The Isodon serra genome sheds light on tanshinone biosynthesis and reveals the recursive karyotype evolutionary histories within Lamiales DOI
Liqiang Hou,

Zhimin Niu,

Zeyu Zheng

и другие.

The Plant Journal, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 30, 2024

SUMMARY Lamiales is one of the largest orders angiosperms with a complex evolutionary history and plays significant role in human life. However, polyploidization chromosome evolution histories within this group remain mystery. Among Lamiales, Isodon serra (Maxim.) Kudô shines for its abundance diterpenes, notably tanshinones, long used East Asia to combat toxicity inflammation. Yet, genes driving biosynthesis factors governing regulation linger obscurity. Here, we present telomere‐to‐telomere genome assembly I. and, through gene‐to‐metabolite network analyses, pinpoint pivotal tanshinone their co‐expressed transcription factors. Particularly, luciferase (LUC) assays, speculate that IsMYB‐13 IsbHLH‐8 may upregulate IsCYP76AH101 , which key step precursor. Oleaceae, Gesneriaceae Plantaginaceae successively sister clade seven families, all sharing recent whole‐genome duplication (designated as α event). By reconstructing ancestral karyotypes (ALK) post‐α event (ALKα), trace chromosomal trajectories across species. Notably, fusion detected from ALK ALKα, three shared events are sequentially ALKα fully supports phylogeny constructed using single‐copy genes. This comprehensive study illuminates dynamics further enhancing our understanding biosynthetic mechanisms underlying medicinal properties serra.

Язык: Английский

Genome mining and characterization of bifunctional Clerodane Diterpene synthase from a fungus Myrothecium sp DOI
Shuxuan Li, Ke Ma, Yujie Zhao

и другие.

Bioorganic Chemistry, Год журнала: 2025, Номер 161, С. 108548 - 108548

Опубликована: Апрель 29, 2025

Язык: Английский

Процитировано

0
Sequence-Structure Analysis Unlocking the Potential Functional Application of the Local 3D Motifs of Plant-Derived Diterpene Synthases DOI Creative Commons

Yalan Zhao,

Yupeng Liang,

Gan Luo

и другие.

Biomolecules, Год журнала: 2024, Номер 14(1), С. 120 - 120

Опубликована: Янв. 17, 2024

Plant-derived diterpene synthases (PdiTPSs) play a critical role in the formation of structurally and functionally diverse diterpenoids. However, specificity or functional-related features PdiTPSs are not well understood. For more profound insight, we collected, constructed, curated 199 characterized their corresponding 3D structures. The complex correlations among sequences, domains, structures, products were comprehensively analyzed. Ultimately, our focus narrowed to geometric arrangement local We found that structural alignment can rapidly localize product-specific residues have been validated by mutagenesis experiments. Based on motifs derived from around substrate, successfully searched (diTPSs) predicted terpene newly PdiTPSs, suggesting identified serve as distinctive signatures diTPSs (I II class). Local analysis revealed with conserved amino acid show unique class I II, whereas those fewer typically exhibit product diversity specificity. These results provide an attractive method for discovering novel equivalent enzymes probing cases where enzyme characterization is limited.

Язык: Английский

Процитировано

1
CYP76BK1 orthologs catalyze furan and lactone ring formation in clerodane diterpenoids across the mint family DOI Creative Commons
Nicholas Schlecht, Emily R. Lanier, Trine B. Andersen

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 29, 2024

SUMMARY The Lamiaceae (mint family) is the largest known source of furanoclerodanes, a subset clerodane diterpenoids with broad bioactivities including insect antifeedant properties. Ajugoideae subfamily, in particular, accumulates significant numbers structurally related furanoclerodanes. biosynthetic capacity for formation these retained across most subfamilies, early-diverging Callicarpoideae which forms sister clade to rest Lamiaceae. VacCYP76BK1 , cytochrome P450 monooxygenase from Vitex agnus-castus was previously found catalyze proposed precursor furan and lactone-containing labdane diterpenoids. Through transcriptome-guided pathway exploration, we identified orthologs Ajuga reptans Callicarpa americana. Functional characterization demonstrated that both could oxidative cyclization backbones yield ring. Subsequent investigation revealed total ten CYP76BK1 six subfamilies. analysis available chromosome-scale genomes, four members as syntelogs within conserved syntenic block divergent This suggests an evolutionary lineage predates speciation affirmed conservation function, all catalyzed ring formation. Additionally, some yielded two novel lactone moieties. presence subfamilies closely overlaps distribution reported Together, activities here support their central role furanoclerodane biosynthesis family. Our findings lay groundwork biotechnological applications harness economic potential this promising class compounds. Significance Statement discovery functional diverse chemistry biosynthesis, providing insights into metabolic landscape dynamic evolution plant family over approximately 50 million years. These pave way targeted engineering efforts sustainable production compounds, offering prospects agricultural pharmaceutical applications.

