Biochemical and Transcriptomic Analyses Reveal Key Salinity and Alkalinity Stress Response and Tolerance Pathways in Salix linearistipularis Inoculated with Trichoderma

Agronomy, Год журнала: 2024, Номер 14(10), С. 2358 - 2358

Опубликована: Окт. 13, 2024

Soil salinization and alkalinization are pervasive environmental issues that severely restrict plant growth crop yield. Utilizing growth-promoting rhizobacteria (PGPR) is an effective strategy to enhance tolerance saline–alkaline stress, though the regulatory mechanisms remain unclear. This study employed biochemical RNA-Seq methods uncover critical effects of Trichoderma spp. on Salix linearistipularis under stress. The results showed that, during inoculation with sp. M4 M5 significantly increased proline soluble sugar contents in linearistipularis, enhanced activities SOD, POD, CAT, APX, reduced lipid peroxidation levels, exhibiting more pronounced than M5. analysis revealed 11,051 genes were upregulated after stress conditions, 3532 primarily involved carbon metabolism, amino acid biosynthesis, oxidative phosphorylation—processes alleviate Additionally, 7519 uniquely by mainly enriched secondary metabolite cyanamide phenylpropanoid biosynthesis. mitigates stress-induced damage seedlings reducing damage, enhancing organic activating biosynthesis pathways eliminate harmful ROS. enhances seedlings’ providing a basis for studying fungi–plant interactions such conditions.

Язык: Английский

Effect of organic waste material and rhizobacteria on growth and physiology of Brassica juncea (L.) Czern. under salinity stress

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 25, 2024

Salinity is one of the major environmental problems in arid and semi-arid region which affect plant physiological yield attributes. Organic waste materials such as banana peels eggshells contain essential nutrients that are necessary for growth development. The inoculation rhizobacteria allows to decrease hazardous effects salinity stress. present study was conducted evaluate potential organic material on physiology Brassica juncea (L.) Czern. under experiment consisted various treatments including (20g), peel (30ml Bacillus megaterium) NPK(0.033kgh^{− 1}). In current study, salt stress 0 mM 150 NaCl concentration applied. From outcome it shown caused changes morphological, physiological, biochemical parameters. Peel powder treatment megaterium strain play role regulators development Among treatments, significantly enhanced antioxidant activity (CAT, POD, SOD total soluble protein), phenolic, leaf proline, reduced oxidative markers (MDA, H₂O₂) promoted membrane stability However, carotenoids content increased NPK treatment.

Язык: Английский