Liu,

Liling Jiang,

Yijun Zhong

и другие.

Genetic Resources and Crop Evolution, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Язык: Английский

Процитировано

0
The near-complete genome assembly of hexaploid wild oat reveals its genome evolution and divergence with cultivated oats DOI
Qiang He, Wei Li, Yuqing Miao

и другие.

Nature Plants, Год журнала: 2024, Номер unknown

Опубликована: Дек. 3, 2024

Язык: Английский

Процитировано

2
Genome-wide expansion and reorganization during grass evolution: from 30 Mb chromosomes in rice and Brachypodium to 550 Mb in Avena DOI Creative Commons
Qing Liu,

Lyuhan Ye,

Mingzhi Li

и другие.

BMC Plant Biology, Год журнала: 2023, Номер 23(1)

Опубликована: Дек. 8, 2023

The BOP (Bambusoideae, Oryzoideae, and Pooideae) clade of the Poaceae has a common ancestor, with similarities to genomes rice, Oryza sativa (2n = 24; genome size 389 Mb) Brachypodium, Brachypodium distachyon 10; 271 Mb). We exploit chromosome-scale assemblies show nature genomic expansion, structural variation, chromosomal rearrangements from rice diploids in tribe Aveneae (e.g., Avena longiglumis, 2n 2x 14; 3,961 Mb assembled 3,850 chromosomes).Most chromosome arms relatively uniform expansion over 10-fold 15-fold genome-size increase. Apart non-coding sequence diversification accumulation around centromeres, blocks genes are not interspersed repeats, even subterminal regions. As Triticeae, conserved synteny seen between analyzed species fusion, fission, nesting (insertion) events showing deep evolutionary conservation structure during expansion. Unexpectedly, terminal gene-rich segments (representing about 50 translocations chromosomes speciation, homogenization genome-specific repetitive elements within Aveneae. Newly-formed intergenomic similar extent found hexaploid A. sativa.The study provides insight into mechanisms speciation clade, which is valuable for measurement biodiversity, development clade-wide pangenome, exploitation diversity through breeding programs Poaceae.

Язык: Английский

Процитировано

4
Structural chromosome variations from Jinmai 47 and Jinmai 84 affected agronomic traits and drought tolerance of wheat DOI Creative Commons

Shuwei Zhang,

Jiajia Zhao,

Haiyan Zhang

и другие.

Journal of Integrative Agriculture, Год журнала: 2024, Номер unknown

Опубликована: Июль 1, 2024

Structural variation is an important source of genetic in wheat and have been the evolution wheat's genome. Few studies examined relationship between structural variations agronomy drought tolerance. The present study identified chromosome (SCVs) a doubled haploid (DH) population backcross introgression lines (BC5F3) derived from Jinmai 47 84 using fluorescence situ hybridization. There are one simple translocation, 10 present/absent (PAVs), copy number (CNV) 84, which distributed chromosomes. Eight SCVs were associated with 15 agronomic traits. A PAV recombination occurred on 2A, was grain per spike (GNS). 1BL/1RS translocation PAV.2D significant reductions plant height, deriving effects LI2-LI4 UI, LI2-LI4, respectively. also contributed to increase 3.13% for GNS, significantly increased spikelet number, length (GL), thickness (GT). effect PAV.4A.1 GL, PAV.6A (SL) thousand-grain weight (TGW), PAV.6B SL, GT TGW verified. PAVs chromosomes 6A, 1D, 2D, CNV 4B tolerance coefficients. Additive interaction among observed. Many previously cloned key genes yield-related QTL found polymorphic regions PAV.2B, PAV.2D, CNV.4B. Altogether, this confirmed tolerance, identification these will facilitate improvement through marker-assisted selection.

Язык: Английский

Процитировано

0
Karyotypic and phenotypic condensation in allotetraploid wheats accompanied with reproductive strategy transformation: from natural evolution to domestication DOI
Wei Fan,

Meiqi Sun,

Yongbao Zheng

и другие.

Planta, Год журнала: 2024, Номер 260(4)

Опубликована: Авг. 30, 2024

Язык: Английский

Процитировано

0
Partial unidirectional translocation from 5AL to 7BS leads to dense spike in an EMS-induced wheat mutant DOI
Xiaoyu Zhang, Yongfa Wang, Yongming Chen

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 17, 2024

Abstract Background As the inflorescence of wheat, spike architecture largely determines grain productivity. Dissecting genetic basis for morphology wheat can contribute towards designation ideal to improve production. Results In this study, we characterized an EMS-induced mutant dense spike1 (ds1) from Nongda3753 (ND3753) with a dense and reduced plant height. Using bulked segregant analysis coupled whole-genome sequencing (BSA-Seq) two segregating populations, ds1 was mapped short arm chromosome 7B. Further genotypic phenotypic analyses residual heterozygous lines F3 F6 Yong3002×ds1 revealed that there 0-135Mb deletion in 7B associated phenotype. The reads count bulks BSA-Seq along cytological ds1, ND3753, NIL-ds1 NIL-Y3002 confirmed partial unidirectional translocation 5AL (541-713Mb) 7BS (0-135Mb) ds1. This resulted increase copy number expression Q gene, thereby leading phenotype observed Conclusion We identified which exhibiting research deepens our understanding dosage-dependent effect gene on morphology, provides new materials several structural variations breeding.

Язык: Английский

Процитировано

0
Partial unidirectional translocation from 5AL to 7BS leads to dense spike in an EMS-induced wheat mutant DOI Creative Commons
Xiaoyu Zhang, Yongfa Wang, Yongming Chen

и другие.

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Ноя. 12, 2024

As the inflorescence of wheat, spike architecture largely determines grain productivity. Dissecting genetic basis for morphology wheat can contribute to designation ideal improve production. The present study characterizes a dense spike1 (ds1) mutant, derived from Nongda3753, induced by EMS treatment, which exhibits and reduced plant height. Through bulked segregant analysis sequencing (BSA-Seq) two segregating populations, ds1 was mapped short arm chromosome 7B. Further genotypic phenotypic analyses residual heterozygous lines F3 F6 Yong3002×ds1 revealed that there 0-135 Mb deletion in 7B associated with phenotype. reads count bulks BSA-Seq, along cytological ds1, ND3753, NIL-ds1 NIL-Y3002, confirmed partial unidirectional translocation 5AL (543–713 Mb) 7BS (0-135 exists ds1. This led an increase both copy number expression Q gene, is one reasons phenotype observed Partial identified EMS-induced mutant research illustrates effect structure variation on morphology, provides new materials several variations future breeding.

Язык: Английский

Процитировано

0