Phytochrome-interacting factors PIF4 and PIF5 directly regulate autophagy during leaf senescence in Arabidopsis DOI
Juhyeon Lee,

Myeong Hoon Kang,

Da‐Min Choi

и другие.

Journal of Experimental Botany, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 16, 2024

Abstract During leaf senescence, autophagy plays a critical role by removing damaged cellular components and participating in nutrient remobilization to sink organs. However, how AUTOPHAGY (ATG) genes are regulated during natural senescence remains largely unknown. In this study, we attempted identify upstream transcriptional regulator(s) of ATG their molecular basis Arabidopsis through the combined analyses promoter binding, flux, genetic interactions. We found that PHYTOCHROME-INTERACTING FACTOR4 (PIF4) PIF5 directly bind promoters ATG5, ATG12a, ATG12b, ATG8a, ATG8e, ATG8f, ATG8g, inducing transcription. These target down-regulated pif4, pif5, pif4pif5 mutants, resulting decreased autophagic activity slower degradation chloroplast proteins chlorophyll. Conversely, overexpression ATG8 accelerated protein with early senescence. Moreover, our data suggested partial suppression phenotype ATG8a overexpression. PIF4/PIF5 also influence induced starvation, another hallmark pathway. Furthermore, observed PIF4/PIF5–ATG regulatory module may contribute seed maturation. Our study not only unveils regulators but underscores potential as functional remobilization.

Язык: Английский

How abiotic stresses trigger sugar signaling to modulate leaf senescence? DOI

Muhmmad Asad Ullah Asad,

Yan Zhang,

Lujian Zhou

и другие.

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 210, С. 108650 - 108650

Опубликована: Апрель 21, 2024

Язык: Английский

Процитировано

14
Integrated analysis of postharvest storage characteristics of seven apple cultivars and transcriptome data identifies MdBBX25 as a negative regulator of fruit softening during storage in apples DOI Open Access
Kaisheng Zhang, Dongyang Zhang,

Xuekun Wang

и другие.

Postharvest Biology and Technology, Год журнала: 2023, Номер 207, С. 112646 - 112646

Опубликована: Ноя. 1, 2023

Язык: Английский

Процитировано

17
Light controls mesophyll-specific post-transcriptional splicing of photoregulatory genes by AtPRMT5 DOI Creative Commons
Yan Yan, H. Luo,

Yuwei Qin

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(6)

Опубликована: Янв. 29, 2024

Light plays a central role in plant growth and development, providing an energy source governing various aspects of morphology. Previous study showed that many polyadenylated full-length RNA molecules within the nucleus contain unspliced introns (post-transcriptionally spliced introns, PTS introns), which may play rapidly responding to changes environmental signals. However, mechanism underlying post-transcriptional regulation during initial light exposure young, etiolated seedlings remains elusive. In this study, we used FLEP-seq2, Nanopore-based sequencing technique, analyze nuclear RNAs Arabidopsis ( thaliana ) under different conditions found numerous light-responsive introns. We also single-nucleus (snRNA-seq) profile transcripts single investigate distribution across distinct cell types. established light-induced are predominant mesophyll cells seedling de-etiolation following light. further demonstrated involvement splicing-related factor A. PROTEIN ARGININE METHYLTRANSFERASE 5 (AtPRMT5), working concert with E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), critical repressor signaling pathways. these two proteins orchestrate events facilitate chloroplast photosynthesis, morphogenesis response ever-changing conditions. These findings provide crucial insights into intricate mechanisms acclimation at cell-type level.

Язык: Английский

Процитировано

5
Identification and functional characterization of the MYB transcription factor GmMYBLJ in soybean leaf senescence DOI Creative Commons

Guohua Bao,

Xu Xiao, Jingyu Yang

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Янв. 24, 2025

Leaf senescence is an important agronomic trait that significantly influences the quality and yield of soybeans. v-Myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors are considered crucial regulators governing leaf senescence, which can be utilized to improve traits in crops. However, our knowledge regarding functional roles soybean MYBs extremely limited. In this study, GmMYBLJ , a CCA1-like MYB was identified functionally characterized with respect senescence. The protein localized nucleus, high accumulation its transcripts observed nodules embryos. Notably, highly expressed senescent leaves transcriptionally induced by dark or NaCl treatment, as confirmed histochemical GUS staining analysis. Ectopic overexpression Arabidopsis not only led earlier reduced chlorophyll content, increased MDA but also promoted expression several WRKY family senescence-associated genes, such SAG12 ORE1 . Further investigation showed accelerated under darkness response Pst DC3000 infection. Moreover, transgenic plants overexpressing grew faster exhibited salt stress. DAB analysis ROS hairy roots leaves. Collectively, results provided useful information into both age-dependent stress-induced