Язык: Английский

Процитировано

0
Genomic analysis of secondary metabolite biosynthesis gene clusters and structural characterization of terpene synthase and cytochrome P450 enzymes in Zingiber officinale Roscoe DOI Creative Commons
Ummahan Öz

Journal of New Results in Science, Год журнала: 2024, Номер 13(2), С. 134 - 152

Опубликована: Авг. 31, 2024

This study uses bioinformatics approaches to elucidate the genetic basis of secondary metabolite biosynthesis in Zingiber officinale (Z. officinale). To this end, it identifies 44 biosynthetic gene clusters and maps onto individual chromosomes, with chromosomes 1A 8A exhibiting higher concentrations. Here, protein homology modeling provided insights into structural characteristics terpene synthases Cytochrome P450 enzymes, shedding light on their potential roles stress response production. Moreover, identification such as (-)-kolavenyl diphosphate synthase TPS28 cytochrome 93A3-like, opens up new possibilities for investigating intricate pathways involved diversity mechanisms within Z. officinale. highlights importance understanding molecular underlying plant-derived bioactive compounds pharmaceutical applications.

Язык: Английский

Процитировано

0
CYP76BK1 orthologs catalyze furan and lactone ring formation in clerodane diterpenoids across the mint family DOI Creative Commons
Nicholas Schlecht, Emily R. Lanier, Trine B. Andersen

и другие.

The Plant Journal, Год журнала: 2024, Номер 120(3), С. 984 - 997

Опубликована: Сен. 14, 2024

SUMMARY The Lamiaceae (mint family) is the largest known source of furanoclerodanes, a subset clerodane diterpenoids with broad bioactivities including insect antifeedant properties. Ajugoideae subfamily, in particular, accumulates significant numbers structurally related furanoclerodanes. biosynthetic capacity for formation these retained across most subfamilies, early‐diverging Callicarpoideae which forms sister clade to rest Lamiaceae. VacCYP76BK1 , cytochrome P450 monooxygenase from Vitex agnus‐castus was previously found catalyze proposed precursor furan and lactone‐containing labdane diterpenoids. Through transcriptome‐guided pathway exploration, we identified orthologs Ajuga reptans Callicarpa americana. Functional characterization demonstrated that both could oxidative cyclization backbones yield ring. Subsequent investigation revealed total 10 CYP76BK1 six subfamilies. analysis available chromosome‐scale genomes, four members as syntelogs within conserved syntenic block divergent This suggests an evolutionary lineage predates speciation affirmed conservation function, all catalyzed ring formation. Additionally, some yielded two novel lactone moieties. presence subfamilies closely overlaps distribution reported Together, activities here support their central role furanoclerodane biosynthesis family. Our findings lay groundwork biotechnological applications harness economic potential this promising class compounds.

Язык: Английский

Процитировано

0
Identification and functional characterization of the diterpene synthase family in Pogostemon cablin (Blanco) Benth DOI
Yiqiong Chen,

Yu-Min Lin,

Yingying Qiu

и другие.

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 216, С. 109190 - 109190

Опубликована: Окт. 10, 2024

Язык: Английский

Процитировано

0
The Isodon serra genome sheds light on tanshinone biosynthesis and reveals the recursive karyotype evolutionary histories within Lamiales DOI
Liqiang Hou,

Zhimin Niu,

Zeyu Zheng

и другие.

The Plant Journal, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 30, 2024

SUMMARY Lamiales is one of the largest orders angiosperms with a complex evolutionary history and plays significant role in human life. However, polyploidization chromosome evolution histories within this group remain mystery. Among Lamiales, Isodon serra (Maxim.) Kudô shines for its abundance diterpenes, notably tanshinones, long used East Asia to combat toxicity inflammation. Yet, genes driving biosynthesis factors governing regulation linger obscurity. Here, we present telomere‐to‐telomere genome assembly I. and, through gene‐to‐metabolite network analyses, pinpoint pivotal tanshinone their co‐expressed transcription factors. Particularly, luciferase (LUC) assays, speculate that IsMYB‐13 IsbHLH‐8 may upregulate IsCYP76AH101 , which key step precursor. Oleaceae, Gesneriaceae Plantaginaceae successively sister clade seven families, all sharing recent whole‐genome duplication (designated as α event). By reconstructing ancestral karyotypes (ALK) post‐α event (ALKα), trace chromosomal trajectories across species. Notably, fusion detected from ALK ALKα, three shared events are sequentially ALKα fully supports phylogeny constructed using single‐copy genes. This comprehensive study illuminates dynamics further enhancing our understanding biosynthetic mechanisms underlying medicinal properties serra.

Язык: Английский

Процитировано

0