Microsystems & Nanoengineering,
Год журнала:
2024,
Номер
10(1)
Опубликована: Окт. 8, 2024
Understanding
complex
regulatory
networks
in
plant
systems
requires
elucidating
the
roles
of
various
gene
regulators
under
a
spatial
landscape.
MicroRNA
are
key
that
impart
high
information
value
through
their
tissue
specificity
and
stability
when
using
expression
patterns
for
evaluating
network
outcomes.
However,
current
techniques
utilize
multiplexing
quantitation
microRNA
limited
to
primarily
mammalian
systems.
Here,
we
present
method
spatially
resolve
quantify
multiple
endogenous
situ
ethanol
fixed,
paraffin
embedded
model
species.
This
utilizes
target-specific
capture
along
with
universal
ligating
labelling,
all
within
functionalized
hydrogel
posts
containing
DNA
probes
nanoliter
well
arrays.
We
demonstrate
platform's
capabilities
analyzing
three
Arabidopsis
thaliana
rosettes
which
provide
useful
answers
fundamental
growth
development
from
unique
patterns.
The
technique
is
also
validated
non-spatial
small
RNA
assays
versatility
array
platform.
Our
new
platform
expands
toolkit
omics
technologies
plants.
Journal of Experimental Botany,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 20, 2024
Abstract
The
extensive
use
of
nitrogen
fertilizers
has
detrimental
environmental
consequences,
and
it
is
essential
for
society
to
explore
sustainable
alternatives.
One
promising
avenue
engineering
root
nodule
symbiosis,
a
naturally
occurring
process
in
certain
plant
species
within
the
nitrogen-fixing
clade,
into
non-leguminous
crops.
Advancements
single-cell
transcriptomics
provide
unprecedented
opportunities
dissect
molecular
mechanisms
underlying
symbiosis
at
cellular
level.
This
review
summarizes
key
findings
from
studies
Medicago
truncatula,
Lotus
japonicus,
Glycine
max.
We
highlight
how
these
address
fundamental
questions
about
development
including
following
findings:
(i)
revealed
conserved
transcriptional
program
hair
cortical
cells
during
rhizobial
infection,
suggesting
common
infection
pathway
across
legume
species;
(ii)
characterization
determinate
indeterminate
nodules
using
technologies
supports
compartmentalization
fixation,
assimilation,
transport
distinct
cell
populations;
(iii)
data
have
enabled
identification
novel
genes
provided
new
approaches
prioritizing
candidate
functional
characterization;
(iv)
trajectory
inference
RNA
velocity
analyses
allowed
reconstruction
lineages
dynamic
states
symbiosis.
PLANT PHYSIOLOGY,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 17, 2024
Understanding
root
development
is
critical
for
enhancing
plant
growth
and
health,
advanced
technologies
are
essential
unraveling
the
complexities
of
these
processes.
In
this
review,
we
highlight
select
technological
innovations
in
study
development,
with
a
focus
on
transformative
impact
single-cell
gene
expression
analysis.
We
provide
high-level
overview
recent
advancements,
illustrating
how
RNA
sequencing
(scRNA-seq)
has
become
pivotal
tool
biology.
scRNA-seq
revolutionized
biology
by
enabling
detailed,
cell-specific
analysis
expression.
This
allowed
researchers
to
create
comprehensive
atlases,
predict
cell
map
regulatory
networks
(GRNs)
unprecedented
precision.
Complementary
technologies,
such
as
multimodal
profiling
bioinformatics,
further
enrich
our
understanding
cellular
dynamics
interactions.
Innovations
imaging
modeling,
combined
genetic
tools
like
CRISPR,
continue
deepen
knowledge
formation
function.
Moreover,
integration
biosensors
microfluidic
devices
ability
plant-microbe
interactions
phytohormone
signaling
at
high
resolution.
These
collectively
more
system
architecture
its
regulation
environmental
factors.
As
evolve,
they
promise
drive
breakthroughs
science,
substantial
implications
agriculture
sustainability.
Microsystems & Nanoengineering,
Год журнала:
2024,
Номер
10(1)
Опубликована: Окт. 8, 2024
Understanding
complex
regulatory
networks
in
plant
systems
requires
elucidating
the
roles
of
various
gene
regulators
under
a
spatial
landscape.
MicroRNA
are
key
that
impart
high
information
value
through
their
tissue
specificity
and
stability
when
using
expression
patterns
for
evaluating
network
outcomes.
However,
current
techniques
utilize
multiplexing
quantitation
microRNA
limited
to
primarily
mammalian
systems.
Here,
we
present
method
spatially
resolve
quantify
multiple
endogenous
situ
ethanol
fixed,
paraffin
embedded
model
species.
This
utilizes
target-specific
capture
along
with
universal
ligating
labelling,
all
within
functionalized
hydrogel
posts
containing
DNA
probes
nanoliter
well
arrays.
We
demonstrate
platform's
capabilities
analyzing
three
Arabidopsis
thaliana
rosettes
which
provide
useful
answers
fundamental
growth
development
from
unique
patterns.
The
technique
is
also
validated
non-spatial
small
RNA
assays
versatility
array
platform.
Our
new
platform
expands
toolkit
omics
technologies
plants.