Язык: Английский

Процитировано

0
Genome-Wide Identification of B-Box Gene Family and Candidate Light-Related Member Analysis of Tung Tree (Vernicia fordii) DOI Open Access
Kai Shi, Guang Zhao, Ze Li

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 1977 - 1977

Опубликована: Фев. 6, 2024

Light is one of the most important environmental factors for plant growth. In production process tung oil tree cultivation, due to inappropriate growth shading conditions, lower branches are often dry and dead, which seriously affects yield trees. However, little known about key light-induced photomorphogenesis. this study, a total 22 VfBBX family members were identified provide reference candidate genes in seedlings. All have different numbers highly conserved B-box domains or CCT domains. Phylogenetic evolution clustered into four categories, highest density was on chromosome 6. Interspecific collinearity analysis suggested that there six pairs duplicate members, but expression levels all development stages significantly divergent. After degrees treatment physiological data determination seedlings, differential level chlorophyll synthesis correlation revealed VfBBX9 typical nuclear localization transcription factor differentially expressed light response. This study systematically gene provided studying its molecular function, enhanced theoretical basis breeding, excellent varieties.

Язык: Английский

Процитировано

3
HASTY-mediated miRNA dynamics modulate nitrogen starvation-induced leaf senescence in Arabidopsis DOI Creative Commons
Yasuhito Sakuraba, Mailun Yang, Shuichi Yanagisawa

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 10, 2024

Язык: Английский

Процитировано

3
MYB44 plays key roles in regulating plant responses to abiotic and biotic stress, metabolism, and development DOI
Fenghua Wang, Feng Yang,

Dengfeng Zhu

и другие.

Journal of Plant Biochemistry and Biotechnology, Год журнала: 2023, Номер unknown

Опубликована: Дек. 2, 2023

Язык: Английский

Процитировано

7
Arabidopsis BBX14 is involved in high light acclimation and seedling development DOI Creative Commons
Vasil Atanasov, Julia Schumacher, José M. Muiño

и другие.

The Plant Journal, Год журнала: 2023, Номер 118(1), С. 141 - 158

Опубликована: Дек. 21, 2023

The development of photosynthetically competent seedlings requires both light and retrograde biogenic signaling pathways. transcription factor GLK1 functions at the interface between these pathways receives input from signal integrator GUN1. BBX14 was previously identified, together with GLK1, in a core module that mediates response to high (HL) levels signals, which studied by using inhibitors chloroplast development. Our chromatin immunoprecipitation-Seq experiments revealed is direct target RNA-Seq analysis suggests may function as regulator circadian clock. In addition, plays role chlorophyll biosynthesis during early onset light. Knockout results long hypocotyl phenotype dependent on signal. Furthermore, expression BBX15 Investigation GUN-type (gun) showed overexpression or caused de-repression CA1 mRNA levels, when were grown norflurazon. Notably, transcripts LHCB1.2 marker are not de-repressed. required acclimate plants HL stress. We propose an signals nuclear downstream GUN1/GLK1 module. However, we do classify overexpressors gun mutants based critical evaluation our those reported literature. Finally, discuss classification system necessary for declaration new mutants.

Язык: Английский

Процитировано

5
ChBBX6 and ChBBX18 are positive regulators of anthocyanins biosynthesis and carotenoids degradation in Cerasus humilis DOI

Yang Hu,

Zhong‐Hua Gong,

Yiming Yan

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 282, С. 137195 - 137195

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

1
The BBX family and their response to abiotic stress in ginger (Zingiber officinale Roscoe) DOI
Jiayu Shi,

Yun-Rui Tang,

Hong‐Lei Li

и другие.

Опубликована: Июнь 27, 2024

Abstract Background Ginger (Zingiber officinale Roscoe), a medicinal and edible plant in Chinese culture, is often impacted by adverse environmental conditions. BBX genes are fundamental regulators of multiple pathways plants, although systematic cataloging ginger has not yet been completed. Result .The study examined 31 members the gene family (ZoBBXs). Moreover, comprehensive analysis conducted on sequence features, conserved protein motifs, chromosome location, phylogenetic relationships, duplication events ZoBBXs. In terms structures, domains, ZoBBXs can be categorized into five categories. investigated expression patterns across different developmental stages under various abiotic stresses, such as ABA, cold, drought, heat, salt, utilizing RNA-seq data qRT-PCR analysis. The results indicated that ZoBBX#04, ZoBBX#11, ZoBBX#24, ZoBBX#27 may key flowering ginger, while ZoBBX#05, ZoBBX#17, ZoBBX#24 were identified significant contributors to stress response mechanisms. Conclusion A detailed ginger's presented this study, laying groundwork for further exploration potential roles ZoBBX growth, development, tolerance stresses.

Язык: Английский

Процитировано

0